2019
Syk-dependent glycolytic reprogramming in dendritic cells regulates IL-1β production to β-glucan ligands in a TLR-independent manner
Thwe PM, Fritz DI, Snyder JP, Smith PR, Curtis KD, O'Donnell A, Galasso NA, Sepaniac LA, Adamik BJ, Hoyt LR, Rodriguez PD, Hogan TC, Schmidt AF, Poynter ME, Amiel E. Syk-dependent glycolytic reprogramming in dendritic cells regulates IL-1β production to β-glucan ligands in a TLR-independent manner. Journal Of Leukocyte Biology 2019, 106: 1325-1335. PMID: 31509298, PMCID: PMC6883127, DOI: 10.1002/jlb.3a0819-207rr.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBeta-GlucansDendritic CellsGlycolysisInterleukin-1betaLectins, C-TypeLigandsMiceMyeloid Differentiation Factor 88NLR Family, Pyrin Domain-Containing 3 ProteinPhosphatidylinositol 3-KinasesProtein Serine-Threonine KinasesProto-Oncogene Proteins c-aktSignal TransductionSyk KinaseToll-Like ReceptorsConceptsTLR-independent mannerDendritic cellsGlucan ligandMetabolic regulationMetabolic reprogramming eventsGlycolytic reprogrammingPyrin domain-containing protein 3 (NLRP3) inflammasome activationActivation of DCsProtein 3 inflammasome activationC-type lectin receptorsImmune effector functionsReprogramming eventsIL-1β productionImmune cell activationInnate immune receptorsFungal microbesMetabolic reprogrammingTyrosine kinaseReprogrammingImmune receptorsCytokine productionIL-1βT cellsInflammasome activationEffector functions
2017
Mitochondrial ROS induced by chronic ethanol exposure promote hyper-activation of the NLRP3 inflammasome
Hoyt LR, Randall MJ, Ather JL, DePuccio DP, Landry CC, Qian X, Janssen-Heininger YM, van der Vliet A, Dixon AE, Amiel E, Poynter ME. Mitochondrial ROS induced by chronic ethanol exposure promote hyper-activation of the NLRP3 inflammasome. Redox Biology 2017, 12: 883-896. PMID: 28463821, PMCID: PMC5413213, DOI: 10.1016/j.redox.2017.04.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineEthanolGene Expression RegulationHumansInflammasomesInterleukin-1betaLeukocytes, MononuclearMacrophagesMiceMitochondriaNLR Family, Pyrin Domain-Containing 3 ProteinReactive Oxygen SpeciesConceptsChronic ethanol exposureEthanol exposureNLRP3 inflammasomeInflammasome activationPro-inflammatory cytokines IL-1βHuman peripheral blood mononuclear cellsPeripheral blood mononuclear cellsLong-term ethanol exposureInflammation-linked diseasesNLRP3 inflammasome hyperactivationAberrant NLRP3 inflammasome activationBlood mononuclear cellsExposure of leukocytesCytokines IL-1βNLRP3 inflammasome activationIL-1β secretionNO scavenger carboxy-PTIOAlcohol use disorderMetabolism of ethanolScavenger carboxy-PTIOMouse macrophage cell lineIL-18IL-1βMononuclear cellsProlonged administration
2016
Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation
Hoyt LR, Ather JL, Randall MJ, DePuccio DP, Landry CC, Wewers MD, Gavrilin MA, Poynter ME. Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation. The Journal Of Immunology 2016, 197: 1322-1334. PMID: 27421477, PMCID: PMC4975963, DOI: 10.4049/jimmunol.1600406.Peer-Reviewed Original ResearchMeSH KeywordsAlcoholsAnimalsBlotting, WesternCells, CulturedEnzyme-Linked Immunosorbent AssayEthanolHumansInflammasomesMiceMice, Inbred C57BLNLR Family, Pyrin Domain-Containing 3 ProteinProtein Tyrosine PhosphatasesConceptsTyrosine phosphorylationPattern recognition receptor complexTyrosine phosphatase inhibitor sodiumPhosphatase inhibitor sodiumCellular tyrosine phosphorylationNLRP3 inflammasome activationApical eventsNLRP3 inflammasomeProtein tyrosineMouse macrophage cell lineCARD (ASC) speck formationApoptosis-associated speck-like proteinMacrophage cell lineIL-1βInflammasome activationSpeck-like proteinReceptor complexMouse bone marrow-derived dendritic cellsBone marrow-derived dendritic cellsPhosphorylationMarrow-derived dendritic cellsProinflammatory cytokines IL-1βCell linesInhibitory effectCaspase-1 cleavage