2019
Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma
Tryggestad J, Teague A, Sparling D, Jiang S, Chernausek S. Macrophage‐Derived microRNA‐155 Increases in Obesity and Influences Adipocyte Metabolism by Targeting Peroxisome Proliferator‐Activated Receptor Gamma. Obesity 2019, 27: 1856-1864. PMID: 31531958, PMCID: PMC6832842, DOI: 10.1002/oby.22616.Peer-Reviewed Original ResearchConceptsAdipose tissue macrophagesMiR-155Mesenchymal stem cellsStem cellsPeripheral blood mononuclear cellsPeroxisome proliferator-activated receptor gammaBlood mononuclear cellsImpact of obesityProliferator-activated receptor gammaInfluence adipocyte metabolismMiR-130b expressionCell type-specific fashionIncreased 1.8-foldType-specific fashionPPARG mRNA expressionWestern blot analysisMononuclear cellsPolymerase chain reactionQuantitative polymerase chain reactionMiRNA expressionHuman mesenchymal stem cellsTissue macrophagesDifferentiated 3T3-L1 adipocytesMRNA expressionObesity
2017
Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis
Jiang S, Teague A, Tryggestad J, Aston C, Lyons T, Chernausek S. Effects of maternal diabetes and fetal sex on human placenta mitochondrial biogenesis. Placenta 2017, 57: 26-32. PMID: 28864016, PMCID: PMC5659203, DOI: 10.1016/j.placenta.2017.06.001.Peer-Reviewed Original ResearchConceptsMtDNA contentResponse to maternal diabetesMitochondrial biogenesisMitochondrial DNAPGC-1aEffects of maternal diabetesMaternal diabetesRegulator of mitochondrial biogenesisTranscription factor AImpaired mitochondrial biogenesisMitochondria transcription factor ABiogenesis pathwayMetabolic diseasesMtDNAFetal sex-specific differencesCopy numberTFAM levelsGene expressionMale offspringBiogenesisAbnormal placental functionTFAMNon-diabetic womenOffspring sexIndex of mitochondrial biogenesis
2016
Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA.
Tryggestad J, Vishwanath A, Jiang S, Mallappa A, Teague A, Takahashi Y, Thompson D, Chernausek S. Influence of gestational diabetes mellitus on human umbilical vein endothelial cell miRNA. Clinical Science 2016, 130: 1955-67. PMID: 27562513, PMCID: PMC5516927, DOI: 10.1042/cs20160305.Peer-Reviewed Original ResearchConceptsGestational diabetes mellitusMiR-130bMiR-let-7a-5pMiR-148aLevel of miR-130bHuman umbilical vein endothelial cellsMiR-148a-3pMiR-30c-5pMiR-148a mimicsInfants of womenMiR-130b-3pMiRNA expressionMiR-452MiR-126Influence of gestational diabetes mellitusDiabetes mellitusTarget protein expressionProtein expressionTarget protein abundanceMiRNAsMonths of ageUmbilical vein endothelial cellsMiRNA speciesQuantitative PCRFetal tissues