2024
Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS).
Weerasekera R, Moreau A, Huang X, Nam K, Hinbest A, Huynh Y, Liu X, Ashwood C, Pepi L, Paulson E, Cegelski L, Yan J, Olson R. Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS). PLOS Pathogens 2024, 20: e1012750. PMID: 39621768, DOI: 10.1371/journal.ppat.1012750.Peer-Reviewed Original ResearchVibrio polysaccharideBiofilm dispersalHuman pathogen Vibrio choleraeV. cholerae biofilmsPathogen Vibrio choleraeBiofilm disruption activityFormation of biofilmsVibrio coralliilyticusPolysaccharide lyasesLyase mechanismV. choleraeVibrio choleraeBiofilm matrixPathogenic bacteriaRbmBAntibacterial enzymesSecreted macromoleculesSolid-state NMRGlycolytic enzymesFluorescence-based biochemical assayBiofilmBiochemical assaysEnzymeSecreted glycosidasesDouble bond
2022
Molecular Mechanism of Vibrio cholerae biofilm adhesion
Weerasekera R, Hinbest A, Nero T, Huang X, Yan J, Olson R. Molecular Mechanism of Vibrio cholerae biofilm adhesion. The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.l7499.Peer-Reviewed Original ResearchΒ-prism domainV. cholerae biofilmsBiofilm matrixAbiotic surfacesCommunity of microorganismsComplex N-glycansΒ-propeller domainHost colonizationSequence identityAdhesion proteinsDistinct functionsMolecular mechanismsN-glycansBacterial cellsFunctional assaysHost surfaceVirulence factorsVibrio choleraeProteinMain proteinsV. choleraeSpecific adhesion moleculesBiofilmsBAP1Cholerae