2016
Imaging Functional Nucleic Acid Delivery to Skin
Kaspar RL, Hickerson RP, González-González E, Flores MA, Speaker TP, Rogers FA, Milstone LM, Contag CH. Imaging Functional Nucleic Acid Delivery to Skin. Methods In Molecular Biology 2016, 1372: 1-24. PMID: 26530911, DOI: 10.1007/978-1-4939-3148-4_1.Peer-Reviewed Original ResearchConceptsNucleic acid deliveryNucleic acid-based therapiesAcid deliveryAcid-based therapiesFunctional deliveryFunctional nucleic acid deliveryTherapeutic nucleic acidsNucleic acidsOptical imaging technologiesReal-time monitoringTriplex nucleic acidsInteraction of lightMonogenic skin diseasesLight-tissue interactionsGene editingGene silencingOptical imagingNoninvasive imaging technologyTopical deliveryTime monitoringImaging technologyReporter geneLayered structureGeneral strategyEnzymatic reactions
2012
Local Delivery of Gene-Modifying Triplex-Forming Molecules to the Epidermis
Rogers FA, Hu RH, Milstone LM. Local Delivery of Gene-Modifying Triplex-Forming Molecules to the Epidermis. Journal Of Investigative Dermatology 2012, 133: 685-691. PMID: 23014335, PMCID: PMC3532560, DOI: 10.1038/jid.2012.351.Peer-Reviewed Original ResearchConceptsIntradermal deliveryHairless miceTransgenic miceBasal keratinocytesMethod of deliveryNuclei of keratinocytesIntraperitoneal administrationSitu gene correctionBack skinLocal deliveryMiceDisease-causing genesEpidermal keratinocytesKeratinocytesLocal administrationAdministrationDeliverySkinEpidermisDaysImpact of epidermal desquamation on tissue stores of iron
Milstone LM, Hu RH, Dziura JD, Zhou J. Impact of epidermal desquamation on tissue stores of iron. Journal Of Dermatological Science 2012, 67: 9-14. PMID: 22575277, PMCID: PMC3374011, DOI: 10.1016/j.jdermsci.2012.04.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimicrobial Cationic PeptidesCell ProliferationDisease Models, AnimalEpidermisFemaleGene Expression RegulationHemochromatosisHemochromatosis ProteinHepcidinsHistocompatibility Antigens Class IHomeostasisHuman papillomavirus 16HumansIronKidneyLiverMaleMembrane ProteinsMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMice, TransgenicPapillomavirus InfectionsReceptors, TransferrinConceptsIron metabolismDifferent mouse modelsSignificant reductionHPV16 E7 geneSystemic iron metabolismSystemic proteinsIron statusMouse modelTissue storesE7 genesSystemic levelsEpidermal turnoverInternal organsEpidermal desquamationTransferrin receptorMetabolic studiesKnockout modelsCorneocyte desquamationDesquamationKidneyHuman skinEpidermal homeostasisMetabolismEpidermisSkin
2006
Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis
Milstone LM, Adams BD, Zhou J, Sanchez V, Shofner J. Stratum-Specific Expression of Human Transferrin Receptor Increases Iron in Mouse Epidermis. Journal Of Investigative Dermatology 2006, 126: 648-652. PMID: 16424878, PMCID: PMC2243218, DOI: 10.1038/sj.jid.5700127.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEpidermisHumansIronMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicReceptors, TransferrinConceptsTransgenic miceStratum-specific expressionTransferrin receptorKeratin 14 promoterTransferrin receptor expressionBody iron lossesReceptor expressionHistological changesSite-dependent differencesLittermate controlsNormal epidermisReceptorsMiceMouse epidermisEpidermal desquamationFerritin expressionHuman transferrin receptorInvolucrin promoterKeratinocytesTransgenic animalsEpidermisAgeExtra ironExpressionAnimals
1994
Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development.
Wysolmerski JJ, Broadus AE, Zhou J, Fuchs E, Milstone LM, Philbrick WM. Overexpression of parathyroid hormone-related protein in the skin of transgenic mice interferes with hair follicle development. Proceedings Of The National Academy Of Sciences Of The United States Of America 1994, 91: 1133-1137. PMID: 7508121, PMCID: PMC521468, DOI: 10.1073/pnas.91.3.1133.Peer-Reviewed Original ResearchConceptsFollicle developmentTransgenic miceHair follicle developmentHuman keratin 14 promoterParathyroid hormone-related proteinNormal hair follicle developmentOverexpression of PTHrPHormone-related proteinHormone-related peptideKeratin 14 promoterHumoral hypercalcemiaPTHrP geneNormal tissuesMicePTHrPSkinOverexpressionCellular differentiationEarly stagesHypercalcemiaMalignancySyndromePeptidesFollicles