2011
Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies
Lim H, Han J, Woo D, Kim S, Kim S, Kang H, Kim J. Biochemical and Morphological Effects of Hypoxic Environment on Human Embryonic Stem Cells in Long-Term Culture and Differentiating Embryoid Bodies. Molecules And Cells 2011, 31: 123-132. PMID: 21347709, PMCID: PMC3932683, DOI: 10.1007/s10059-011-0016-8.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsMild hypoxiaEmbryonic stem cellsEmbryoid bodiesLong-term cultureBrdU incorporationCell fate determinationDifferentiating embryoid bodiesExpression of markersCaspase-3 immunostainingStem cellsPercentage of cellsEmbryonic germ layersActive caspase-3Hematoxylineosin stainingMammalian reproductive tract
2006
Involvement of the Fas–Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell–only syndrome
Kim S, Yoon Y, Park Y, Seo J, Kim J. Involvement of the Fas–Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell–only syndrome. Fertility And Sterility 2006, 87: 547-553. PMID: 17123522, DOI: 10.1016/j.fertnstert.2006.07.1524.Peer-Reviewed Original ResearchConceptsMaturation arrestActive caspase-3Sertoli cellsCaspase-3Testicular expressionDUTP nick end labeling stainingCases of SCOTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingNick end labeling stainingTransferase-mediated dUTP nick-end labeling stainingTesticular biopsy specimensTestes of patientsGerm cellsTranscription-polymerase chain reactionFas-Fas ligand systemRoutine clinical purposesHyperplastic interstitial cellsExpression of FasLExpression of FasBiopsy specimensMAIN OUTCOMEObstructive azoospermiaImmunohistochemical analysisIntense immunostainingLabeling staining