2020
Fetal circulating human resistin increases in diabetes during pregnancy and impairs placental mitochondrial biogenesis
Jiang S, Teague A, Tryggestad J, Lyons T, Chernausek S. Fetal circulating human resistin increases in diabetes during pregnancy and impairs placental mitochondrial biogenesis. Molecular Medicine 2020, 26: 76. PMID: 32762639, PMCID: PMC7409642, DOI: 10.1186/s10020-020-00205-y.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAdultBiomarkersBlood GlucoseCase-Control StudiesDiabetes, GestationalDNA, MitochondrialFemaleFetal BloodHumansLeukocytes, MononuclearMaternal ExposureMesenchymal Stem CellsMitochondriaOrganelle BiogenesisPlacentaPregnancyPrenatal Exposure Delayed EffectsResistinStress, PhysiologicalTrophoblastsConceptsCord blood mononuclear cellsBlood mononuclear cellsSecretion of resistinHuman resistinMononuclear cellsBeWo cellsCultured cord blood mononuclear cellsMeasures of maternal glycemiaMesenchymal stem cellsLong-term health of offspringExposed BeWo cellsImpact fetal developmentPlacental mitochondrial contentCirculation of infantsPlacental trophoblast cellsHuman placental explantsAdipocyte-derived factorsHealth of offspringMaternal glycemiaResponse to high glucoseDiabetic pregnanciesFetal circulationMaternal diabetesCord bloodCord seraRole of metformin in epigenetic regulation of placental mitochondrial biogenesis in maternal diabetes
Jiang S, Teague A, Tryggestad J, Jensen M, Chernausek S. Role of metformin in epigenetic regulation of placental mitochondrial biogenesis in maternal diabetes. Scientific Reports 2020, 10: 8314. PMID: 32433500, PMCID: PMC7239922, DOI: 10.1038/s41598-020-65415-0.Peer-Reviewed Original ResearchMeSH KeywordsAcetylationAdultAMP-Activated Protein KinasesAnimalsCohort StudiesDiabetes, GestationalDiet, High-FatDNA MethylationEpigenesis, GeneticFemaleHistonesHumansMaleMetforminMiceMice, Inbred C57BLMitochondriaOrganelle BiogenesisPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPlacentaPregnancyPregnancy in DiabeticsPromoter Regions, GeneticSex FactorsConceptsActivation of AMP-activated protein kinaseMitochondrial biogenesisPGC-1a expressionH3K27 acetylationAMP-activated protein kinaseRegulator of mitochondrial biogenesisMitochondrial transcription factor ASubgroup of diabetic womenAssociated with epigenetic regulationPGC-1aAberrant epigenetic alterationsLevels of H3K27 acetylationOffspring long-term healthProliferator-activated receptor gamma coactivator 1-alphaPeroxisome proliferator-activated receptor gamma coactivator 1-alphaPromoter methylationTranscription factor AImpaired mitochondrial biogenesisIncreased promoter methylationGamma coactivator 1-alphaDecreased histone acetylationIncreased H3K27 acetylationCoactivator 1-alphaLong-term healthCapability of metformin
2019
Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma
Tryggestad J, Teague A, Sparling D, Jiang S, Chernausek S. Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma. Obesity 2019, 27: 1856-1864. PMID: 31531958, PMCID: PMC6832842, DOI: 10.1002/oby.22616.Peer-Reviewed Original ResearchConceptsAdipose tissue macrophagesMiR-155Mesenchymal stem cellsStem cellsPeripheral blood mononuclear cellsPeroxisome proliferator-activated receptor gammaBlood mononuclear cellsImpact of obesityProliferator-activated receptor gammaInfluence adipocyte metabolismMiR-130b expressionCell type-specific fashionIncreased 1.8-foldType-specific fashionPPARG mRNA expressionWestern blot analysisMononuclear cellsPolymerase chain reactionQuantitative polymerase chain reactionMiRNA expressionHuman mesenchymal stem cellsTissue macrophagesDifferentiated 3T3-L1 adipocytesMRNA expressionObesity
2017
Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis
Jiang S, Teague A, Tryggestad J, Aston C, Lyons T, Chernausek S. Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis. Placenta 2017, 57: 26-32. PMID: 28864016, PMCID: PMC5659203, DOI: 10.1016/j.placenta.2017.06.001.Peer-Reviewed Original ResearchConceptsMtDNA contentResponse to maternal diabetesMitochondrial biogenesisMitochondrial DNAPGC-1aEffects of maternal diabetesMaternal diabetesRegulator of mitochondrial biogenesisTranscription factor AImpaired mitochondrial biogenesisMitochondria transcription factor ABiogenesis pathwayMetabolic diseasesMtDNAFetal sex-specific differencesCopy numberTFAM levelsGene expressionMale offspringBiogenesisAbnormal placental functionTFAMNon-diabetic womenOffspring sexIndex of mitochondrial biogenesis
2016
Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA.
Tryggestad J, Vishwanath A, Jiang S, Mallappa A, Teague A, Takahashi Y, Thompson D, Chernausek S. Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA. Clinical Science 2016, 130: 1955-67. PMID: 27562513, PMCID: PMC5516927, DOI: 10.1042/cs20160305.Peer-Reviewed Original ResearchConceptsGestational diabetes mellitusMiR-130bMiR-let-7a-5pMiR-148aLevel of miR-130bHuman umbilical vein endothelial cellsMiR-148a-3pMiR-30c-5pMiR-148a mimicsInfants of womenMiR-130b-3pMiRNA expressionMiR-452MiR-126Influence of gestational diabetes mellitusDiabetes mellitusTarget protein expressionProtein expressionTarget protein abundanceMiRNAsMonths of ageUmbilical vein endothelial cellsMiRNA speciesQuantitative PCRFetal tissues