2009
Protein-protein interactions among ion channels regulate ion transport in the kidney.
Boulpaep E. Protein-protein interactions among ion channels regulate ion transport in the kidney. Bulletin Et Mémoires De L'Académie Royale De Médecine De Belgique 2009, 164: 133-41; discussion 141-2. PMID: 20120088.Peer-Reviewed Original ResearchConceptsAMP kinaseProtein CFTRCFTR channel gatingMembrane transport proteinsProtein-protein interactionsMembrane-attached proteinsSerine-threonine kinaseRegulation of transportKir 1.1Mg-ATPIon transportExtracellular agonistsMembrane proteinsTransport proteinsChannel gatingIntracellular ATP concentrationIntracellular messengerMembrane receptorsCFTRMetabolic signalsIon channelsChloride channelsEpithelial ion transportDirect interactionRenal K secretion
2006
CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney
Lu M, Leng Q, Egan ME, Caplan MJ, Boulpaep EL, Giebisch GH, Hebert SC. CFTR is required for PKA-regulated ATP sensitivity of Kir1.1 potassium channels in mouse kidney. Journal Of Clinical Investigation 2006, 116: 797-807. PMID: 16470247, PMCID: PMC1361349, DOI: 10.1172/jci26961.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsCurcuminCyclic AMP-Dependent Protein KinasesCystic Fibrosis Transmembrane Conductance RegulatorHydrogen-Ion ConcentrationKidneyMiceMice, Inbred C57BLMice, Inbred CFTRMice, TransgenicMutationOocytesPatch-Clamp TechniquesPotassium Channels, Inwardly RectifyingXenopus laevisConceptsFunctional switchCystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channelATP sensitivityEffects of CFTRThick ascending limbPotential physiological rolePKA activityRenal K channelsCystic fibrosisPhysiological roleSecretory channelsK channelsRenal tubule epithelial cellsApical membraneCFTRDeltaF508 mutationDistal nephron segmentsCl- channelsK homeostasisTubule epithelial cellsEpithelial cellsTAL cellsPotassium channelsK handlingGlibenclamide sensitivity
2004
Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension
Kahle KT, MacGregor GG, Wilson FH, Van Hoek AN, Brown D, Ardito T, Kashgarian M, Giebisch G, Hebert SC, Boulpaep EL, Lifton RP. Paracellular Cl- permeability is regulated by WNK4 kinase: Insight into normal physiology and hypertension. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 14877-14882. PMID: 15465913, PMCID: PMC522037, DOI: 10.1073/pnas.0406172101.Peer-Reviewed Original ResearchConceptsPseudohypoaldosteronism type IIPHAII-mutant WNK4Paracellular fluxPotent antihypertensive agentTight junction proteinsTight junctionsAntihypertensive agentsParacellular ion fluxPharmacologic propertiesTight junction structureTranscellular transportersWild-type WNK4Normal physiologyHypertensionTransepithelial resistanceWNK signalingKidney epitheliumTight junction formationParacellular pathwayWNK4Effect of WNK4EpitheliumType IIWNK4 kinaseHomeostasisCharacteristics of renal Rhbg as an NH4+ transporter
Nakhoul N, DeJong H, Abdulnour-Nakhoul S, Boulpaep E, Hering-Smith K, Hamm L. Characteristics of renal Rhbg as an NH4+ transporter. American Journal Of Physiology. Renal Physiology 2004, 288: f170-f181. PMID: 15353405, DOI: 10.1152/ajprenal.00419.2003.Peer-Reviewed Original Research
1999
Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney
Schmitt B, Biemesderfer D, Romero M, Boulpaep E, Boron W. Immunolocalization of the electrogenic Na+- HCO 3 − cotransporter in mammalian and amphibian kidney. American Journal Of Physiology 1999, 276: f27-f38. PMID: 9887077, DOI: 10.1152/ajprenal.1999.276.1.f27.Peer-Reviewed Original ResearchConceptsRat kidney NBCProximal tubulesDistal tubulesIndirect immunofluorescence microscopyEarly distal tubuleLate distal tubuleControl fusion proteinHCO-3 reabsorptionHCO-3 cotransporterRenal proximal tubulesBasolateral labelingImmunofluorescence microscopyRenal microsomesGuinea pigsRat kidneyHEK-293 cellsKidneyPreabsorption experimentsBasolateral stainingRabbit kidneyControl oocytesFusion proteinRatsProtein levelsNBC protein
1997
Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter
Romero M, Hediger M, Boulpaep E, Boron W. Expression cloning and characterization of a renal electrogenic Na+ /HCO3− cotransporter. Nature 1997, 387: 409-413. PMID: 9163427, DOI: 10.1038/387409a0.Peer-Reviewed Original ResearchConceptsPotential membrane-spanning domainsMembrane-spanning domainsElectrogenic sodium bicarbonate cotransporterBinding of inhibitorsSodium bicarbonate cotransporterAnimal cellsBicarbonate transportersElectrogenic transportersSuccessful cloningExpression cloningComplementary DNAPrincipal regulatorAmino acidsBicarbonate cotransporterProtein purificationFunctional familiesXenopus oocytesMolecular informationTransportersCloningReproductive systemSalamander Ambystoma tigrinumProteinAmbystoma tigrinumIntracellular pH
1989
The electrogenic Na/HCO3 cotransporter
Boron W, Boulpaep E. The electrogenic Na/HCO3 cotransporter. Kidney International 1989, 36: 392-402. PMID: 2687566, DOI: 10.1038/ki.1989.208.Peer-Reviewed Original Research
1977
The effect of organic solutes on proximal tubular fluid absorption in Necturus kidney [proceedings].
Boulpaep E, Forster J, Steels P. The effect of organic solutes on proximal tubular fluid absorption in Necturus kidney [proceedings]. Archives Of Physiology And Biochemistry 1977, 85: 623-4. PMID: 72543.Peer-Reviewed Original Research
1976
Electrical phenomena in the nephron
Boulpaep E. Electrical phenomena in the nephron. Kidney International 1976, 9: 88-102. PMID: 940266, DOI: 10.1038/ki.1976.14.Peer-Reviewed Original ResearchConceptsSingle barrierAvailable experimental dataIon movementElectrical conductanceElectrical phenomenaSingle cell membraneSingle membrane levelElectric currentElectrical propertiesPotential differenceExperimental dataDiffusion barrierDistinctive physical propertiesElectrical characteristicsPhysical propertiesIndividual cell membranesDiffusion boundariesElectrical behaviorPotential gradientMembrane approachConjugate forcesBiological membranesElectrochemical potential gradientTransport processesMicroscopic level