2021
The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores
Wu Z, Dharan N, McDargh ZA, Thiyagarajan S, O'Shaughnessy B, Karatekin E. The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores. ELife 2021, 10: e68215. PMID: 34190041, PMCID: PMC8294851, DOI: 10.7554/elife.68215.Peer-Reviewed Original ResearchFisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues CDA, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams ND, Lin C, Wingreen NS, Rudner DZ, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. PLOS Biology 2021, 19: e3001314. PMID: 34185788, PMCID: PMC8274934, DOI: 10.1371/journal.pbio.3001314.Peer-Reviewed Original ResearchConceptsMembrane fissionMembrane fission machineryMother cell membraneNegative-curvature lipidsMother cell cytoplasmUnique membrane topologyFission machineryMembrane scissionMembrane topologyLipid microdomainsMembrane necksFission proteinsLipid bindingMembrane curvatureFilamentous cellsBacillus subtilisFisBProtein BCell cytoplasmAcidic lipidsCell membraneCurvature sensingImmobile clustersIntrinsic abilityBacteria
2016
Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores
Stratton BS, Warner JM, Wu Z, Nikolaus J, Wei G, Wagnon E, Baddeley D, Karatekin E, O’Shaughnessy B. Cholesterol Increases the Openness of SNARE-Mediated Flickering Fusion Pores. Biophysical Journal 2016, 110: 1538-1550. PMID: 27074679, PMCID: PMC4833774, DOI: 10.1016/j.bpj.2016.02.019.Peer-Reviewed Original ResearchMeSH KeywordsCholesterolKineticsMembrane FusionMicroscopy, FluorescenceModels, MolecularProtein ConformationSNARE ProteinsUnilamellar LiposomesConceptsT-SNAREsFusion poreSoluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteinsLipid bilayer propertiesFactor attachment protein receptor proteinsN-ethylmaleimide-sensitive factor attachment protein receptor proteinsTotal internal reflection fluorescence microscopyCognate t-SNAREsFusion pore dynamicsPhysiological cholesterol levelsProtein receptor proteinsFlickering fusion poresReflection fluorescence microscopyIndividual fusion eventsV-SNAREBilayer propertiesFusion eventsPore flickeringReceptor proteinFluorescence microscopySmall unilamellar vesiclesLipid compositionFusion siteSnareExocytotic release