2023
Transcriptional signatures of heroin intake and relapse throughout the brain reward circuitry in male mice
Browne C, Futamura R, Minier-Toribio A, Hicks E, Ramakrishnan A, Martínez-Rivera F, Estill M, Godino A, Parise E, Torres-Berrío A, Cunningham A, Hamilton P, Walker D, Huckins L, Hurd Y, Shen L, Nestler E. Transcriptional signatures of heroin intake and relapse throughout the brain reward circuitry in male mice. Science Advances 2023, 9: eadg8558. PMID: 37294757, PMCID: PMC10256172, DOI: 10.1126/sciadv.adg8558.Peer-Reviewed Original ResearchConceptsOpioid use disorderHeroin intakeContext-induced drug-seekingBrain reward circuitryHeroin self-administrationRNA-seqDrug seekingReward circuitryGenome-wide association study dataSelf-administrationHeroin exposureDrug-takingIntegration of RNA-seq dataUse disorderPatterns of transcriptional regulationRNA-seq dataBehavioral outcomesMale miceMolecular changesTranscriptional regulationRegion-specificGene candidatesRNA sequencingHeroinBioinformatics analysisLeveraging base-pair mammalian constraint to understand genetic variation and human disease
Sullivan P, Meadows J, Gazal S, Phan B, Li X, Genereux D, Dong M, Bianchi M, Andrews G, Sakthikumar S, Nordin J, Roy A, Christmas M, Marinescu V, Wang C, Wallerman O, Xue J, Yao S, Sun Q, Szatkiewicz J, Wen J, Huckins L, Lawler A, Keough K, Zheng Z, Zeng J, Wray N, Li Y, Johnson J, Chen J, Paten B, Reilly S, Hughes G, Weng Z, Pollard K, Pfenning A, Forsberg-Nilsson K, Karlsson E, Lindblad-Toh K, Andrews G, Armstrong J, Bianchi M, Birren B, Bredemeyer K, Breit A, Christmas M, Clawson H, Damas J, Di Palma F, Diekhans M, Dong M, Eizirik E, Fan K, Fanter C, Foley N, Forsberg-Nilsson K, Garcia C, Gatesy J, Gazal S, Genereux D, Goodman L, Grimshaw J, Halsey M, Harris A, Hickey G, Hiller M, Hindle A, Hubley R, Hughes G, Johnson J, Juan D, Kaplow I, Karlsson E, Keough K, Kirilenko B, Koepfli K, Korstian J, Kowalczyk A, Kozyrev S, Lawler A, Lawless C, Lehmann T, Levesque D, Lewin H, Li X, Lind A, Lindblad-Toh K, Mackay-Smith A, Marinescu V, Marques-Bonet T, Mason V, Meadows J, Meyer W, Moore J, Moreira L, Moreno-Santillan D, Morrill K, Muntané G, Murphy W, Navarro A, Nweeia M, Ortmann S, Osmanski A, Paten B, Paulat N, Pfenning A, Phan B, Pollard K, Pratt H, Ray D, Reilly S, Rosen J, Ruf I, Ryan L, Ryder O, Sabeti P, Schäffer D, Serres A, Shapiro B, Smit A, Springer M, Srinivasan C, Steiner C, Storer J, Sullivan K, Sullivan P, Sundström E, Supple M, Swofford R, Talbot J, Teeling E, Turner-Maier J, Valenzuela A, Wagner F, Wallerman O, Wang C, Wang J, Weng Z, Wilder A, Wirthlin M, Xue J, Zhang X. Leveraging base-pair mammalian constraint to understand genetic variation and human disease. Science 2023, 380: eabn2937. PMID: 37104612, PMCID: PMC10259825, DOI: 10.1126/science.abn2937.Peer-Reviewed Original ResearchConceptsHuman genomeHuman diseasesCopy-number variationsHeritable human diseasesGenome annotationVariant annotationGenomic positionsGenomic regionsDisease heritabilityFunctional annotationEvolutionary constraintsAssociation studiesCopy-numberGenetic variationGenetic findingsGenomeCell typesRegulatory landscapeDisease mechanismsAnnotationBiological mechanismsCancer dataMammalsPredictor of functionHeritability
2020
Analysis of Genetically Regulated Gene Expression Identifies a Prefrontal PTSD Gene, SNRNP35, Specific to Military Cohorts
Huckins L, Chatzinakos C, Breen M, Hartmann J, Klengel T, da Silva Almeida A, Dobbyn A, Girdhar K, Hoffman G, Klengel C, Logue M, Lori A, Maihofer A, Morrison F, Nguyen H, Park Y, Ruderfer D, Sloofman L, van Rooij S, Consortium P, Baker D, Chen C, Cox N, Duncan L, Geyer M, Glatt S, Im H, Risbrough V, Smoller J, Stein D, Yehuda R, Liberzon I, Koenen K, Jovanovic T, Kellis M, Miller M, Bacanu S, Nievergelt C, Buxbaum J, Sklar P, Ressler K, Stahl E, Daskalakis N. Analysis of Genetically Regulated Gene Expression Identifies a Prefrontal PTSD Gene, SNRNP35, Specific to Military Cohorts. Cell Reports 2020, 31: 107716. PMID: 32492425, PMCID: PMC7359754, DOI: 10.1016/j.celrep.2020.107716.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCase-Control StudiesCohort StudiesDexamethasoneDown-RegulationGene Expression RegulationGene Regulatory NetworksGenetic Predisposition to DiseaseHumansLeukocytesMaleMiceMice, Inbred C57BLMilitary PersonnelPrefrontal CortexRepressor ProteinsRibonucleoproteins, Small NuclearRNA InterferenceRNA, Small InterferingStress Disorders, Post-TraumaticConceptsPost-traumatic stress disorderGenetically regulated gene expressionPost-traumatic stress disorder casesDorsolateral prefrontal cortexGene expressionU12 intron splicingPrefrontal cortexStress disorderDeployment stressTranscriptome imputationTissue-specific gene expressionDifferential gene expressionMilitary cohortZNF140U12 intronsGenetic heterogeneityExpression changesFunctional roleExogenous glucocorticoidsPeripheral leukocytesEuropean cohortCortexCohortDisordersExpression
2013
Olfaction and olfactory-mediated behaviour in psychiatric disease models
Huckins L, Logan D, Sánchez-Andrade G. Olfaction and olfactory-mediated behaviour in psychiatric disease models. Cell And Tissue Research 2013, 354: 69-80. PMID: 23604803, DOI: 10.1007/s00441-013-1617-7.Peer-Reviewed Original ResearchConceptsOlfactory-mediated behaviorsPsychiatric diseasesRodent models of psychiatric disordersModels of psychiatric disordersModels of psychiatric diseaseTests of aggressionModel psychiatric diseaseAssociated with neuropsychiatric diseasesRodent modelsBehavioral paradigmsPsychiatric disordersOlfactory memoryExcellent sense of smellOlfactory functionNeuropsychiatric diseasesExperimental paradigmOlfactory dysfunctionAberrant behaviorBehavioral responsesOlfactory subsystemsEthological contextOlfactory componentsSense of smellSensory cuesSocial interaction