2023
Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation
Takasugi T, Gu P, Liang F, Staco I, Chang S. Pot1b −/− tumors activate G-quadruplex-induced DNA damage to promote telomere hyper-elongation. Nucleic Acids Research 2023, 51: 9227-9247. PMID: 37560909, PMCID: PMC10516629, DOI: 10.1093/nar/gkad648.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA DamageDNA-Binding ProteinsHumansMiceReplication Protein ASarcomaShelterin ComplexTelomereTelomere-Binding ProteinsConceptsDNA damage responseDamage responseReplication protein A (RPA) complexDependent DNA damage responseTelomere length homeostasisTelomere maintenance mechanismLength homeostasisTelomerase recruitmentPOT1 proteinsHuman POT1Mouse genomeLength maintenanceFunction disruptsReplicative immortalityTelomeresPOT1 mutationsDNA damageHuman cancersLonger telomeresPOT1bMaintenance mechanismsSerial transplantationA complexesSimilar mechanismMutations
2019
DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response
Liang F, Miller AS, Longerich S, Tang C, Maranon D, Williamson EA, Hromas R, Wiese C, Kupfer GM, Sung P. DNA requirement in FANCD2 deubiquitination by USP1-UAF1-RAD51AP1 in the Fanconi anemia DNA damage response. Nature Communications 2019, 10: 2849. PMID: 31253762, PMCID: PMC6599204, DOI: 10.1038/s41467-019-10408-5.Peer-Reviewed Original ResearchConceptsFANCD2 deubiquitinationDNA damage response pathwayFanconi anemiaUSP1-UAF1 complexDNA damage toleranceDNA damage responseDamage response pathwayImportance of DNAGenome repairDamage responseResponse pathwaysUAF1DNA requirementsCellular settingsFA pathwayDNA bindingDownstream eventsDeubiquitinationRAD51AP1DNA crosslinksBiochemical systemsBone marrow failureChromosomal lesionsMultigenic diseasesMultifaceted role
2016
Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex
Liang F, Longerich S, Miller AS, Tang C, Buzovetsky O, Xiong Y, Maranon DG, Wiese C, Kupfer GM, Sung P. Promotion of RAD51-Mediated Homologous DNA Pairing by the RAD51AP1-UAF1 Complex. Cell Reports 2016, 15: 2118-2126. PMID: 27239033, PMCID: PMC5381662, DOI: 10.1016/j.celrep.2016.05.007.Peer-Reviewed Original ResearchConceptsHomologous recombinationFanconi anemia DNA damage response pathwayDNA damage response pathwaySmall ubiquitin-like modifierDNA damage hypersensitivityHomologous DNA pairingSUMO-interacting motifDamage response pathwayUbiquitin-like modifierFANCD2 ubiquitinationGenome maintenanceComplex formationNucleoprotein intermediatesRAD51 recombinaseDNA pairingProtein complexesBinds DNAAccessory factorsResponse pathwaysHomologous DNADNA repairLike domainUAF1RAD51RAD51AP1
2015
NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability
Parplys AC, Zhao W, Sharma N, Groesser T, Liang F, Maranon DG, Leung SG, Grundt K, Dray E, Idate R, Østvold AC, Schild D, Sung P, Wiese C. NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability. Nucleic Acids Research 2015, 43: 9817-9834. PMID: 26323318, PMCID: PMC4787752, DOI: 10.1093/nar/gkv859.Peer-Reviewed Original ResearchConceptsHomologous recombinationDNA replication fork progressionChromatin-associated proteinsVertebrate-specific proteinReplication fork progressionDNA damage responseDNA repair pathwaysNew replication originsNumber of proteinsExtensive sequence homologyImpairs DNA repairGenome stabilityLimited functional dataChromosome stabilityFork progressionDamage responseReplication originsHR pathwayPhenotypic effectsDNA repairRepair pathwaysSequence homologyTumor suppressionSame DNANUCKS1