2021
Differential adhesion regulates neurite placement via a retrograde zippering mechanism
Sengupta T, Koonce NL, Vázquez-Martínez N, Moyle MW, Duncan LH, Emerson SE, Han X, Shao L, Wu Y, Santella A, Fan L, Bao Z, Mohler W, Shroff H, Colón-Ramos DA. Differential adhesion regulates neurite placement via a retrograde zippering mechanism. ELife 2021, 10: e71171. PMID: 34783657, PMCID: PMC8843091, DOI: 10.7554/elife.71171.Peer-Reviewed Original ResearchConceptsDifferential adhesionDifferential adhesion mechanismsSYG-1SYG-2Developmental programEmbryonic developmentNeurite tipsZippering mechanismBiophysical principlesNeurite shaftSynaptic specificityBrain neuropilSingle neuriteLayers occursAlternate mechanismAdhesion mechanismExpressionNeuritesZipperingAdhesionMechanismOutgrowth
2019
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution.
Duncan LH, Moyle MW, Shao L, Sengupta T, Ikegami R, Kumar A, Guo M, Christensen R, Santella A, Bao Z, Shroff H, Mohler W, Colón-Ramos DA. Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution. Journal Of Visualized Experiments 2019 PMID: 31233035, PMCID: PMC7255390, DOI: 10.3791/59533.Peer-Reviewed Original ResearchConceptsGene expressionC. elegans embryosCell lineage identityCell lineage analysisSingle-cell resolutionElegans embryosCaenorhabditis elegansC. elegansSelective plane illumination microscopyLight-sheet microscopyLineage analysisNematode embryosCell resolutionEntire nervous systemSubcellular levelOnly organismSubcellular resolutionElegansCellular originSingle cellsEmbryosIllumination microscopyEmbryogenesisMorphological dynamicsExpression