2023
Combining Low-Dose Computer-Tomography-Based Radiomics and Serum Metabolomics for Diagnosis of Malignant Nodules in Participants of Lung Cancer Screening Studies
Zyla J, Marczyk M, Prazuch W, Sitkiewicz M, Durawa A, Jelitto M, Dziadziuszko K, Jelonek K, Kurczyk A, Szurowska E, Rzyman W, Widłak P, Polanska J. Combining Low-Dose Computer-Tomography-Based Radiomics and Serum Metabolomics for Diagnosis of Malignant Nodules in Participants of Lung Cancer Screening Studies. Biomolecules 2023, 14: 44. PMID: 38254644, PMCID: PMC10813699, DOI: 10.3390/biom14010044.Peer-Reviewed Original ResearchSerum metabolomicsComputed tomography lung cancer screeningLow-dose computed tomography (LDCT) lung cancer screeningLung cancer screening studyTomography lung cancer screeningLung cancer screeningCancer screening studyLung cancer diagnosisCollected cohortCancer screeningLung cancerSerum metabolomeBenign lesionsPulmonary nodulesMetabolomic evaluationAuxiliary biomarkerLogistic regressionDiagnosisCancer diagnosisLung nodulesMalignant nodulesScreening studyPatientsRadiomicsMolecular features
2022
Tumor immune microenvironment of self-identified African American and non-African American triple negative breast cancer
Marczyk M, Qing T, O’Meara T, Yagahoobi V, Pelekanou V, Bai Y, Reisenbichler E, Cole KS, Li X, Gunasekharan V, Ibrahim E, Fanucci K, Wei W, Rimm DL, Pusztai L, Blenman KRM. Tumor immune microenvironment of self-identified African American and non-African American triple negative breast cancer. Npj Breast Cancer 2022, 8: 88. PMID: 35869114, PMCID: PMC9307813, DOI: 10.1038/s41523-022-00449-3.Peer-Reviewed Original ResearchTumor immune microenvironmentImmune microenvironmentTriple-negative breast cancer tissuesTriple-negative breast cancerAfrican AmericansImmune checkpoint inhibitorsTumor mutation burdenNegative breast cancerBreast cancer tissuesImmune-related pathwaysStromal TILsCheckpoint inhibitorsImmune exclusionClinical outcomesPD-L1Self-identified African AmericansCancer patientsImmune infiltrationBreast cancerMutation burdenCancer tissuesPredictive signatureMRNA expressionTherapeutic agentsPatients
2021
Analysis of the Applicability of microRNAs in Peripheral Blood Leukocytes as Biomarkers of Sensitivity and Exposure to Fractionated Radiotherapy towards Breast Cancer
Marczyk M, Polańska J, Wojcik A, Lundholm L. Analysis of the Applicability of microRNAs in Peripheral Blood Leukocytes as Biomarkers of Sensitivity and Exposure to Fractionated Radiotherapy towards Breast Cancer. International Journal Of Molecular Sciences 2021, 22: 8705. PMID: 34445424, PMCID: PMC8395710, DOI: 10.3390/ijms22168705.Peer-Reviewed Original ResearchConceptsBreast cancer patientsExternal beam radiotherapyCourse of radiotherapyPeripheral blood leukocytesBiomarkers of sensitivityLogistic regression modelsMultinomial logistic regression modelsFractionated RadiotherapyInter-individual variabilityCancer patientsUnivariate analysisBreast cancerCombination of miRNAsBeam radiotherapyBlood leukocytesBlood samplesPromising biomarkerTotal doseSensitive biomarkerRadiotherapyPatientsStrong inter-individual variabilityBiomarkersDoseLeukocytes
2020
Germline variant burden in cancer genes correlates with age at diagnosis and somatic mutation burden
Qing T, Mohsen H, Marczyk M, Ye Y, O’Meara T, Zhao H, Townsend JP, Gerstein M, Hatzis C, Kluger Y, Pusztai L. Germline variant burden in cancer genes correlates with age at diagnosis and somatic mutation burden. Nature Communications 2020, 11: 2438. PMID: 32415133, PMCID: PMC7228928, DOI: 10.1038/s41467-020-16293-7.Peer-Reviewed Original ResearchConceptsAge groupsGermline variantsSomatic mutationsLate-onset cancerEarly-onset cancersCancer hallmark genesSomatic mutation burdenMutation burdenMalignant transformationCancer genesYounger ageGermline alterationsCancerVariant burdenBurdenAverage numberHallmark genesAgeNegative correlationStrong negative correlationMutationsPatientsGroupMolecular Profiling for Predictors of Radiosensitivity in Patients with Breast or Head-and-Neck Cancer
Drobin K, Marczyk M, Halle M, Danielsson D, Papiez A, Sangsuwan T, Bendes A, Hong MG, Qundos U, Harms-Ringdahl M, Wersäll P, Polanska J, Schwenk JM, Haghdoost S. Molecular Profiling for Predictors of Radiosensitivity in Patients with Breast or Head-and-Neck Cancer. Cancers 2020, 12: 753. PMID: 32235817, PMCID: PMC7140105, DOI: 10.3390/cancers12030753.Peer-Reviewed Original ResearchSingle nucleotide polymorphismsCancer patientsRadiosensitive patientsProteomic dataDNA repairSNP variantsRadiation-induced toxicityNeck cancer patientsLong-term survivalPredictors of radiosensitivityNucleotide polymorphismsProteinAdverse health effectsPlasma proteomicsGrowth capacityNeck cancerPredictive proteinsBreast cancerImmune responsePatient radiosensitivityPatientsStudy setDNA genotypingMolecular profilingNormal tissues
2016
Serum mass profile signature as a biomarker of early lung cancer
Widlak P, Pietrowska M, Polanska J, Marczyk M, Ros-Mazurczyk M, Dziadziuszko R, Jassem J, Rzyman W. Serum mass profile signature as a biomarker of early lung cancer. Lung Cancer 2016, 99: 46-52. PMID: 27565913, DOI: 10.1016/j.lungcan.2016.06.011.Peer-Reviewed Original ResearchConceptsEarly lung cancerLung cancerNegative predictive valuePredictive valueComputed tomographyEarly-stage lung cancerLow-dose computed tomographyIndeterminate pulmonary nodulesStage lung cancerPotential clinical usefulnessPositive predictive valueValidation cohortHealthy controlsClinical usefulnessDiscovery cohortBlood samplesIndependent cohortWeight serum fractionsPulmonary nodulesIndependent sample setCancerPatientsHealthy participantsMolecular biomarkersCohort
2015
Influence of genetic background and oxidative stress response on risk of mandibular osteoradionecrosis after radiotherapy of head and neck cancer
Danielsson D, Brehwens K, Halle M, Marczyk M, Sollazzo A, Polanska J, Munck-Wikland E, Wojcik A, Haghdoost S. Influence of genetic background and oxidative stress response on risk of mandibular osteoradionecrosis after radiotherapy of head and neck cancer. Head & Neck 2015, 38: 387-393. PMID: 25352150, DOI: 10.1002/hed.23903.Peer-Reviewed Original ResearchConceptsRadiotherapy of headGlutathione S-transferase P1Mandibular osteoradionecrosisNeck cancerCohort of patientsIndividual radiosensitivitySingle nucleotide polymorphismsRadiation-induced oxidative stressDihydro-2'-deoxyguanosine levelsNeck radiotherapy treatmentConcomitant brachytherapyOxidative stress responseSevere complicationsPatient cohortOsteoradionecrosisSNP rs1695Multivariate analysisTissue damageHealthy tissue damagePatientsOxidative stressRadiotherapy treatmentRadiotherapyCohortBiomarker analysis
2014
Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients
Jelonek K, Pietrowska M, Ros M, Zagdanski A, Suchwalko A, Polanska J, Marczyk M, Rutkowski T, Skladowski K, Clench MR, Widlak P. Radiation-Induced Changes in Serum Lipidome of Head and Neck Cancer Patients. International Journal Of Molecular Sciences 2014, 15: 6609-6624. PMID: 24747595, PMCID: PMC4013650, DOI: 10.3390/ijms15046609.Peer-Reviewed Original ResearchConceptsSerum lipidomeConformal radiotherapyMedium dosesEnd of radiotherapyNeck cancer patientsRadiation-induced changesWhole patient bodyBody responsePost-treatment samplesDoses of radiationBlood levelsCancer patientsNeck cancerBlood samplesRadiotherapyNormal tissuesPatientsDosesCancer radiotherapyLipidomePatient's bodyTreatment samplesTissueLipid extractsCancer
2011
Radiation-related Changes in Serum Proteome Profiles Detected by Mass Spectrometry in Blood of Patients Treated with Radiotherapy Due to Larynx Cancer
WIDŁAK P, PIETROWSKA M, WOJTKIEWICZ K, RUTKOWSKI T, WYGODA A, MARCZAK Ł, MARCZYK M, POLAŃSKA J, WALASZCZYK A, DOMIŃCZYK I, SKŁADOWSKI K, STOBIECKI M, POLAŃSKI A. Radiation-related Changes in Serum Proteome Profiles Detected by Mass Spectrometry in Blood of Patients Treated with Radiotherapy Due to Larynx Cancer. Journal Of Radiation Research 2011, 52: 575-581. PMID: 21768750, DOI: 10.1269/jrr.11019.Peer-Reviewed Original ResearchConceptsMass spectrometrySerum samplesLarynx cancerTotal dosesSerum proteomeEnd of radiotherapyStart of radiotherapyRadiation-related changesBlood of patientsConsecutive blood samplesSerum proteome profilesMALDI-TOF mass spectrometryEarly time pointsHuman serum proteomeRadical radiotherapyPeptide ionsBlood samplesPatientsProteome profilesFinal irradiationRetrospective markerPilot studyRadiotherapyTime pointsAnalysed groupAssociation between plasma proteome profiles analysed by mass spectrometry, a lymphocyte-based DNA-break repair assay and radiotherapy-induced acute mucosal reaction in head and neck cancer patients
Pietrowska M, Polańska J, Walaszczyk A, Wygoda A, Rutkowski T, Składowski K, Marczak Ł, Stobiecki M, Marczyk M, Polański A, Widłak P. Association between plasma proteome profiles analysed by mass spectrometry, a lymphocyte-based DNA-break repair assay and radiotherapy-induced acute mucosal reaction in head and neck cancer patients. International Journal Of Radiation Biology 2011, 87: 711-719. PMID: 21351848, DOI: 10.3109/09553002.2011.556174.Peer-Reviewed Original ResearchConceptsAcute mucosal reactionsAcute reactionsMucosal reactionsNeck cancerPlasma proteomeNeck cancer patientsPlasma proteome profilesDefinitive radiotherapyCancer patientsPredictive markerHealthy donorsBlood samplesHealthy individualsPatientsRadiotherapyPlasma samplesComet assayLymphocytesCancerMaximal intensityMarkersProteome profiles