2014
Probing the effect of promoters on noise in gene expression using thousands of designed sequences
Sharon E, van Dijk D, Kalma Y, Keren L, Manor O, Yakhini Z, Segal E. Probing the effect of promoters on noise in gene expression using thousands of designed sequences. Genome Research 2014, 24: 1698-1706. PMID: 25030889, PMCID: PMC4199362, DOI: 10.1101/gr.168773.113.Peer-Reviewed Original ResearchConceptsExpression noiseGene expressionMean expression levelTranscription factorsPromoter sequencesExpression levelsGene expression noisePromoter DNA sequencesMore transcription factorsNucleosome-disfavoring sequencesHigher expression noiseDifferent promoter sequencesNonspecific DNA bindingOne-dimensional slidingCellular functionsHigh-throughput methodNative promoterDNA sequencesDNA bindingSynthetic promotersPromoterIdentical cellsTarget siteSequence
2013
Two DNA-encoded strategies for increasing expression with opposing effects on promoter dynamics and transcriptional noise
Dadiani M, van Dijk D, Segal B, Field Y, Ben-Artzi G, Raveh-Sadka T, Levo M, Kaplow I, Weinberger A, Segal E. Two DNA-encoded strategies for increasing expression with opposing effects on promoter dynamics and transcriptional noise. Genome Research 2013, 23: 966-976. PMID: 23403035, PMCID: PMC3668364, DOI: 10.1101/gr.149096.112.Peer-Reviewed Original ResearchConceptsPromoter dynamicsExpression variabilityPromoter transitionsSingle-cell time-lapse microscopyInactive stateSequence changesNucleosome-disfavoring sequencesCis-regulatory elementsProcess of transcriptionActive stateNumber of transcriptsTime-lapse microscopyCell populationsTranscriptional noiseTranscriptional dynamicsSite resultsTranscription factorsDNA sequencesGene expressionMean expressionIdentical populationsIndividual cellsSequence resultsExpression levelsTranscripts