2019
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
Howell M, Dumitrescu DG, Blankenship LR, Herkert D, Hatzios SK. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae. Journal Of Biological Chemistry 2019, 294: 9888-9900. PMID: 31076508, PMCID: PMC6597830, DOI: 10.1074/jbc.ra119.007745.Peer-Reviewed Original ResearchConceptsSubtilisin-like enzymesSerine proteasesSubtilisin-like serine proteaseProtein domain structureDiarrheal disease choleraSerine hydrolase activityNutrient acquisitionAquatic nichesCarbohydrate-binding proteinsPathogen enzymesMature enzymeReporter proteinBiofilm assemblyFunctional characterizationNumerous enzymesDisease choleraCleavage eventsIntelectinLarge familyStepwise processingVibrio choleraeCleavage patternsEnzymeProteaseHydrolase activity
2016
Chemoproteomic profiling of host and pathogen enzymes active in cholera
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK. Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature Chemical Biology 2016, 12: 268-274. PMID: 26900865, PMCID: PMC4765928, DOI: 10.1038/nchembio.2025.Peer-Reviewed Original Research
2014
Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4
Munera D, Ritchie JM, Hatzios SK, Bronson R, Fang G, Schadt EE, Davis BM, Waldor MK. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nature Communications 2014, 5: 3080. PMID: 24445323, PMCID: PMC3905246, DOI: 10.1038/ncomms4080.Peer-Reviewed Original ResearchConceptsEnteroaggregative Escherichia coliIntestinal colonizationAggregative adherenceHaemolytic uraemic syndromeShiga toxin-producing Escherichia coli O104Outbreak of diarrheaIntestinal pathologyUraemic syndromeEscherichia coli O104Diarrhoeal diseaseOutbreak strainShiga toxinDiarrheaColi O104Subset of strainsAdherenceRobust colonizationCultured cellsCharacteristic patternVirulenceSyndromeDiseasePathologyColonization