1992
Methotrexate induces differentiation of human keratinocytes.
Schwartz P, Barnett S, Atillasoy E, Milstone L. Methotrexate induces differentiation of human keratinocytes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 594-598. PMID: 1731329, PMCID: PMC48285, DOI: 10.1073/pnas.89.2.594.Peer-Reviewed Original ResearchConceptsEffect of methotrexateAbility of methotrexateProliferation of keratinocytesProportion of cellsMarkers of differentiationHuman epidermal keratinocytesMethotrexateTherapeutic actionEpidermal keratinocytesMarked increaseHuman keratinocytesKeratinocytesEnvelope proteinSDS/PAGE profilesInvolucrin synthesisNormal cellsTerminal differentiationTissue homeostasisThymine deoxyribonucleotidesDifferentiationCellsPsoriasisCell morphologyReproducible changesDose
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo
1973
Differentiation of lens fibers in explanted embryonic chick lens epithelia
Piatigorsky J, Rothschild S, Milstone L. Differentiation of lens fibers in explanted embryonic chick lens epithelia. Developmental Biology 1973, 34: 334-345. PMID: 4596973, DOI: 10.1016/0012-1606(73)90362-x.Peer-Reviewed Original ResearchConceptsChick lens epitheliumLens epitheliumLens fibersLens epithelial cellsRibosomal clustersRibosomal RNADelta-crystallinTissue cultureSingle-strand breaksFiber differentiationCell membraneLong cellsMean cell lengthCell lengthTotal RNACellular outgrowthDifferentiationEpithelial cellsSurface membraneRNAOrganelle densityChick embryosGranular matrixBiochemical changesCells