2006
Synthesis of a Biotin Derivative of Iberiotoxin: Binding Interactions with Streptavidin and the BK Ca2+-Activated K+ Channel Expressed in a Human Cell Line
Bingham J, Bian S, Tan Z, Takacs Z, Moczydlowski E. Synthesis of a Biotin Derivative of Iberiotoxin: Binding Interactions with Streptavidin and the BK Ca2+-Activated K+ Channel Expressed in a Human Cell Line. Bioconjugate Chemistry 2006, 17: 689-699. PMID: 16704206, PMCID: PMC2505059, DOI: 10.1021/bc060002u.Peer-Reviewed Original ResearchThe calcium-sensitive large-conductance potassium channel (BK/MAXI K) is present in the inner mitochondrial membrane of rat brain
Douglas R, Lai J, Bian S, Cummins L, Moczydlowski E, Haddad G. The calcium-sensitive large-conductance potassium channel (BK/MAXI K) is present in the inner mitochondrial membrane of rat brain. Neuroscience 2006, 139: 1249-1261. PMID: 16567053, DOI: 10.1016/j.neuroscience.2006.01.061.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternBrainCitrate (si)-SynthaseImmunohistochemistryLarge-Conductance Calcium-Activated Potassium ChannelsMembrane ProteinsMicroscopy, Electron, TransmissionMitochondrial MembranesRatsRats, Sprague-DawleySubcellular FractionsConceptsLarge conductance voltageInner mitochondrial membraneCalcium-sensitive channelsCytochrome c oxidaseMitochondrial membraneInner mitochondrial membrane proteinC oxidaseMitochondrial fractionInner membrane 23Mitochondrial-specific proteinsMitochondrial membrane proteinMicrotubule-associated protein 1Endoplasmic reticulum markerImmuno-gold electron microscopyLarge-conductance potassium channelsDiscrete subcellular fractionsGold particle labellingWestern blottingGolgi proteinsMembrane proteinsSubcellular localizationAlpha subunitProtein 1Mitochondrial pelletMitochondria
2001
Ca2+-binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca2+-dependent activation
Bian S, Favre I, Moczydlowski E. Ca2+-binding activity of a COOH-terminal fragment of the Drosophila BK channel involved in Ca2+-dependent activation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 4776-4781. PMID: 11274367, PMCID: PMC31910, DOI: 10.1073/pnas.081072398.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumCell LineDrosophilaDrosophila ProteinsHumansIon Channel GatingLarge-Conductance Calcium-Activated Potassium ChannelsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Calcium-ActivatedProtein BindingConceptsIntracellular COOH-terminal domainDependent activationBK channelsCOOH-terminal domainProtein blot assayBiochemical approachesStructural basisBiophysical analysisBK proteinAsp residuesControl proteinHEK293 cellsEscherichia coliProteinElectrophysiological assaysBlot assaysLarge conductanceTerminal fragmentSoluble formActivationResiduesFunctional correlationSpecific bindingSpecific regionsBinding