2003
The evolving roles of cell surface proteases in health and disease: Implications for developmental, adaptive, inflammatory, and neoplastic processes
Madri JA. The evolving roles of cell surface proteases in health and disease: Implications for developmental, adaptive, inflammatory, and neoplastic processes. Current Topics In Developmental Biology 2003, 54: 391-410. PMID: 12696757, DOI: 10.1016/s0070-2153(03)54016-9.Peer-Reviewed Original ResearchConceptsCell surface proteolysisCell surface proteaseSurface proteolysisSurface proteaseDiversity of enzymesExtracellular matrix remodelingMaintenance of homeostasisBiological functionsMatrix remodelingAdaptive responseNormal homeostasisProteolytic responseCell proliferationProteolysisProtease activityEnzyme systemGeneral importanceHomeostasisProteaseCell attractionVascular systemDegenerative diseasesImportant processNeoplastic processComplement system
2001
PECAM‐1 shedding during apoptosis generates a membrane‐anchored truncated molecule with unique signaling characteristics
ILAN N, MOHSENIN A, CHEUNG L, MADRI J. PECAM‐1 shedding during apoptosis generates a membrane‐anchored truncated molecule with unique signaling characteristics. The FASEB Journal 2001, 15: 362-372. PMID: 11156952, DOI: 10.1096/fj.00-0372com.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid Chloromethyl KetonesAnimalsAntigens, CDApoptosisBlood PlateletsCaspasesCattleCell DivisionCell LineCell MembraneCells, CulturedColonic NeoplasmsCulture MediaDipeptidesEndothelium, VascularEnzyme InhibitorsHumansPlatelet Endothelial Cell Adhesion Molecule-1Sequence DeletionSignal TransductionTransfectionTumor Cells, CulturedUmbilical VeinsConceptsFull-length PECAM-1Signal transduction cascadeSignal transduction eventsCaspase-8 cleavageCell proliferationPECAM-1SW480 colon carcinoma cellsCaspase substratesSHP-2Transduction cascadeTransduction eventsGrowth factor receptorCell adhesion moleculeGene constructsCell surface moleculesColon carcinoma cellsSoluble proteinStable expressionCell deathCulture mediumMatrix metalloproteinaseCell surfaceJNK phosphorylationUnique functionFactor receptor
1998
The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis.
Graesser D, Mahooti S, Haas T, Davis S, Clark R, Madri J. The interrelationship of alpha4 integrin and matrix metalloproteinase-2 in the pathogenesis of experimental autoimmune encephalomyelitis. Laboratory Investigation 1998, 78: 1445-58. PMID: 9840619.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDCell AdhesionCell MembraneCell MovementCells, CulturedClone CellsEncephalomyelitis, Autoimmune, ExperimentalEndothelium, VascularGelatinasesIntegrin alpha4Matrix Metalloproteinase 14Matrix Metalloproteinase 2Matrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesMiceMice, Inbred StrainsProtease InhibitorsTissue Inhibitor of Metalloproteinase-2T-LymphocytesTransfectionConceptsExperimental autoimmune encephalomyelitisT cell clonesRecombinant vascular cell adhesion molecule-1Autoreactive T cell clonesAlpha4 integrinsMMP-2EAE inductionAutoimmune encephalomyelitisMyelin basic protein-reactive T cell clonesDisease processMatrix metalloproteinaseVascular cell adhesion molecule-1Cell adhesion molecule-1Human multiple sclerosisSusceptible mouse strainsAdhesion molecule-1T cell transmigrationMatrix metalloproteinase-2Microvascular endothelial cellsAlpha4 integrin expressionExpression of alpha4Adoptive transferMultiple sclerosisMouse modelEndothelial cell layer
1996
Regulation of human colonic cell line proliferation and phenotype by sodium butyrate
Basson M, Turowski G, Rashid Z, Hong F, Madri J. Regulation of human colonic cell line proliferation and phenotype by sodium butyrate. Digestive Diseases And Sciences 1996, 41: 1986-1993. PMID: 8888712, DOI: 10.1007/bf02093601.Peer-Reviewed Original ResearchConceptsBrush border enzyme activitiesSerial cell countsCell line proliferationHuman colonic cellsColonic butyrateApparent protective actionColon carcinogenesisMalignant behaviorCell countButyrate effectMucosal differentiationColonic cellsProtective actionSodium butyrateCaco-2Surface expressionCollagen ICollagen IVDietary fiberProliferationRelevant concentrationsCarcinogenesisCultured cellsMotilityMatrix proteins
1995
Expression of Transforming Growth Factor Type III Receptor in Vascular Endothelial Cells Increases Their Responsiveness to Transforming Growth Factor β2 ∗
Sankar S, Mahooti-Brooks N, Centrella M, McCarthy T, Madri J. Expression of Transforming Growth Factor Type III Receptor in Vascular Endothelial Cells Increases Their Responsiveness to Transforming Growth Factor β2 ∗. Journal Of Biological Chemistry 1995, 270: 13567-13572. PMID: 7768960, DOI: 10.1074/jbc.270.22.13567.Peer-Reviewed Original ResearchConceptsTGF beta 2Type II receptorBovine aortic endothelial cellsTGF beta 1Receptor proteinType III receptorII receptorsBeta 2TGF-beta type III receptorBeta 1Serine-threonine kinaseInhibitor-1 proteinPlasminogen activator inhibitor-1 proteinEndothelial cellsTGF beta sGrowth factor betaGrowth factor β2Inhibition of migrationVascular endothelial cellsSignaling capacityType IAortic endothelial cellsReceptor cDNAReceptor complexProteinGerminal matrix microvascular maturation correlates inversely with the risk period for neonatal intraventricular hemorrhage
Ment L, Stewart W, Ardito T, Madri J. Germinal matrix microvascular maturation correlates inversely with the risk period for neonatal intraventricular hemorrhage. Brain Research 1995, 84: 142-149. PMID: 7720213, DOI: 10.1016/0165-3806(94)00168-y.Peer-Reviewed Original ResearchConceptsNeonatal intraventricular hemorrhageIntraventricular hemorrhagePostnatal dayRisk periodDay 1Blood-brain barrierNewborn beagle pupsTenth postnatal dayEndothelial cell areaBasal lamina depositionPerinatal inductionPreterm neonatesBasement membrane areaWeeks' gestationGestational agePostnatal ageGerminal matrixBeagle pupsElectron microscopic examinationLuminal areaTight junction lengthWhite matterDay 10Time pointsBeagle model
1992
Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration
Schimmenti L, Yan H, Madri J, Albelda S. Platelet endothelial cell adhesion molecule, PECAM‐1, modulates cell migration. Journal Of Cellular Physiology 1992, 153: 417-428. PMID: 1429859, DOI: 10.1002/jcp.1041530222.Peer-Reviewed Original ResearchConceptsCell-cell adhesionCell-cell adhesion moleculesCell-cell contactPlatelet endothelial cell adhesion molecule-1Cell migrationIntegral membrane proteinsAdhesion moleculesPECAM-1 moleculesControl cellsCell surface adhesion moleculesModulates cell migrationNeomycin resistance geneEndothelial cell migrationMembrane proteinsCell adhesion moleculeEndothelial cellsExtracellular domainSurface adhesion moleculesNull cellsNIH/3T3 cellsResistance genesExtracellular matrixTransfectantsAdjacent cellsMigration assays
1990
A 48 kDa collagen-binding phosphoprotein isolated from bovine aortic endothelial cells interacts with the collagenous domain, but not the globular domain, of collagen type IV
Yannariello-Brown J, Madri J. A 48 kDa collagen-binding phosphoprotein isolated from bovine aortic endothelial cells interacts with the collagenous domain, but not the globular domain, of collagen type IV. Biochemical Journal 1990, 265: 383-392. PMID: 2154186, PMCID: PMC1136898, DOI: 10.1042/bj2650383.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaCattleCell MembraneCells, CulturedChromatography, AffinityChymotrypsinCollagenElectrophoresis, Gel, Two-DimensionalElectrophoresis, Polyacrylamide GelEndothelium, VascularFluorescent Antibody TechniqueImmune SeraMolecular WeightPeptide MappingPhosphoproteinsReceptors, Cell SurfaceReceptors, CollagenConceptsBovine aortic endothelial cellsSDS/PAGENon-equilibrium pH gel electrophoresisCollagen type IVCollagenous domainTwo-dimensional gel systemAortic endothelial cellsAdhesion assaysCell surface populationIndirect immunofluorescence experimentsGlobular NC1 domainCell surface labelingCollagen-binding proteinsChymotryptic peptide mapsEndothelial cellsGlobular domainIntracellular transportMinor isoformIndividual isoformsImmunofluorescence experimentsMolecular massGolgi regionCollagenous regionCell surfacePeptide maps
1987
Dependence on pH of polarized sorting of secreted proteins
Caplan M, Stow J, Newman A, Madri J, Anderson H, Farquhar M, Palade G, Jamieson J. Dependence on pH of polarized sorting of secreted proteins. Nature 1987, 329: 632-635. PMID: 2821405, DOI: 10.1038/329632a0.Peer-Reviewed Original ResearchConceptsSecretory proteinsMDCK cellsDifferent protein compositionsBasolateral cell surfacePolarized sortingMembrane proteinsBasolateral domainDefault pathwayPlasma membraneAcidic intracellular compartmentsIntracellular compartmentsProtein compositionMembrane componentsCell surfaceBasement membrane componentsProteinSecrete lamininDistinct setsRenal tubule cellsEpithelial cellsActive sortingBasolateral compartmentCellsSecretory productsSpecific regionsAortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species.
Keller R, Silbert J, Furthmayr H, Madri J. Aortic endothelial cell proteoheparan sulfate. I. Isolation and characterization of plasmamembrane-associated and extracellular species. American Journal Of Pathology 1987, 128: 286-98. PMID: 3039849, PMCID: PMC1899622.Peer-Reviewed Original ResearchConceptsDaltons apparent molecular weightProteoheparan sulfate speciesHeparan sulfate chainsApparent molecular weightHS IBovine aortic endothelial cellsSulfate chainsCultured bovine aortic endothelial cellsCore proteinAortic endothelial cellsCell surface localizationPlasma membrane fractionEndothelial cellsPulse-chase experimentsCsCl density centrifugationThird speciesPlasma membraneSubcellular fractionationExtracellular speciesSpecialized functionsMatrix localizationSurface localizationMembrane fractionSulfate biosynthesisMedium species