2016
Artificial bacterial biomimetic nanoparticles synergize pathogen-associated molecular patterns for vaccine efficacy
Siefert AL, Caplan MJ, Fahmy TM. Artificial bacterial biomimetic nanoparticles synergize pathogen-associated molecular patterns for vaccine efficacy. Biomaterials 2016, 97: 85-96. PMID: 27162077, PMCID: PMC5999034, DOI: 10.1016/j.biomaterials.2016.03.039.Peer-Reviewed Original ResearchConceptsMonophosphoryl lipid APathogen-associated molecular patternsT cell responsesToll-like receptorsAntigen-specific T-helper 1Antigen-specific T cell responsesCell responsesMolecular patternsAntibody-mediated responsesT helper 1Model antigen ovalbuminBacterial pathogen-associated molecular patternsCytokine profileAntigen-loaded nanoparticlesTLR ligandsCellular immunityHelper 1Vaccine efficacyAntigen ovalbuminVaccine platformImmune responseNanoparticulate vaccinesLipid AOvalbuminCpG
2010
Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro
Pochard P, Vickery B, Berin MC, Grishin A, Sampson HA, Caplan M, Bottomly K. Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro. Journal Of Allergy And Clinical Immunology 2010, 126: 92-97.e5. PMID: 20538332, PMCID: PMC2902661, DOI: 10.1016/j.jaci.2010.04.003.Peer-Reviewed Original ResearchConceptsToll-like receptorsCrude peanut extractDendritic cellsT cellsBacterial adjuvantDC maturationCytokine productionBone marrow-derived dendritic cellsMarrow-derived dendritic cellsDelivery of allergensFood allergy treatmentOngoing allergic responseSuccessful immunotherapeutic strategiesIFN-gamma levelsT cell responsesPrimed T cellsT cell proliferationInnate immune systemCarboxyfluorescein succinimidyl esterAllergen immunotherapySensitized miceBioPlex assayIL-17Immunotherapeutic strategiesTh2 responses
2009
Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy
Demento SL, Eisenbarth SC, Foellmer HG, Platt C, Caplan MJ, Saltzman W, Mellman I, Ledizet M, Fikrig E, Flavell RA, Fahmy TM. Inflammasome-activating nanoparticles as modular systems for optimizing vaccine efficacy. Vaccine 2009, 27: 3013-3021. PMID: 19428913, PMCID: PMC2695996, DOI: 10.1016/j.vaccine.2009.03.034.Peer-Reviewed Original ResearchMeSH KeywordsAdjuvants, ImmunologicAnimalsAntibody FormationCarrier ProteinsCD8-Positive T-LymphocytesDendritic CellsLactic AcidLipopolysaccharidesMiceMice, Inbred C57BLNanoparticlesNLR Family, Pyrin Domain-Containing 3 ProteinPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerVaccinationViral Envelope ProteinsWest Nile FeverWest Nile Virus VaccinesConceptsPattern recognition receptorsToll-like receptorsInflammasome activationInnate immune system activationEffective adaptive immune responseIntracellular pattern recognition receptorsAntigen-presenting cellsAdaptive immune responsesWest Nile encephalitisImmune system activationInnate immune pathwaysWild-type macrophagesDendritic cellsCellular immunityVaccination approachesVaccine efficacyIL-1betaNLRP3 inflammasomeAdjuvant systemImmune responsePotent new approachMurine modelInflammasome activitySystem activationImmune pathwaysInflammasome-activating biodegradable nanoparticulates as vaccine delivery systems (135.80)
Fahmy T, DEMENTO S, Eisenbarth S, Caplan M, Saltzman W, Mellman I, Ledizet M, Fikrig E, Flavell R. Inflammasome-activating biodegradable nanoparticulates as vaccine delivery systems (135.80). The Journal Of Immunology 2009, 182: 135.80-135.80. DOI: 10.4049/jimmunol.182.supp.135.80.Peer-Reviewed Original ResearchToll-like receptorsIL-1βInflammasome activationProinflammatory cytokines IL-1βAntigen-presenting cellsClass of nanomaterialsCytokines IL-1βSurface of nanoparticlesWest Nile encephalitisInnate immune pathwaysVaccine delivery systemWild-type macrophagesCellular immunityVaccination approachesMurine modelInflammasome activityType macrophagesImmune pathwaysAdjuvant deliveryDanger signalsVaccine designModel antigenLipopolysaccharideAntigenBiocompatible polyester