2018
Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells
Lu M, Li JR, Alvarez-Lugo L, Li Y, Yu S, Li X, Shi B, Chai TC. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. American Journal Of Physiology - Cell Physiology 2018, 314: c643-c653. PMID: 29466671, DOI: 10.1152/ajpcell.00339.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic AMP-Dependent Protein KinasesFemaleLarge-Conductance Calcium-Activated Potassium Channel alpha SubunitsLipopolysaccharide ReceptorsLipopolysaccharidesLymphocyte Antigen 96Membrane PotentialsMice, Inbred C57BLPatch-Clamp TechniquesPotassiumSignal TransductionToll-Like Receptor 4Urinary BladderUrinary Tract InfectionsUrotheliumConceptsBK channel activityBK channelsChannel activityUmbrella cellsUrinary tract infectionFemale C57BL6 micePotassium channel inhibitorsUropathogenic Escherichia coliProtein kinase ATract infectionsLPS effectsC57BL6 miceBladder urotheliumLamina propriaMin of exposureChannel inhibitorsBacterial infectionsLPS receptorUrothelial tissuesUrothelial cellsUrotheliumLipopolysaccharidePotassium channelsRT-PCRPKA blocker
2012
SeSAME/EAST syndrome—phenotypic variability and delayed activity of the distal convoluted tubule
Scholl UI, Dave HB, Lu M, Farhi A, Nelson-Williams C, Listman JA, Lifton RP. SeSAME/EAST syndrome—phenotypic variability and delayed activity of the distal convoluted tubule. Pediatric Nephrology 2012, 27: 2081-2090. PMID: 22907601, DOI: 10.1007/s00467-012-2219-4.Peer-Reviewed Original ResearchMeSH KeywordsAbsorptionAge FactorsBiomarkersChildDNA Mutational AnalysisElectrolytesFemaleGenetic Predisposition to DiseaseHearing Loss, SensorineuralHEK293 CellsHomozygoteHumansInfantIntellectual DisabilityKidney Tubules, DistalMaleMembrane PotentialsMicroscopy, ConfocalMutationPedigreePhenotypePotassium Channels, Inwardly RectifyingPredictive Value of TestsSeizuresTransfectionConceptsGitelman syndromeAutosomal recessive syndromeRenal featuresElectrolyte disordersHypokalemic alkalosisClinical presentationElectrolyte imbalanceMedical recordsSignificant worseningBiochemical lossSensorineural deafnessSalt reabsorptionConclusionsThese findingsRecessive syndromeSimilar findingsSyndromeAge 5Mental retardationUnreported familiesSeizuresChannel functionAgeAffected membersFirst yearTubules
2002
Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD)
Lu M, Hebert SC, Giebisch G. Hydrolyzable ATP and PIP2 Modulate the Small-conductance K+ Channel in Apical Membranes of Rat Cortical-Collecting Duct (CCD). The Journal Of General Physiology 2002, 120: 603-615. PMID: 12407074, PMCID: PMC2229550, DOI: 10.1085/jgp.20028677.Peer-Reviewed Original ResearchMeSH Keywords1-Phosphatidylinositol 4-KinaseAdenosine TriphosphateAnimalsCell MembraneCyclic AMP-Dependent Protein KinasesElectrophysiologyHydrolysisKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsPatch-Clamp TechniquesPhosphatidylinositol 4,5-DiphosphatePhosphorylationPotassium ChannelsPotassium Channels, Inwardly RectifyingProtein Interaction MappingRatsRats, Sprague-Dawley
2001
Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD
Wei Y, Lu M, Wang W. Ca2+ mediates the effect of inhibition of Na+-K+-ATPase on the basolateral K+ channels in the rat CCD. American Journal Of Physiology - Cell Physiology 2001, 280: c920-c928. PMID: 11245609, DOI: 10.1152/ajpcell.2001.280.4.c920.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzylaminesBiological TransportCalciumCalcium-Calmodulin-Dependent Protein KinasesDose-Response Relationship, DrugEnzyme InhibitorsFemaleIon Channel GatingIonomycinIonophoresKidney Tubules, CollectingMaleMembrane PotentialsNaphthalenesNG-Nitroarginine Methyl EsterNitratesPotassium ChannelsProtein Kinase CRatsRats, Sprague-DawleySodium-Potassium-Exchanging ATPaseSpecific Pathogen-Free OrganismsStrophanthidinSulfonamidesSuperoxidesConceptsNitro-L-arginine methyl esterIntracellular Ca2Inhibitory effectNM Ca2Nitric oxideChannel activityEffects of strophanthidinKN-93KN-62Protein kinase CCalphostin CEffect of inhibitionCalmodulin-dependent kinase IICell-attached patchesExtracellular Ca2Rat CCDKinase CMicroM ionomycinRat kidneyMechanism of Ca2High concentrationsStrophanthidinCa2Methyl esterKinase II
2000
Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney
Lu M, MacGregor G, Wang W, Giebisch G. Extracellular Atp Inhibits the Small-Conductance K Channel on the Apical Membrane of the Cortical Collecting Duct from Mouse Kidney. The Journal Of General Physiology 2000, 116: 299-310. PMID: 10919872, PMCID: PMC2229488, DOI: 10.1085/jgp.116.2.299.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAffinity LabelsAlkaloidsAnimalsCarbazolesColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesCyclic GMP-Dependent Protein KinasesEnzyme InhibitorsExtracellular SpaceIndolesIon Channel GatingKidney Tubules, CollectingMembrane PotentialsMiceMice, Inbred C57BLNaphthalenesNG-Nitroarginine Methyl EsterOkadaic AcidPatch-Clamp TechniquesPhosphoprotein PhosphatasesPhosphorylationPotassiumPotassium ChannelsPotassium Channels, Calcium-ActivatedProtein KinasesRatsRats, Sprague-DawleyReceptors, PurinergicSmall-Conductance Calcium-Activated Potassium ChannelsThionucleotidesUridine TriphosphateConceptsApical membraneChannel activityProtein kinase AProtein kinase C.Protein phosphatasePurinergic receptor stimulationSmall-conductance potassium channelsOkadaic acidExtracellular ATP concentrationKinase AKinase C.Addition of ATPG proteinsRat homologuePhospholipase CPhosphatase activitySK activitySmall-conductance K channelsApical receptorsATP inhibitsSingle-channel analysisATPNucleotide sensitivityApical K channelsK channelsTwo Types of K+ Channels are Present in the Apical Membrane of the Thick Ascending Limb of the Mouse Kidney
Lu M, Wang W. Two Types of K+ Channels are Present in the Apical Membrane of the Thick Ascending Limb of the Mouse Kidney. Kidney & Blood Pressure Research 2000, 23: 75-82. PMID: 10765108, DOI: 10.1159/000025957.Peer-Reviewed Original ResearchAdenosine TriphosphateAnimalsBariumBiophysical PhenomenaBiophysicsCell MembraneCytosolElectrophysiologyIntermediate-Conductance Calcium-Activated Potassium ChannelsKidneyMembrane PotentialsMicePatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsPotassium Channels, Calcium-ActivatedSmall-Conductance Calcium-Activated Potassium ChannelsTetraethylammonium
1998
Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD
Lu M, Wang W. Reaction of nitric oxide with superoxide inhibits basolateral K+ channels in the rat CCD. American Journal Of Physiology 1998, 275: c309-c316. PMID: 9688863, DOI: 10.1152/ajpcell.1998.275.1.c309.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCyclic GMPDitiocarbIn Vitro TechniquesKidney CortexKidney Tubules, CollectingMembrane PotentialsNitratesNitric OxideNitroprussidePatch-Clamp TechniquesPenicillaminePotassium ChannelsPotassium Channels, Calcium-ActivatedPyrogallolRatsRats, Sprague-DawleySmall-Conductance Calcium-Activated Potassium ChannelsS-Nitroso-N-AcetylpenicillamineSuperoxidesConceptsNitric oxideInhibitory effectChannel activityPatch-clamp techniqueCGMP-dependent pathwayMicroM SNAPRat CCDCGMP productionNO donorRat kidneyControl valuesMM TironIntracellular scavengerExogenous cGMPSuperoxide dismutaseOpen probabilityControl conditionHigh concentrationsDiethyldithiocarbamic acidDonorsKidney
1997
Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL
Wang W, Lu M, Balazy M, Hebert S. Phospholipase A2 is involved in mediating the effect of extracellular Ca2+ on apical K+ channels in rat TAL. American Journal Of Physiology 1997, 273: f421-f429. PMID: 9321915, DOI: 10.1152/ajprenal.1997.273.3.f421.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalciumEstrenesFluorescent DyesFura-2GadoliniumHydroxyeicosatetraenoic AcidsKidney CortexKidney MedullaKineticsLoop of HenleMembrane PotentialsPatch-Clamp TechniquesPhosphodiesterase InhibitorsPhospholipases APhospholipases A2Potassium ChannelsPyrrolidinonesRatsRats, Sprague-DawleyTime FactorsConceptsThick ascending limbMicroM Gd3Phospholipase A2Extracellular Ca2Rat thick ascending limbAddition of thapsigarginEffects of stimulationInflux of Ca2Channel activityPatch-clamp techniquePhospholipase CCell-attached patchesMicroM gadoliniumFura-2Ascending limbFluorescent sodium indicatorIntracellular Ca2Rat kidneyInhibitory effectAcetoxymethyl esterInhibitionStimulationCa2Signaling mechanismSodium indicatorNitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD
Lu M, Giebisch G, Wang W. Nitric oxide-induced hyperpolarization stimulates low-conductance Na+ channel of rat CCD. American Journal Of Physiology 1997, 272: f498-f504. PMID: 9140051, DOI: 10.1152/ajprenal.1997.272.4.f498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCyclic GMPElectric ConductivityKidney CortexKidney Tubules, CollectingKineticsMembrane PotentialsModels, BiologicalNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePatch-Clamp TechniquesPenicillamineRatsRats, Sprague-DawleyS-Nitroso-N-AcetylpenicillamineSodium ChannelsTime FactorsConceptsL-NAMESodium nitroprussideNitric oxideNitro-L-arginine methyl esterL-NAME-induced decreaseAction of NOL-NAME resultsS-nitroso-N-acetyl penicillamineEffect of NOEffect of SNAPWhole-cell recordingsMicroM sodium nitroprussidePatch-clamp techniqueExogenous NO donorD-NAMENO synthaseMicroM amilorideRat CCDCell recordingsNO donorControl valuesStimulatory effectIndirect actionDependent pathwayHyperpolarization