2022
A propeptide-based biosensor for the selective detection of Vibrio cholerae using an environment-sensitive fluorophore
DeColli AA, Koolik IM, Seminara AB, Hatzios SK. A propeptide-based biosensor for the selective detection of Vibrio cholerae using an environment-sensitive fluorophore. Cell Chemical Biology 2022, 29: 1505-1516.e7. PMID: 36270233, DOI: 10.1016/j.chembiol.2022.09.003.Peer-Reviewed Original ResearchMeSH KeywordsBiosensing TechniquesFluorescent DyesPeptide HydrolasesProtease InhibitorsVibrio cholerae
2019
Functional characterization of a subtilisin-like serine protease from Vibrio cholerae
Howell M, Dumitrescu DG, Blankenship LR, Herkert D, Hatzios SK. Functional characterization of a subtilisin-like serine protease from Vibrio cholerae. Journal Of Biological Chemistry 2019, 294: 9888-9900. PMID: 31076508, PMCID: PMC6597830, DOI: 10.1074/jbc.ra119.007745.Peer-Reviewed Original ResearchConceptsSubtilisin-like enzymesSerine proteasesSubtilisin-like serine proteaseProtein domain structureDiarrheal disease choleraSerine hydrolase activityNutrient acquisitionAquatic nichesCarbohydrate-binding proteinsPathogen enzymesMature enzymeReporter proteinBiofilm assemblyFunctional characterizationNumerous enzymesDisease choleraCleavage eventsIntelectinLarge familyStepwise processingVibrio choleraeCleavage patternsEnzymeProteaseHydrolase activity
2016
Chemoproteomic profiling of host and pathogen enzymes active in cholera
Hatzios SK, Abel S, Martell J, Hubbard T, Sasabe J, Munera D, Clark L, Bachovchin DA, Qadri F, Ryan ET, Davis BM, Weerapana E, Waldor MK. Chemoproteomic profiling of host and pathogen enzymes active in cholera. Nature Chemical Biology 2016, 12: 268-274. PMID: 26900865, PMCID: PMC4765928, DOI: 10.1038/nchembio.2025.Peer-Reviewed Original Research
2012
Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition
Hatzios SK, Ringgaard S, Davis BM, Waldor MK. Studies of Dynamic Protein-Protein Interactions in Bacteria Using Renilla Luciferase Complementation Are Undermined by Nonspecific Enzyme Inhibition. PLOS ONE 2012, 7: e43175. PMID: 22905225, PMCID: PMC3419657, DOI: 10.1371/journal.pone.0043175.Peer-Reviewed Original ResearchConceptsDynamic protein-protein interactionsProtein-protein interactionsLuciferase complementationFull-length luciferaseLive eukaryotic cellsEnteric pathogen Vibrio choleraeProtein fragment complementationSmall molecule effectsPathogen Vibrio choleraeEnzyme activityPhosphatase CheZEukaryotic cellsFragment complementationChemotaxis mutantsLuciferase fragmentsChemotactic signalingBacterial chemotaxisFusion proteinComplementationRenilla luciferaseLuciferase activityCheY3Vibrio choleraeProteinLuciferase