2019
Development of a live attenuated trivalent porcine rotavirus A vaccine against disease caused by recent strains most prevalent in South Korea
Park J, Alfajaro M, Cho E, Kim J, Soliman M, Baek Y, Park C, Lee J, Son K, Cho K, Kang M. Development of a live attenuated trivalent porcine rotavirus A vaccine against disease caused by recent strains most prevalent in South Korea. Veterinary Research 2019, 50: 2. PMID: 30616694, PMCID: PMC6323864, DOI: 10.1186/s13567-018-0619-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsRepublic of KoreaRotavirusRotavirus InfectionsSwineSwine DiseasesVaccines, AttenuatedViral VaccinesConceptsVaccine strainTrivalent vaccineKorean swine industrySevere economic lossesHomologous virulent strainPorcine rotavirusSwine industryEconomic lossesSerum virus-neutralizing antibodiesExperimental pigletsVirulent strainCell culture passageFecal secretory IgAChallenge exposureVirus-neutralizing antibodiesRecent strainsPigletsVirulentConsecutive passagesRotavirus diseaseIgA levelsRotavirus vaccineOral immunizationHistopathological lesionsVaccine
2018
Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II
Soliman M, Kim D, Kim C, Seo J, Kim J, Park J, Alfajaro M, Baek Y, Cho E, Park S, Kang M, Chang K, Goodfellow I, Cho K. Porcine sapovirus Cowden strain enters LLC-PK cells via clathrin- and cholesterol-dependent endocytosis with the requirement of dynamin II. Veterinary Research 2018, 49: 92. PMID: 30223898, PMCID: PMC6142377, DOI: 10.1186/s13567-018-0584-0.Peer-Reviewed Original ResearchConceptsDynamin IIActin rearrangementLate endosomesDN mutantsLLC-PK cellsDynamin GTPase activityClathrin-mediated endocytosisCowden strainClathrin-mediated internalizationDominant negative mutantCholesterol-dependent endocytosisInhibition of caveolaeVesicle internalizationCholesterol-sequestering drugEndosomal traffickingEarly endosomesSiRNA depletionNegative mutantGTPase activityClathrinEndocytosisEndosomal acidificationMutantsEndosomesCell entry
2016
Occurrence and molecular characterization of Sapelovirus A in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm
BAK G, KANG M, SON K, PARK J, KIM D, SEO J, KIM J, ALFAJARO M, SOLIMAN M, BAEK Y, CHO E, Joseph K, CHOI J, PARK S, CHO K. Occurrence and molecular characterization of Sapelovirus A in diarrhea and non-diarrhea feces of different age group pigs in one Korean pig farm. Journal Of Veterinary Medical Science 2016, 78: 16-0237. PMID: 27616554, PMCID: PMC5240775, DOI: 10.1292/jvms.16-0237.Peer-Reviewed Original ResearchPathogenesis of Korean SapelovirusA in piglets and chicks
Kim D, Kang M, Son K, Bak G, Park J, Hosmillo M, Seo J, Kim J, Alfajaro M, Soliman M, Baek Y, Cho E, Lee J, Kwon J, Choi J, Goodfellow I, Cho K. Pathogenesis of Korean SapelovirusA in piglets and chicks. Journal Of General Virology 2016, 97: 2566-2574. PMID: 27487773, PMCID: PMC5078829, DOI: 10.1099/jgv.0.000571.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChickensIntestinesPicornaviridaePicornaviridae InfectionsPoultry DiseasesSwineSwine DiseasesVirulenceConceptsExtra-intestinal organsHigher viral RNA levelsNon-suppurative myelitisViral RNA levelsReal-time reverse transcription PCRCells of pigsIntestinal pathologyReverse transcription-PCRViral antigensViral pathogenesisReproductive disordersSapelovirus APathogenesisPorcine sapelovirusVirus entryCultured cell linesRNA levelsEpithelial cellsTranscription-PCRFecal samplesPigletsPneumoniaCell linesSialic acidPathologyWhole genomic characterization of Korean porcine G8P[7] reassortant rotaviruses
Park J, Park S, Woo N, Kim D, Seo J, Alfajaro M, Kim J, Soliman M, Baek Y, Cho E, Kwon J, Choi J, Kang M, Matthijnssens J, Cho K. Whole genomic characterization of Korean porcine G8P[7] reassortant rotaviruses. Archives Of Virology 2016, 161: 2835-2841. PMID: 27393603, DOI: 10.1007/s00705-016-2945-1.Peer-Reviewed Original Research
2015
Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses
Jeong Y, Matthijnssens J, Kim D, Kim J, Alfajaro M, Park J, Hosmillo M, Son K, Soliman M, Baek Y, Kwon J, Choi J, Kang M, Cho K. Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses. Veterinary Microbiology 2015, 176: 61-69. PMID: 25592760, DOI: 10.1016/j.vetmic.2014.12.024.Peer-Reviewed Original ResearchConceptsGenetic diversityPorcine group C rotavirusPorcine RVCRVC strainsLarge genetic diversityVP6 geneGroup C rotavirusFrequent reassortment eventsStrong geographical differencesPhylogenetic dataPhylogenetic analysisHost speciesHuman RVCORF sequencesC rotavirusReassortment eventsGene segmentsPig industryG7 genotypeImportant pathogenGenesDiversityEconomic impactVP4 sequencesDefinitive cut
2014
Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus
Kim D, Hosmillo M, Alfajaro M, Kim J, Park J, Son K, Ryu E, Sorgeloos F, Kwon H, Park S, Lee W, Cho D, Kwon J, Choi J, Kang M, Goodfellow I, Cho K. Both α2,3- and α2,6-Linked Sialic Acids on O-Linked Glycoproteins Act as Functional Receptors for Porcine Sapovirus. PLOS Pathogens 2014, 10: e1004172. PMID: 24901849, PMCID: PMC4047124, DOI: 10.1371/journal.ppat.1004172.Peer-Reviewed Original ResearchConceptsHisto-blood group antigensFunctional receptorsSambucus nigra lectinSialic acidGroup antigensTreatment of cellsSynthetic histo-blood group antigensViral attachmentVirus bindingMaackia amurensis lectinPorcine sapovirusVibrio cholerae neuraminidaseRed blood cellsIntestinal tissue sectionsAcute gastroenteritisImportant causeInfectionBlood cellsVirus attachmentReceptorsPSAVSapovirusCellular receptorsTissue sectionsGlycoprotein actsComparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves
Park J, Kim D, Matthijnssens J, Kwon H, Zeller M, Alfajaro M, Son K, Hosmillo M, Ryu E, Kim J, Lee J, Park S, Kang M, Kwon J, Choi J, Cho K. Comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. Veterinary Microbiology 2014, 172: 51-62. PMID: 24861840, DOI: 10.1016/j.vetmic.2014.04.010.Peer-Reviewed Original ResearchConceptsHost range restrictionGenotype constellationHistopathological changesCritical molecular determinantsSmall intestineReassortant rotavirusesComparison of pathogenicityDiarrheic pigletsPathological phenotypesDifferent virulenceReassortant strainsPigletsCalvesNSP4 genesSpecific mutationsAmino acid substitutionsRotavirus evolutionMolecular determinantsNSP3 genePathogenicityVirulenceAcid substitutionsNucleotide changesDiarrheaRotavirusAnti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract
Alfajaro M, Rho M, Kim H, Park J, Kim D, Hosmillo M, Son K, Lee J, Park S, Kang M, Ryu Y, Park K, Oh H, Lee S, Park S, Lee W, Cho K. Anti-rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Research In Veterinary Science 2014, 96: 567-575. PMID: 24704033, DOI: 10.1016/j.rvsc.2014.03.011.Peer-Reviewed Original ResearchConceptsCombination therapyAnti-rotaviral activityAnti-rotavirus effectIntestinal lesion scoresLethal dose 50Rotavirus enteritisCurative medicationsRotaviral enteritisSophora flavescensRotaviral diarrheaOrgan weightsPiglet modelPathological changesFecal virusBody weightTherapyVirus replicationLesion scoresSingle treatmentDose 50Porcine rotavirusAdverse effectsPreliminary dataDiarrheaEnteritis
2013
Molecular epidemiology of Korean porcine sapeloviruses
Son K, Kim D, Matthijnssens J, Kwon H, Park J, Hosmillo M, Alfajaro M, Ryu E, Kim J, Kang M, Cho K. Molecular epidemiology of Korean porcine sapeloviruses. Archives Of Virology 2013, 159: 1175-1180. PMID: 24232913, PMCID: PMC7087272, DOI: 10.1007/s00705-013-1901-6.Peer-Reviewed Original ResearchConceptsGenetic diversityPorcine sapelovirusPronounced genetic diversityVP1 geneDiarrhea fecal samplesPhylogenetic analysisPrimer pairsPSV infectionGenesPCR assaysDiversityRT-PCRKorean pigsSapelovirusFecal samplesMolecular epidemiologyDiarrhea samplesBacteriaInfectionEnteric virusesHigher proportionAssaysPigsStrainsDifferent virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves
Park J, Kim H, Matthijnssens J, Alfajaro M, Kim D, Son K, Kwon H, Hosmillo M, Ryu E, Kim J, Cena R, Lee J, Kang M, Park S, Cho K. Different virulence of porcine and porcine-like bovine rotavirus strains with genetically nearly identical genomes in piglets and calves. Veterinary Research 2013, 44: 88. PMID: 24083947, PMCID: PMC3851489, DOI: 10.1186/1297-9716-44-88.Peer-Reviewed Original ResearchConceptsHistopathological changesSmall intestineGroup A rotavirusesDirect interspecies transmissionGenotype constellationCritical molecular determinantsFull-length sequencing analysisA rotavirusesBovine rotavirusVP1-VP4Pathological phenotypesDifferent virulencePigletsDiarrheaInterspecies transmissionAmino acid mutationsRotavirusCalvesIntestineSpecific mutationsTropismMolecular determinantsSequencing analysisUntranslated regionPorcine
2012
Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea
Alfajaro M, Kim H, Park J, Ryu E, Kim J, Jeong Y, Kim D, Hosmillo M, Son K, Lee J, Kwon H, Ryu Y, Park S, Park S, Lee W, Cho K. Anti-rotaviral effects of Glycyrrhiza uralensis extract in piglets with rotavirus diarrhea. Virology Journal 2012, 9: 310. PMID: 23244491, PMCID: PMC3547719, DOI: 10.1186/1743-422x-9-310.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornAnti-Inflammatory Agents, Non-SteroidalAntiviral AgentsCell LineColostrumDiarrheaDose-Response Relationship, DrugDrug Evaluation, PreclinicalFecesGlycyrrhiza uralensisInterleukin-8Intestine, SmallMAP Kinase Signaling SystemModels, AnimalNF-kappa BPhytotherapyPlant ExtractsPlant RootsRNA, MessengerRotavirusRotavirus InfectionsSpleenSwineSwine DiseasesTumor Necrosis Factor-alphaVirus SheddingConceptsInflammation-related cytokinesGlycyrrhiza uralensis extractMRNA expression levelsSmall intestineRotavirus diarrheaRotaviral enteritisAnti-rotaviral effectAnti-inflammatory effectsFecal virus sheddingOnset of diarrheaColostrum-deprived pigletsExpression levelsIntestinal lesion scoresFecal consistency scoreAntiviral efficacyVirus sheddingHistological changesSevere gastroenteritisFecal virusAntiviral agentsDiarrheaLesion scoresViable medicationsHerbal medicineAntiviral propertiesPathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets
Kim H, Park J, Alfajaro M, Kim D, Hosmillo M, Son K, Lee J, Bae Y, Park S, Kang M, Cho K. Pathogenicity characterization of a bovine triple reassortant rotavirus in calves and piglets. Veterinary Microbiology 2012, 159: 11-22. PMID: 22465801, DOI: 10.1016/j.vetmic.2012.03.017.Peer-Reviewed Original ResearchConceptsExtra-intestinal organsMesenteric lymph nodesCross-species pathogenicityReassortant rotavirusesLymph nodesIntestinal pathologyPersistent severe diarrheaSevere intestinal pathologyExtra-intestinal infectionsDiarrhea fecal samplesReal-time RT-PCRGreen real-time RT-PCRSYBR Green real-time RT-PCRSevere diarrheaCerebrospinal fluidChoroid plexusViral replicationRotavirusRT-PCRViral RNAFecal samplesPigletsDiarrheaPublic healthPathology
2011
Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets
Kim H, Park J, Matthijnssens J, Lee J, Bae Y, Alfajaro M, Park S, Kang M, Cho K. Intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. Veterinary Microbiology 2011, 152: 291-303. PMID: 21658866, DOI: 10.1016/j.vetmic.2011.05.017.Peer-Reviewed Original ResearchConceptsGroup A rotavirusesExtra-intestinal spreadCross-species pathogenicityPost-inoculation day 1Intestinal villous atrophyVirus-inoculated calvesExtra-intestinal organsMesenteric lymph nodesBovine group A rotavirusesColostrum-deprived calvesViral RNAReal-time RT-PCRNSP4 gene segmentsGreen real-time RT-PCRSYBR Green real-time RT-PCRVillous atrophyLymph nodesClinical symptomsGene segmentsSevere diarrheaCerebrospinal fluidChoroid plexusDay 1Viral replicationReassortant rotaviruses
2010
Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows
Hosmillo M, Jeong Y, Kim H, Park J, Nayak M, Alfajaro M, Collantes T, Park S, Ikuta K, Yunoki M, Kang M, Park S, Cho K. Molecular detection of genotype 3 porcine hepatitis E virus in aborted fetuses and their sows. Archives Of Virology 2010, 155: 1157-1161. PMID: 20458510, DOI: 10.1007/s00705-010-0679-z.Peer-Reviewed Original ResearchConceptsPorcine circovirus type 2Polymerase chain reactionHepatitis E virus RNAGenotype 3 HEVHEV-positive samplesHepatitis E virusReal-time RT-PCRViral copies/HEV infectionHEV RNATransplacental infectionCircovirus type 2E virusCopies/Type 2PCV2 coinfectionSerum samplesFetusesRT-PCRVirus RNAChain reactionReproductive failureInfectionReverse transcriptionMolecular detection