2024
Acyl-CoA Synthetase Medium-Chain Family Member 5-Mediated Fatty Acid Metabolism Dysregulation Promotes the Progression of Hepatocellular Carcinoma.
Yang L, Pham K, Xi Y, Jiang S, Robertson KD, Liu C. Acyl-CoA Synthetase Medium-Chain Family Member 5-Mediated Fatty Acid Metabolism Dysregulation Promotes the Progression of Hepatocellular Carcinoma. Am J Pathol 2024, 194: 1951-1966. PMID: 39069168, DOI: 10.1016/j.ajpath.2024.07.002.Peer-Reviewed Original Research
2023
1180-P: Impact of Diabetes Exposure In Utero on Mesenchymal Stem Cell Phenotype and Metabolic Function
HAN LEE W, REDLING D, BROOKS O, TEAGUE A, JIANG S, TRYGGESTAD J. 1180-P: Impact of Diabetes Exposure In Utero on Mesenchymal Stem Cell Phenotype and Metabolic Function. Diabetes 2023, 72 DOI: 10.2337/db23-1180-p.Peer-Reviewed Original ResearchControl MSCsExposure to diabetesUmbilical cord Wharton's jellyStem cell phenotypeAbsence of CD34Fetal programming eventsIsotype-control antibodyPresence of CD105Maximal respirationMesenchymal stem cell phenotypeCapacity of MSCsDiabetes exposureCharacteristics of MSCsFetal tissuesUteroWharton's jellyDiabetic milieuExposure to DMCell phenotypeMSC abilityPassage 4Adipogenic capacityEnergy expenditureMetabolic characteristicsCD13AMPK Regulates DNA Methylation of PGC-1α and Myogenic Differentiation in Human Mesenchymal Stem Cells
Wu J, Gulati S, Teague A, Kim Y, Tryggestad J, Jiang S. AMPK Regulates DNA Methylation of PGC-1α and Myogenic Differentiation in Human Mesenchymal Stem Cells. Stem Cells And Development 2023, 32: 131-139. PMID: 36594575, PMCID: PMC9986020, DOI: 10.1089/scd.2022.0226.Peer-Reviewed Original ResearchConceptsActivation of AMP-activated protein kinasePGC-1a expressionMitochondrial programDNA methylationCompound CMitochondrial biogenesisAMPK activationActivation of AMPKAMP-activated protein kinaseAMPK inhibitor compound CInhibition of DNA methylationRegulator of mitochondrial biogenesisRegulate mitochondrial biogenesisPGC-1aInhibitor compound CPromoter methylationIncreased mitochondrial functionRegulation of energy metabolismMitochondrial DNAEpigenetic landscapeDNA demethylationMtDNA abundanceMyogenic differentiationEpigenetic regulationProtein kinase
2022
miR-130b/301b Is a Negative Regulator of Beige Adipogenesis and Energy Metabolism In Vitro and In Vivo.
Luo W, Kim Y, Jensen M, Herlea-Pana O, Wang W, Rudolph M, Friedman J, Chernausek S, Jiang S. miR-130b/301b Is a Negative Regulator of Beige Adipogenesis and Energy Metabolism In Vitro and In Vivo. Diabetes 2022, 71: 2360-2371. PMID: 36001751, PMCID: PMC9630090, DOI: 10.2337/db22-0205.Peer-Reviewed Original ResearchConceptsBeige adipogenesisMiR-301bMiR-130bPeroxisome proliferator-activated receptor γ coactivator 1αProliferator-activated receptor γ coactivator 1αImproved glucose toleranceReceptor γ coactivator 1αLess weight gainPotential therapeutic targetCold-induced energy expenditureΓ coactivator 1αMitochondrial biogenesisMetabolic complicationsVisceral adiposityGlucose toleranceThermogenic brownCounteract obesityMetabolic disordersTherapeutic targetAdipose tissueBeige phenotypeMetabolic diseasesAdipose progenitor cellsBeige adipocytesCoactivator 1α
2021
AMPK activates Parkin independent autophagy and improves post sepsis immune defense against secondary bacterial lung infections.
Bone NB, Becker EJ Jr, Husain M, Jiang S, Zmijewska AA, Park DW, Chacko B, Darley-Usmar V, Grégoire M, Tadie JM, Thannickal VJ, Zmijewski JW. AMPK activates Parkin independent autophagy and improves post sepsis immune defense against secondary bacterial lung infections. Sci Rep 2021, 11: 12387. PMID: 34117280, DOI: 10.1038/s41598-021-90573-0.Peer-Reviewed Original Research1168-P: Serum MicroRNA-122 and -192 Are Increased in Adolescents with NAFLD: Potential Role as Biomarkers
SHORT K, PALLE S, HELLMAN D, JIANG S, TRYGGESTAD J, GARCIA E. 1168-P: Serum MicroRNA-122 and -192 Are Increased in Adolescents with NAFLD: Potential Role as Biomarkers. Diabetes 2021, 70 DOI: 10.2337/db21-1168-p.Peer-Reviewed Original ResearchMiR-122MiR-130bMiR-155MicroRNA-122Abundance of miR-122Serum miR-122Serum microRNA-122Serum miR-155MiR-192Nonalcoholic fatty liver diseaseNonalcoholic fatty liver disease patientsNonalcoholic fatty liver disease groupAlanine aminotransferaseLiver biopsyBiopsy-confirmed nonalcoholic fatty liver disease
2020
Fetal circulating human resistin increases in diabetes during pregnancy and impairs placental mitochondrial biogenesis
Jiang S, Teague A, Tryggestad J, Lyons T, Chernausek S. Fetal circulating human resistin increases in diabetes during pregnancy and impairs placental mitochondrial biogenesis. Molecular Medicine 2020, 26: 76. PMID: 32762639, PMCID: PMC7409642, DOI: 10.1186/s10020-020-00205-y.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAdultBiomarkersBlood GlucoseCase-Control StudiesDiabetes, GestationalDNA, MitochondrialFemaleFetal BloodHumansLeukocytes, MononuclearMaternal ExposureMesenchymal Stem CellsMitochondriaOrganelle BiogenesisPlacentaPregnancyPrenatal Exposure Delayed EffectsResistinStress, PhysiologicalTrophoblastsConceptsCord blood mononuclear cellsBlood mononuclear cellsSecretion of resistinHuman resistinMononuclear cellsBeWo cellsCultured cord blood mononuclear cellsMeasures of maternal glycemiaMesenchymal stem cellsLong-term health of offspringExposed BeWo cellsImpact fetal developmentPlacental mitochondrial contentCirculation of infantsPlacental trophoblast cellsHuman placental explantsAdipocyte-derived factorsHealth of offspringMaternal glycemiaResponse to high glucoseDiabetic pregnanciesFetal circulationMaternal diabetesCord bloodCord seraRole of metformin in epigenetic regulation of placental mitochondrial biogenesis in maternal diabetes
Jiang S, Teague A, Tryggestad J, Jensen M, Chernausek S. Role of metformin in epigenetic regulation of placental mitochondrial biogenesis in maternal diabetes. Scientific Reports 2020, 10: 8314. PMID: 32433500, PMCID: PMC7239922, DOI: 10.1038/s41598-020-65415-0.Peer-Reviewed Original ResearchMeSH KeywordsAcetylationAdultAMP-Activated Protein KinasesAnimalsCohort StudiesDiabetes, GestationalDiet, High-FatDNA MethylationEpigenesis, GeneticFemaleHistonesHumansMaleMetforminMiceMice, Inbred C57BLMitochondriaOrganelle BiogenesisPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPlacentaPregnancyPregnancy in DiabeticsPromoter Regions, GeneticSex FactorsConceptsActivation of AMP-activated protein kinaseMitochondrial biogenesisPGC-1a expressionH3K27 acetylationAMP-activated protein kinaseRegulator of mitochondrial biogenesisMitochondrial transcription factor ASubgroup of diabetic womenAssociated with epigenetic regulationPGC-1aAberrant epigenetic alterationsLevels of H3K27 acetylationOffspring long-term healthProliferator-activated receptor gamma coactivator 1-alphaPeroxisome proliferator-activated receptor gamma coactivator 1-alphaPromoter methylationTranscription factor AImpaired mitochondrial biogenesisIncreased promoter methylationGamma coactivator 1-alphaDecreased histone acetylationIncreased H3K27 acetylationCoactivator 1-alphaLong-term healthCapability of metforminRole of miR‐130b/301b cluster in macrophage polarization and obesity
Jiang S, McBride A, Luo W, Chernausek S. Role of miR‐130b/301b cluster in macrophage polarization and obesity. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.09682.Peer-Reviewed Original ResearchDevelopment of obesityMacrophage polarizationKnockout miceMacrophage M1/M2 polarizationM1/M2 polarizationPro-inflammatory activationWhole-body energy expenditureHigh-fat dietEnergy expenditureAlternative macrophage activationWild-type miceWhite adipose tissueBody energy expenditureMacrophage activation statusBone marrow macrophagesPresent studyFat dietIL-4M2 polarizationType miceAlternative activationMacrophage activationAdipose tissueMetabolic diseasesActivation status
2019
Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma
Tryggestad J, Teague A, Sparling D, Jiang S, Chernausek S. Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma. Obesity 2019, 27: 1856-1864. PMID: 31531958, PMCID: PMC6832842, DOI: 10.1002/oby.22616.Peer-Reviewed Original ResearchConceptsAdipose tissue macrophagesMiR-155Mesenchymal stem cellsStem cellsPeripheral blood mononuclear cellsPeroxisome proliferator-activated receptor gammaBlood mononuclear cellsImpact of obesityProliferator-activated receptor gammaInfluence adipocyte metabolismMiR-130b expressionCell type-specific fashionIncreased 1.8-foldType-specific fashionPPARG mRNA expressionWestern blot analysisMononuclear cellsPolymerase chain reactionQuantitative polymerase chain reactionMiRNA expressionHuman mesenchymal stem cellsTissue macrophagesDifferentiated 3T3-L1 adipocytesMRNA expressionObesity
2018
Metformin reverses established lung fibrosis in a bleomycin model.
Rangarajan S, Bone NB, Zmijewska AA, Jiang S, Park DW, Bernard K, Locy ML, Ravi S, Deshane J, Mannon RB, Abraham E, Darley-Usmar V, Thannickal VJ, Zmijewski JW. Metformin reverses established lung fibrosis in a bleomycin model. Nat Med 2018, 24: 1121-1127. PMID: 29967351, DOI: 10.1038/s41591-018-0087-6.Peer-Reviewed Original ResearchRegulation and Role of Human Resistin in Diabetes during Pregnancy
JIANG S, TEAGUE A, TRYGGESTAD J, LYONS T, CHERNAUSEK S. Regulation and Role of Human Resistin in Diabetes during Pregnancy. Diabetes 2018, 67 DOI: 10.2337/db18-1401-p.Peer-Reviewed Original ResearchCord blood mononuclear cellsDiabetic pregnanciesHuman resistinCultured cord blood mononuclear cellsMeasures of maternal glycemiaEffects of maternal diabetesLong-term health of offspringImpact fetal developmentOffspring of diabetic pregnancyPlacental trophoblast cellsBlood mononuclear cellsSecretion of resistinHuman term placentaAdipocyte-derived factorsHealth of offspringMaternal glycemiaResponse to high glucoseFetal circulationMaternal diabetesPlacental functionCord seraFetal developmentResistin levelsTerm placentaResistin concentrations
2017
Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis
Jiang S, Teague A, Tryggestad J, Aston C, Lyons T, Chernausek S. Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis. Placenta 2017, 57: 26-32. PMID: 28864016, PMCID: PMC5659203, DOI: 10.1016/j.placenta.2017.06.001.Peer-Reviewed Original ResearchConceptsMtDNA contentResponse to maternal diabetesMitochondrial biogenesisMitochondrial DNAPGC-1aEffects of maternal diabetesMaternal diabetesRegulator of mitochondrial biogenesisTranscription factor AImpaired mitochondrial biogenesisMitochondria transcription factor ABiogenesis pathwayMetabolic diseasesMtDNAFetal sex-specific differencesCopy numberTFAM levelsGene expressionMale offspringBiogenesisAbnormal placental functionTFAMNon-diabetic womenOffspring sexIndex of mitochondrial biogenesisRole of microRNA-130b in placental PGC-1α/TFAM mitochondrial biogenesis pathway
Jiang S, Teague A, Tryggestad J, Chernausek S. Role of microRNA-130b in placental PGC-1α/TFAM mitochondrial biogenesis pathway. Biochemical And Biophysical Research Communications 2017, 487: 607-612. PMID: 28433632, PMCID: PMC5522006, DOI: 10.1016/j.bbrc.2017.04.099.Peer-Reviewed Original ResearchConceptsMiR-130b-3pPGC-1a expressionPGC-1aReactive oxygen speciesMicroRNA-130bExpression of miR-130b-3pRegulator of mitochondrial biogenesisMitochondrial transcription factor ADecreased protein abundanceTranscription factor AOffspring long-term healthResponse to high glucoseCells to high glucoseHigh glucoseMitochondrial biogenesis signalingLong-term healthOxidative stressMitochondrial biogenesisMitochondrial functionBeWo cellsTFAM expressionBiogenesis signalingMolecular mechanismsDownstream factorsHigh-glucose treatment
2016
Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA.
Tryggestad J, Vishwanath A, Jiang S, Mallappa A, Teague A, Takahashi Y, Thompson D, Chernausek S. Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA. Clinical Science 2016, 130: 1955-67. PMID: 27562513, PMCID: PMC5516927, DOI: 10.1042/cs20160305.Peer-Reviewed Original ResearchConceptsGestational diabetes mellitusMiR-130bMiR-let-7a-5pMiR-148aLevel of miR-130bHuman umbilical vein endothelial cellsMiR-148a-3pMiR-30c-5pMiR-148a mimicsInfants of womenMiR-130b-3pMiRNA expressionMiR-452MiR-126Influence of gestational diabetes mellitusDiabetes mellitusTarget protein expressionProtein expressionTarget protein abundanceMiRNAsMonths of ageUmbilical vein endothelial cellsMiRNA speciesQuantitative PCRFetal tissuesAMP-Activated Protein Kinase and Glycogen Synthase Kinase 3β Modulate the Severity of Sepsis-Induced Lung Injury.
Liu Z, Bone N, Jiang S, Park DW, Tadie JM, Deshane J, Rodriguez CA, Pittet JF, Abraham E, Zmijewski JW. AMP-Activated Protein Kinase and Glycogen Synthase Kinase 3β Modulate the Severity of Sepsis-Induced Lung Injury. Mol Med 2016, 21: 937-950. PMID: 26650187, DOI: 10.2119/molmed.2015.00198.Peer-Reviewed Original Research
2015
Participation of proteasome-ubiquitin protein degradation in autophagy and the activation of AMP-activated protein kinase.
Jiang S, Park DW, Gao Y, Ravi S, Darley-Usmar V, Abraham E, Zmijewski JW. Participation of proteasome-ubiquitin protein degradation in autophagy and the activation of AMP-activated protein kinase. Cell Signal 2015, 27: 1186-97. PMID: 25728513, DOI: 10.1016/j.cellsig.2015.02.024.Peer-Reviewed Original Research
2014
GSK3β-dependent inhibition of AMPK potentiates activation of neutrophils and macrophages and enhances severity of acute lung injury.
Park DW, Jiang S, Liu Y, Siegal GP, Inoki K, Abraham E, Zmijewski JW. GSK3β-dependent inhibition of AMPK potentiates activation of neutrophils and macrophages and enhances severity of acute lung injury. Am J Physiol Lung Cell Mol Physiol 2014, 307: L735-45. PMID: 25239914, DOI: 10.1152/ajplung.00165.2014.Peer-Reviewed Original ResearchHuman resistin promotes neutrophil proinflammatory activation and neutrophil extracellular trap formation and increases severity of acute lung injury.
Jiang S, Park DW, Tadie JM, Gregoire M, Deshane J, Pittet JF, Abraham E, Zmijewski JW. Human resistin promotes neutrophil proinflammatory activation and neutrophil extracellular trap formation and increases severity of acute lung injury. J Immunol 2014, 192: 4795-803. PMID: 24719460, DOI: 10.4049/jimmunol.1302764.Peer-Reviewed Original ResearchRegulation of hepatic insulin receptor activity following injury.
Jiang S, Gavrikova TA, Messina JL. Regulation of hepatic insulin receptor activity following injury. Am J Physiol Gastrointest Liver Physiol 2014, 306: G886-92. PMID: 24699331, DOI: 10.1152/ajpgi.00128.2013.Peer-Reviewed Original Research