2021
Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis
Park S, Matte-Martone C, Gonzalez DG, Lathrop EA, May DP, Pineda CM, Moore JL, Boucher JD, Marsh E, Schmitter-Sánchez A, Cockburn K, Markova O, Bellaïche Y, Greco V. Skin-resident immune cells actively coordinate their distribution with epidermal cells during homeostasis. Nature Cell Biology 2021, 23: 476-484. PMID: 33958758, PMCID: PMC8603572, DOI: 10.1038/s41556-021-00670-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEpidermal CellsEpidermisHomeostasisIntercellular JunctionsMice, TransgenicSkinT-LymphocytesConceptsDendritic epidermal T cellsLangerhans cellsCell typesEpithelial cellsDifferent cell typesMultiple cell typesLive adult miceGTPase Rac1Skin-resident immune cellsNon-random spatial distributionDistribution of LCContinuous turnoverEpidermal cellsEpidermal T cellsIndividual cellsCellular mechanismsEnvironmental insultsHomeostasisBasal epithelial cellsImmune cellsT cellsAdult miceCellsProper architectureEpidermis
2019
Hair follicle regeneration suppresses Ras-driven oncogenic growth
Pineda CM, Gonzalez DG, Matte-Martone C, Boucher J, Lathrop E, Gallini S, Fons NR, Xin T, Tai K, Marsh E, Nguyen DX, Suozzi KC, Beronja S, Greco V. Hair follicle regeneration suppresses Ras-driven oncogenic growth. Journal Of Cell Biology 2019, 218: 3212-3222. PMID: 31488583, PMCID: PMC6781447, DOI: 10.1083/jcb.201907178.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinogenesisHair FollicleMiceMice, TransgenicNeoplasmsRas ProteinsRegenerationConceptsHair folliclesHras mutationsOncogenic growthHair follicle stem cellsSkin hair folliclesTumor developmentFollicle stem cellsHair follicle regenerationSkin epitheliumSecondary mutationsBenign outgrowthFolliclesStem cellsTissueCertain tissuesFollicle regenerationCellsContinuous tissueWild-type neighborsDistinct mechanismsDifferent outcomesMutationsEnhanced capacityInjury
2018
Flexible fate determination ensures robust differentiation in the hair follicle
Xin T, Gonzalez D, Rompolas P, Greco V. Flexible fate determination ensures robust differentiation in the hair follicle. Nature Cell Biology 2018, 20: 1361-1369. PMID: 30420661, PMCID: PMC6314017, DOI: 10.1038/s41556-018-0232-y.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineageHair FollicleHomeostasisMice, KnockoutMice, TransgenicSingle-Cell AnalysisStem CellsConceptsSingle-cell levelStem cellsStem cell differentiationGerm stem cellsTissue architectureMultiple cell typesFate determinationDetermination mechanismTissue homeostasisSame stem cellsCommon progenitorDifferentiation outcomesDifferentiation stimuliDifferentiation lineageCell differentiationCell typesNormal differentiationWnt activationHair folliclesUnanticipated flexibilityDifferentiationRobust differentiationProgenitorsCellsUninjured conditionPositional Stability and Membrane Occupancy Define Skin Fibroblast Homeostasis In Vivo
Marsh E, Gonzalez DG, Lathrop EA, Boucher J, Greco V. Positional Stability and Membrane Occupancy Define Skin Fibroblast Homeostasis In Vivo. Cell 2018, 175: 1620-1633.e13. PMID: 30415836, PMCID: PMC7605015, DOI: 10.1016/j.cell.2018.10.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCell NucleusCells, CulturedFibroblastsHomeostasisMiceMice, TransgenicSkinConceptsCore cellular mechanismsFibroblast homeostasisRac1-dependent mannerNeighboring fibroblastsMembrane occupancyMembrane extensionsNucleus organizationCellular mechanismsHomeostasisFibroblast membranesAbsence of migrationFibroblastsCell occupancySkin fibroblastsProgressive accumulationVivoStructural componentsLifespan of miceLive miceOccupancyMicePositional stabilityMembraneLifespanAccumulation
2016
Spatiotemporal coordination of stem cell commitment during epidermal homeostasis
Rompolas P, Mesa KR, Kawaguchi K, Park S, Gonzalez D, Brown S, Boucher J, Klein AM, Greco V. Spatiotemporal coordination of stem cell commitment during epidermal homeostasis. Science 2016, 352: 1471-1474. PMID: 27229141, PMCID: PMC4958018, DOI: 10.1126/science.aaf7012.Peer-Reviewed Original Research
2015
Intravital imaging of hair follicle regeneration in the mouse
Pineda CM, Park S, Mesa KR, Wolfel M, Gonzalez DG, Haberman AM, Rompolas P, Greco V. Intravital imaging of hair follicle regeneration in the mouse. Nature Protocols 2015, 10: 1116-1130. PMID: 26110716, PMCID: PMC4632978, DOI: 10.1038/nprot.2015.070.Peer-Reviewed Original Research
2012
Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration
Rompolas P, Deschene ER, Zito G, Gonzalez DG, Saotome I, Haberman AM, Greco V. Live imaging of stem cell and progeny behaviour in physiological hair-follicle regeneration. Nature 2012, 487: 496-499. PMID: 22763436, PMCID: PMC3772651, DOI: 10.1038/nature11218.Peer-Reviewed Original Research
2009
A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration
Greco V, Chen T, Rendl M, Schober M, Pasolli HA, Stokes N, dela Cruz-Racelis J, Fuchs E. A Two-Step Mechanism for Stem Cell Activation during Hair Regeneration. Cell Stem Cell 2009, 4: 155-169. PMID: 19200804, PMCID: PMC2668200, DOI: 10.1016/j.stem.2008.12.009.Peer-Reviewed Original ResearchConceptsBMP inhibitorsHair germHG cellsStem cellsStem cell activationHair regenerationTransit-amplifying cellsBulge stem cellsDermal papillaCyclic boutsTranscriptional profilingElevated FGFPrecocious activityHair folliclesBulge cellsLate telogenWntCell clustersCell activationCellsTwo-step mechanismSmall cell clustersInitial stepRegenerationInhibitors
2003
Defining the Epithelial Stem Cell Niche in Skin
Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E. Defining the Epithelial Stem Cell Niche in Skin. Science 2003, 303: 359-363. PMID: 14671312, PMCID: PMC2405920, DOI: 10.1126/science.1092436.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CycleCell DivisionCell SeparationEpidermal CellsEpidermisEpithelial CellsGene Expression ProfilingGene Expression RegulationGreen Fluorescent ProteinsHair FollicleHistonesKeratinocytesLuminescent ProteinsMiceMice, TransgenicMicroscopy, FluorescenceMultipotent Stem CellsOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTranscription, GeneticConceptsStem cell nicheLabel-retaining cellsCell nicheSkin stem cell nichesCell type-specific fashionType-specific fashionEpithelial stem cell nicheTranscriptional profilesSlow-cycling cellsHigh proliferative capacityMessenger RNANicheSurface receptorsProliferative capacityRegenerative cellsCellsRNAProgenyProtein