2017
Transcriptional signatures of schizophrenia in hiPSC-derived NPCs and neurons are concordant with post-mortem adult brains
Hoffman GE, Hartley BJ, Flaherty E, Ladran I, Gochman P, Ruderfer DM, Stahl EA, Rapoport J, Sklar P, Brennand KJ. Transcriptional signatures of schizophrenia in hiPSC-derived NPCs and neurons are concordant with post-mortem adult brains. Nature Communications 2017, 8: 2225. PMID: 29263384, PMCID: PMC5738408, DOI: 10.1038/s41467-017-02330-5.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAntigens, SurfaceAutopsyBrainCase-Control StudiesChildDNA Copy Number VariationsFemaleHumansInduced Pluripotent Stem CellsLinear ModelsMaleNanog Homeobox ProteinNestinNeural Stem CellsNeuronsOctamer Transcription Factor-3ProteoglycansRNA, MessengerSchizophreniaSequence Analysis, RNASOXB1 Transcription FactorsStage-Specific Embryonic AntigensSynapsinsTranscriptomeYoung Adult
2015
A guide to generating and using hiPSC derived NPCs for the study of neurological diseases.
Topol A, Tran N, Brennand K. A guide to generating and using hiPSC derived NPCs for the study of neurological diseases. Journal Of Visualized Experiments 2015, e52495. PMID: 25742222, PMCID: PMC4354663, DOI: 10.3791/52495.Peer-Reviewed Original ResearchConceptsNeural progenitor cellsHiPSC neural progenitor cellsRapid genetic screeningPluripotent stem cellsCellular phenotypesDevelopmental eventsMolecular consequencesGene expressionNeurological diseasesFunctional neuronsStem cellsProgenitor cellsOnset of symptomsMolecular factorsFurther differentiationPost-mortem studiesDisease initiationGenetic screeningOxidative stressSymptom onsetDisease progressionHealthy controlsDiseaseCellsPatients