Molecular testing opportunities on cytology effusion specimens: the pre-analytic effects of various body fluid cytology preparation methods on RNA extraction quality and targeted sequencing
Sura G, Tran K, Fu C, Du L, Marczyk M, Martinez Y, Tinnirello A, Gould R, Lau R, Symmans W. Molecular testing opportunities on cytology effusion specimens: the pre-analytic effects of various body fluid cytology preparation methods on RNA extraction quality and targeted sequencing. Journal Of The American Society Of Cytopathology 2022, 12: 10-19. PMID: 36270909, PMCID: PMC10644714, DOI: 10.1016/j.jasc.2022.09.003.Peer-Reviewed Original ResearchConceptsCell blocksCarnoy's solutionDiff-QuikFF samplesCytology laboratoryFFPE cell blocksMultigene expression signaturesConcordance correlation coefficientCell block sectionsParaffin-embedded sectionsMalignant effusionsEffusion specimensMolecular subtypesSurePath samplesCytospin preparationsCytologic methodsCytologic samplesSurePath preparationsDiagnostic approachClinical researchRNA sequencing analysisExpression signaturesHigh concordanceBlock sectionsFrozen cell pellets