2023
Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization
Huang X, Nero T, Weerasekera R, Matej K, Hinbest A, Jiang Z, Lee R, Wu L, Chak C, Nijjer J, Gibaldi I, Yang H, Gamble N, Ng W, Malaker S, Sumigray K, Olson R, Yan J. Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization. Nature Communications 2023, 14: 2104. PMID: 37055389, PMCID: PMC10102183, DOI: 10.1038/s41467-023-37660-0.Peer-Reviewed Original ResearchConceptsBiofilm matrix exopolysaccharideFacilitate host colonizationVibrio cholerae biofilmsΒ-propeller domainMatrix exopolysaccharideModular domainsHost colonizationRedundant rolesDistinct functionsAbiotic surfacesAdhesive proteinsHost surfaceHuman pathogensVibrio choleraeAdhesinsBacterial biofilmsHost tissuesColonization modelColonizationBAP1BiofilmsPathogensAntibiotic resistanceRBMCDomain
2017
Extracellular-matrix-mediated osmotic pressure drives Vibrio cholerae biofilm expansion and cheater exclusion
Yan J, Nadell CD, Stone HA, Wingreen NS, Bassler BL. Extracellular-matrix-mediated osmotic pressure drives Vibrio cholerae biofilm expansion and cheater exclusion. Nature Communications 2017, 8: 327. PMID: 28835649, PMCID: PMC5569112, DOI: 10.1038/s41467-017-00401-1.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsBiofilmsExtracellular MatrixMicroscopy, ConfocalMutationOsmotic PressureVibrio choleraeConceptsBiofilm-forming bacterial speciesSurface-attached communitiesVibrio choleraeActive cell growthModel organismsMatrix-producing cellsBacterial lifeNutrient uptakeEnvironmental perturbationsBacterial speciesBacterial cellsPlanktonic cellsSubmerged biofilmsExtracellular matrixCell growthMatrix productionOsmotic pressureBiofilm growthBiofilmsCholeraeCellsPhysical exclusionMatrix crosslinkingOsmotic swellingGrowth
2016
Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging
Yan J, Sharo AG, Stone HA, Wingreen NS, Bassler BL. Vibrio cholerae biofilm growth program and architecture revealed by single-cell live imaging. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5337-e5343. PMID: 27555592, PMCID: PMC5018804, DOI: 10.1073/pnas.1611494113.Peer-Reviewed Original ResearchMeSH KeywordsBacterial AdhesionBacterial ProteinsBiofilmsCell ProliferationHumansSingle-Cell AnalysisVibrio choleraeConceptsV. cholerae biofilmsSingle-cell live imagingSurface-associated bacterial communitiesVibrio cholerae biofilmsSingle-cell technologiesSingle founder cellRod-shaped bacteriaFounder cellsSingle geneBacterial communitiesLive imagingBiofilm architectureBiofilm componentsBiofilm structureCell heterogeneityIndividual cellsMatrix labelingMature biofilmsArt microscopy techniquesCompetition analysisBiofilmsSingle-cell resolution imagingGenesGrowth programCells