2024
Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS).
Weerasekera R, Moreau A, Huang X, Nam K, Hinbest A, Huynh Y, Liu X, Ashwood C, Pepi L, Paulson E, Cegelski L, Yan J, Olson R. Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS). PLOS Pathogens 2024, 20: e1012750. PMID: 39621768, DOI: 10.1371/journal.ppat.1012750.Peer-Reviewed Original ResearchVibrio polysaccharideBiofilm dispersalHuman pathogen Vibrio choleraeV. cholerae biofilmsPathogen Vibrio choleraeBiofilm disruption activityFormation of biofilmsVibrio coralliilyticusPolysaccharide lyasesLyase mechanismV. choleraeVibrio choleraeBiofilm matrixPathogenic bacteriaRbmBAntibacterial enzymesSecreted macromoleculesSolid-state NMRGlycolytic enzymesFluorescence-based biochemical assayBiofilmBiochemical assaysEnzymeSecreted glycosidasesDouble bond
2017
Environmental fluctuation governs selection for plasticity in biofilm production
Yan J, Nadell CD, Bassler BL. Environmental fluctuation governs selection for plasticity in biofilm production. The ISME Journal: Multidisciplinary Journal Of Microbial Ecology 2017, 11: 1569-1577. PMID: 28338673, PMCID: PMC5520152, DOI: 10.1038/ismej.2017.33.Peer-Reviewed Original ResearchConceptsHuman pathogen Vibrio choleraePlanktonic growthFree-swimming stateBiofilm formationPathogen Vibrio choleraeSurface-attached communitiesWild-type V. choleraeNon-producing mutantsBiofilm growth modeCell-cell interactionsSuch flexible strategiesBiofilm productionHabitat turnoverPhenotypic flexibilityCompetitive fitnessMolecular programsCompetitive abilityEnvironmental fluctuationsPlanktonic phaseWild typeBiofilm conditionsPlanktonic conditionsEcological conditionsNutrient accessBiofilm producers