2023
Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Huang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.Peer-Reviewed Original ResearchConceptsBiofilm matrix exopolysaccharideFacilitate host colonizationVibrio cholerae biofilmsΒ-propeller domainMatrix exopolysaccharideModular domainsHost colonizationRedundant rolesDistinct functionsAbiotic surfacesAdhesive proteinsHost surfaceHuman pathogensVibrio choleraeAdhesinsBacterial biofilmsHost tissuesColonization modelColonizationBAP1BiofilmsPathogensAntibiotic resistanceRBMCDomainNew Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology
Tai J, Ferrell M, Yan J, Waters C. New Insights into Vibrio cholerae Biofilms from Molecular Biophysics to Microbial Ecology. Advances In Experimental Medicine And Biology 2023, 1404: 17-39. PMID: 36792869, PMCID: PMC10726288, DOI: 10.1007/978-3-031-22997-8_2.Peer-Reviewed Original ResearchConceptsV. cholerae biofilmsBacterial signal transduction networkSignal transduction networksBiofilm formationVibrio cholerae biofilmsKey model systemV. choleraeEcological roleMicrobial ecologyTransduction networksBiofilm maturationEnvironmental survivalCholera pathogenesisMolecular biophysicsRegulatory systemVibrio choleraeEl Tor biotypeBacterial surface attachmentRecent insightsModel systemBiofilmsLife cycleSurface attachmentCholeraeCentral function
2022
Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement
Barrasso K, Chac D, Debela M, Geigel C, Steenhaut A, Seda A, Dunmire C, Harris J, Larocque R, Midani F, Qadri F, Yan J, Weil A, Ng W. Impact of a human gut microbe on Vibrio cholerae host colonization through biofilm enhancement. ELife 2022, 11: e73010. PMID: 35343438, PMCID: PMC8993218, DOI: 10.7554/elife.73010.Peer-Reviewed Original ResearchConceptsGut microbesOutcome of infectionHuman intestinal microbiotaMurine small intestineUninfected personsEnteric infectionsDisease outcomeIntestinal microbiotaSmall intestineHuman gut microbesCommensal bacteriumEtiological agentOutcomesDiarrheal disease choleraInfectionDisease choleraRecent studiesAir-liquid interfaceBacterium altersBiofilm enhancementStoolIntestineBacterial Surface Detachment during Nebulization with Contaminated Reusable Home Nebulizers
Harris JC, Collins MS, Huang PH, Schramm CM, Nero T, Yan J, Murray TS. Bacterial Surface Detachment during Nebulization with Contaminated Reusable Home Nebulizers. Microbiology Spectrum 2022, 10: e02535-21. PMID: 35107362, PMCID: PMC8809330, DOI: 10.1128/spectrum.02535-21.Peer-Reviewed Original ResearchConceptsAlbuterol nebulizationNebulized therapyHome nebulizersCystic fibrosisChronic respiratory diseasesAerosolized medicationsLung infectionCF patientsRespiratory diseasePathogenic bacteriaPatientsNext Generation ImpactorClinical isolatesMedicationsLungPari LCTherapyAnonymous patientsClinical bacteriaNebulizationNebulizerAerosolizationPathogenic microbesDetachmentSubset of bacteria
2018
Bacterial Biofilm Material Properties Enable Removal and Transfer by Capillary Peeling
Yan J, Moreau A, Khodaparast S, Perazzo A, Feng J, Fei C, Mao S, Mukherjee S, Košmrlj A, Wingreen NS, Bassler BL, Stone HA. Bacterial Biofilm Material Properties Enable Removal and Transfer by Capillary Peeling. Advanced Materials 2018, 30: e1804153. PMID: 30368924, PMCID: PMC8865467, DOI: 10.1002/adma.201804153.Peer-Reviewed Original ResearchConceptsBiofilm removal strategiesMaterial propertiesBiofouling problemsInterfacial propertiesSurface foulingRheological characterizationIndustrial operationsMechanical protectionSurface analysisBiofilm-forming bacterial speciesMultiple surfacesBiofilm-dwelling cellsVibrio cholerae biofilmsSurface-attached communitiesSurfaceRemoval strategiesPropertiesFouling
2016
Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging
Yan J, Sharo AG, Stone HA, Wingreen NS, Bassler BL. Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5337-e5343. PMID: 27555592, PMCID: PMC5018804, DOI: 10.1073/pnas.1611494113.Peer-Reviewed Original ResearchConceptsV. cholerae biofilmsSingle-cell live imagingSurface-associated bacterial communitiesVibrio cholerae biofilmsSingle-cell technologiesSingle founder cellRod-shaped bacteriaFounder cellsSingle geneBacterial communitiesLive imagingBiofilm architectureBiofilm componentsBiofilm structureCell heterogeneityIndividual cellsMatrix labelingMature biofilmsArt microscopy techniquesCompetition analysisBiofilmsSingle-cell resolution imagingGenesGrowth programCells