2013
Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3
Hayashi H, Tamura A, Krishnan D, Tsukita S, Suzuki Y, Kocinsky HS, Aronson PS, Orlowski J, Grinstein S, Alexander RT. Ezrin Is Required for the Functional Regulation of the Epithelial Sodium Proton Exchanger, NHE3. PLOS ONE 2013, 8: e55623. PMID: 23405179, PMCID: PMC3566197, DOI: 10.1371/journal.pone.0055623.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAnimalsColonCyclic AMPCytoskeletal ProteinsDogsEpithelial CellsFluorescence Recovery After PhotobleachingHumansMadin Darby Canine Kidney CellsMaleMembrane ProteinsMiceMice, KnockoutMicrofilament ProteinsMicrovilliOctoxynolPhosphorylationProtein TransportRNA, Small InterferingSodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersConceptsApical actin cytoskeletonNHE3 activitySodium hydrogen exchanger isoform 3CAMP-dependent inhibitionEzrin knockdown miceERM proteinsActin cytoskeletonSodium-proton exchangerApical cytoskeletonApical localizationFunctional regulationWild-type animalsEpithelial cell culture modelEzrinProton exchangersFluorescent recoveryEpithelial phenotypeCytoskeletonMolecular determinantsCell culture modelExchanger isoform 3Functional studiesNon-targeting siRNAApical membraneIsoform 3
2010
The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice
Leviel F, Hübner CA, Houillier P, Morla L, Moghrabi S, Brideau G, Hatim H, Parker MD, Kurth I, Kougioumtzes A, Sinning A, Pech V, Riemondy KA, Miller RL, Hummler E, Shull GE, Aronson PS, Doucet A, Wall SM, Chambrey R, Eladari D. The Na+-dependent chloride-bicarbonate exchanger SLC4A8 mediates an electroneutral Na+ reabsorption process in the renal cortical collecting ducts of mice. Journal Of Clinical Investigation 2010, 120: 1627-1635. PMID: 20389022, PMCID: PMC2860930, DOI: 10.1172/jci40145.Peer-Reviewed Original ResearchConceptsNa-Cl cotransporterSodium transportMaintenance of euvolemiaTransepithelial NaCl absorptionDucts of miceEpithelial sodium channelIntravascular volumeIndependent Cl-/HCO3Sodium balanceMouse CCDAmiloride-sensitive epithelial sodium channelSodium absorptionExcretion resultsFluid homeostasisGenetic ablationNaCl absorptionSodium channelsMiceCl-/HCO3NaCl transportSlc4a8Genetic disruptionNovel roleHydrochlorothiazideDuct
2007
NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity
Kocinsky HS, Dynia DW, Wang T, Aronson PS. NHE3 phosphorylation at serines 552 and 605 does not directly affect NHE3 activity. American Journal Of Physiology. Renal Physiology 2007, 293: f212-f218. PMID: 17409282, DOI: 10.1152/ajprenal.00042.2007.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAnimalsCells, CulturedColforsinCyclic AMP-Dependent Protein KinasesElectrophoresis, Polyacrylamide GelKidneyKidney Tubules, ProximalMaleMicrovilliParathyroid HormonePhosphodiesterase InhibitorsPhosphorylationRatsRats, Sprague-DawleySerineSodiumSodium RadioisotopesSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersStimulation, ChemicalConceptsSerine 552NHE3 phosphorylationNHE3 activityMicrovillar membrane vesiclesPhosphorylation of NHE3Direct phosphorylationPhosphospecific antibodiesSprague-Dawley ratsExchanger type 3PKA activationMembrane vesiclesSite-specific changesPhosphorylationTransport activityOpossum kidney cellsParathyroid hormoneIntravenous infusionRat modelExchange activityKidney cellsOKP cellsNHE3 inhibitionPKACell modelExact role
2006
Pendrin Regulation in Mouse Kidney Primarily Is Chloride-Dependent
Vallet M, Picard N, Loffing-Cueni D, Fysekidis M, Bloch-Faure M, Deschênes G, Breton S, Meneton P, Loffing J, Aronson PS, Chambrey R, Eladari D. Pendrin Regulation in Mouse Kidney Primarily Is Chloride-Dependent. Journal Of The American Society Of Nephrology 2006, 17: 2153-2163. PMID: 16825334, DOI: 10.1681/asn.2005101054.Peer-Reviewed Original ResearchConceptsHydrochlorothiazide administrationAldosterone secretionExtracellular fluid volume regulationElevated plasma aldosterone levelsPlasma aldosterone levelsDifferent mouse modelsFluid volume regulationPendrin protein expressionAldosterone levelsSecondary hyperaldosteronismEpithelial sodium channelChloride transportApical Cl-/HCO3Chloride reabsorptionRat modelPendrin expressionMouse modelKnockout miceSemiquantitative immunoblottingNaCl loadingGamma-ENaC subunitsBiologic effectsExperimental modelSodium channelsProtein expressionThe life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates
Knauf F, Mohebbi N, Teichert C, Herold D, Rogina B, Helfand S, Gollasch M, Luft FC, Aronson PS. The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates. Biochemical Journal 2006, 397: 25-29. PMID: 16608441, PMCID: PMC1479758, DOI: 10.1042/bj20060409.Peer-Reviewed Original ResearchConceptsExternal citrateKrebs cycle intermediatesXenopus oocytesSodium dicarboxylate cotransporterMammalian genesDrosophila melanogasterIndy mutantsLifespan extensionLongevity genesPlasma membraneSubstrate effluxExternal mediumSpecific transport mechanismsFunctional studiesSuccinate uptakeAddition of succinateEnergy metabolismIndyTransportable substratesGenesOocytesEffluxMelanogasterMutantsSuccinateIon exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule.
Aronson PS. Ion exchangers mediating Na+, HCO3 - and Cl- transport in the renal proximal tubule. Journal Of Nephrology 2006, 19 Suppl 9: s3-s10. PMID: 16736438.Commentaries, Editorials and LettersConceptsProximal tubulesNaCl absorptionApical membrane ClNull miceOxalate-sulfate exchangePendrin-null miceProximal tubule cellsBrush borderFormate exchangeRenal proximal tubulesSlc26a6-null miceAcid secretionPendrin expressionFunctional expression studiesTubule cellsNaCl reabsorptionCl absorptionBrush border membraneSulfate cotransportLines of evidenceExchanger isoformsTubulesMiceFamily membersBorder membrane
2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro
2000
Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*
Biemesderfer D, DeGray B, Aronson P. Active (9.6 S) and Inactive (21 S) Oligomers of NHE3 in Microdomains of the Renal Brush Border*. Journal Of Biological Chemistry 2000, 276: 10161-10167. PMID: 11120742, DOI: 10.1074/jbc.m008098200.Peer-Reviewed Original ResearchAcridine OrangeAnimalsAntibodiesCarrier ProteinsCell MembraneCentrifugation, Density GradientElectrophoresis, Polyacrylamide GelEndosomesFluorescent Antibody Technique, IndirectGoatsHeymann Nephritis Antigenic ComplexHydrogenHydrogen-Ion ConcentrationImmunoblottingImmunohistochemistryKidneyKidney TubulesMagnesiumMaleMembrane GlycoproteinsMicrofilament ProteinsMicroscopy, ElectronMicrovilliMitochondriaPrecipitin TestsProtein BindingProtein Structure, TertiaryRabbitsRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSucroseTime Factors
1997
Sodium/Hydrogen Exchanger Gene Defect in Slow-Wave Epilepsy Mutant Mice
Cox G, Lutz C, Yang C, Biemesderfer D, Bronson R, Fu A, Aronson P, Noebels J, Frankel W. Sodium/Hydrogen Exchanger Gene Defect in Slow-Wave Epilepsy Mutant Mice. Cell 1997, 91: 139-148. PMID: 9335342, DOI: 10.1016/s0092-8674(01)80016-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtaxiaBrain ChemistryCell LineCerebellumCerebral CortexChromosome MappingCrosses, GeneticElectroencephalographyEpilepsyFibroblastsGenes, RecessiveIon TransportMiceMice, Inbred C57BLMice, Neurologic MutantsOrgan SpecificityPhenotypePoint MutationRNASodiumSodium-Hydrogen ExchangersConceptsSodium/hydrogen exchangerSpontaneous mouse mutantDisease-causing mutationsPlasma membraneChromosome 4Null allelesMouse mutantsCell survivalHydrogen exchangerNHE genesMutantsGene defectsMutant miceNHE1Growth factorTonic-clonic seizuresSelective neuronal deathNeuronal deathDelicate balanceNeurological syndromeEpilepsy phenotypeGenesNew toolCell volumeMutations
1996
Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*
Wu M, Biemesderfer D, Giebisch G, Aronson P. Role of NHE3 in Mediating Renal Brush Border Na+-H+ Exchange ADAPTATION TO METABOLIC ACIDOSIS*. Journal Of Biological Chemistry 1996, 271: 32749-32752. PMID: 8955109, DOI: 10.1074/jbc.271.51.32749.Peer-Reviewed Original ResearchConceptsMetabolic acidosisControl ratsBrush border membraneRenal brush border vesiclesNHE3 proteinBrush border vesiclesBorder membraneBrush borderNHE3 protein expressionRole of NHE3Western blot analysisNormal ratsAcidosisRatsAcidotic ratsRenal brush border membranePharmacologic inhibitorsProtein expressionExchange activityInhibitor sensitivityNHE3Renal brush borderBlot analysisPathways for Oxalate Transport in Rabbit Renal Microvillus Membrane Vesicles*
Kuo S, Aronson P. Pathways for Oxalate Transport in Rabbit Renal Microvillus Membrane Vesicles*. Journal Of Biological Chemistry 1996, 271: 15491-15497. PMID: 8663096, DOI: 10.1074/jbc.271.26.15491.Peer-Reviewed Original Research
1995
1994 Homer W. Smith Award. From flies to physiology--accidental findings along the trail of renal NaCl transport.
Aronson PS. 1994 Homer W. Smith Award. From flies to physiology--accidental findings along the trail of renal NaCl transport. Journal Of The American Society Of Nephrology 1995, 5: 2001-13. PMID: 7579047, DOI: 10.1681/asn.v5122001.Commentaries, Editorials and Letters
1991
Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript
Hildebrandt F, Pizzonia J, Reilly R, Rebouças N, Sardet C, Pouysségur J, Slayman C, Aronson P, Igarashi P. Cloning, sequence, and tissue distribution of a rabbit renal Na+/H+ exchanger transcript. Biochimica Et Biophysica Acta 1991, 1129: 105-108. PMID: 1661611, DOI: 10.1016/0167-4781(91)90221-7.Peer-Reviewed Original ResearchA Ca-activated K channel from rabbit renal brush-border membrane vesicles in planar lipid bilayers
Zweifach A, Desir GV, Aronson PS, Giebisch GH. A Ca-activated K channel from rabbit renal brush-border membrane vesicles in planar lipid bilayers. American Journal Of Physiology 1991, 261: f187-f196. PMID: 1713419, DOI: 10.1152/ajprenal.1991.261.1.f187.Peer-Reviewed Original ResearchCation specificity and modes of the Na+:CO3(2-):HCO3- cotransporter in renal basolateral membrane vesicles
Soleimani M, Lesoine GA, Bergman JA, Aronson PS. Cation specificity and modes of the Na+:CO3(2-):HCO3- cotransporter in renal basolateral membrane vesicles. Journal Of Biological Chemistry 1991, 266: 8706-8710. PMID: 2026588, DOI: 10.1016/s0021-9258(18)31503-5.Peer-Reviewed Original ResearchIncreased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells
Reilly RF, Haggerty JG, Aronson PS, Adelberg EA, Slayman CW. Increased Na(+)-H+ antiporter activity in apical membrane vesicles from mutant LLC-PK1 cells. American Journal Of Physiology 1991, 260: c738-c744. PMID: 1673319, DOI: 10.1152/ajpcell.1991.260.4.c738.Peer-Reviewed Original ResearchHigh affinity binding of amiloride analogs at an internal site in renal microvillus membrane vesicles
Desir GV, Cragoe EJ, Aronson PS. High affinity binding of amiloride analogs at an internal site in renal microvillus membrane vesicles. Journal Of Biological Chemistry 1991, 266: 2267-2271. PMID: 1846621, DOI: 10.1016/s0021-9258(18)52238-9.Peer-Reviewed Original ResearchConceptsMembrane vesiclesMicrovillus membrane vesiclesRenal microvillus membrane vesiclesSimilar rank order potencyBinding sitesInternal sitesHigh-affinity inhibitorsMembrane localizationInternal binding siteAmiloride analoguesPlasma membraneRank order potencyHigh-affinity bindingAffinity inhibitorsHigh affinity-binding siteVesiclesBasolateral membrane vesiclesOrder potency
1990
Characterization of Na(+)-H+ exchangers on villus cells in rabbit ileum
Knickelbein RG, Aronson PS, Dobbins JW. Characterization of Na(+)-H+ exchangers on villus cells in rabbit ileum. American Journal Of Physiology 1990, 259: g802-g806. PMID: 2173416, DOI: 10.1152/ajpgi.1990.259.5.g802.Peer-Reviewed Original Research
1989
Ionic mechanism of Na+-HCO3 − cotransport in rabbit renal basolateral membrane vesicles*
Soleimani M, Aronson PS. Ionic mechanism of Na+-HCO3 − cotransport in rabbit renal basolateral membrane vesicles*. Journal Of Biological Chemistry 1989, 264: 18302-18308. PMID: 2509453, DOI: 10.1016/s0021-9258(18)51463-0.Peer-Reviewed Original Research4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAnimalsAnionsBicarbonatesBinding, CompetitiveBiological TransportCarbon DioxideCarrier ProteinsCell MembraneHydrogen-Ion ConcentrationKidneyLithiumMaleRabbitsSodiumSodium-Bicarbonate SymportersStilbenesSulfitesEffects of acetazolamide on Na+-HCO-3 cotransport in basolateral membrane vesicles isolated from rabbit renal cortex.
Soleimani M, Aronson PS. Effects of acetazolamide on Na+-HCO-3 cotransport in basolateral membrane vesicles isolated from rabbit renal cortex. Journal Of Clinical Investigation 1989, 83: 945-951. PMID: 2921327, PMCID: PMC303770, DOI: 10.1172/jci113980.Peer-Reviewed Original ResearchMeSH Keywords4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAcetatesAcetazolamideAmmoniaAnimalsBicarbonatesBiological TransportCarrier ProteinsCell MembraneHydrogen-Ion ConcentrationKidney CortexMaleRabbitsSodiumSodium-Bicarbonate Symporters