2020
A New Link to Primate Heart Development
Nelakanti RV, Xiao AZ. A New Link to Primate Heart Development. Developmental Cell 2020, 54: 685-686. PMID: 32991832, DOI: 10.1016/j.devcel.2020.09.009.Commentaries, Editorials and LettersN6-methyladenine in DNA antagonizes SATB1 in early development
Li Z, Zhao S, Nelakanti RV, Lin K, Wu TP, Alderman MH, Guo C, Wang P, Zhang M, Min W, Jiang Z, Wang Y, Li H, Xiao AZ. N6-methyladenine in DNA antagonizes SATB1 in early development. Nature 2020, 583: 625-630. PMID: 32669713, PMCID: PMC8596487, DOI: 10.1038/s41586-020-2500-9.Peer-Reviewed Original ResearchConceptsN6-mAN6-methyladenineMouse trophoblast stem cellsLarge chromatin domainsCell fate transitionsLarge-scale chromatinUnexpected molecular mechanismTrophoblast stem cellsEarly embryonic developmentDNA secondary structuresEarly developmentFate transitionsMammalian genomesChromatin domainsEpigenetic landscapeGene regulationChromatin organizerEmbryonic developmentDNA modificationsBiological roleMolecular mechanismsSATB1 functionsMolecular pathwaysCell culture modelSecondary structure
2018
Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo
Kooreman NG, Kim Y, de Almeida PE, Termglinchan V, Diecke S, Shao NY, Wei TT, Yi H, Dey D, Nelakanti R, Brouwer TP, Paik DT, Sagiv-Barfi I, Han A, Quax PHA, Hamming JF, Levy R, Davis MM, Wu JC. Autologous iPSC-Based Vaccines Elicit Anti-tumor Responses In Vivo. Cell Stem Cell 2018, 22: 501-513.e7. PMID: 29456158, PMCID: PMC6134179, DOI: 10.1016/j.stem.2018.01.016.Peer-Reviewed Original ResearchConceptsElicit anti-tumor responsesAnti-tumor responseInduced pluripotent stem cellsTumor growthMetastatic tumor loadTumor-associated antigensAnti-tumor vaccinesMurine breast cancerTumor-bearing miceAutologous induced pluripotent stem cellsUnvaccinated recipientsProphylactic settingAdoptive transferClinical immunotherapyCancer vaccinesTumor loadMelanoma recurrenceBreast cancerResection siteMelanoma modelMyeloid cellsCell responsesVaccineCancer cellsCancer
2017
Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics
Kooreman NG, de Almeida PE, Stack JP, Nelakanti RV, Diecke S, Shao NY, Swijnenburg RJ, Sanchez-Freire V, Matsa E, Liu C, Connolly AJ, Hamming JF, Quax PHA, Brehm MA, Greiner DL, Shultz LD, Wu JC. Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics. Cell Reports 2017, 20: 1978-1990. PMID: 28834758, PMCID: PMC5573767, DOI: 10.1016/j.celrep.2017.08.003.Peer-Reviewed Original ResearchConceptsHuman immune responseHumanized miceImmune cellsImmune responseStem cell allograftsInnate immune cellsAllogeneic stem cellsStem cellsMouse immune systemAlloimmune responseCell allograftsImmunodeficient miceMouse modelGraft persistenceImmune systemHuman lymphocytesMiceAllograftsStem cell therapeuticsCell therapeuticsPluripotent stem cellsEmbryonic stem cellsCellsAlloimmunityResponse
2015
Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research
Nelakanti RV, Kooreman NG, Wu JC. Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research. Current Protocols In Stem Cell Biology 2015, 32: 4a.8.1-4a.8.17. PMID: 25640819, PMCID: PMC4402211, DOI: 10.1002/9780470151808.sc04a08s32.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsEmbryonic germ layersInduced pluripotent stem cellsPluripotent stem cellsSpecific tissue typesStem cell researchGerm layersPluripotencyTeratoma formationTeratoma growthStem cellsTissue typesCell researchCell linesDetailed identificationMesodermCellsImmunodeficient miceProtocolGrowthLayerIdentification