2019
Glutathione deficiency-elicited reprogramming of hepatic metabolism protects against alcohol-induced steatosis
Chen Y, Manna SK, Golla S, Krausz KW, Cai Y, Garcia-Milian R, Chakraborty T, Chakraborty J, Chatterjee R, Thompson DC, Gonzalez FJ, Vasiliou V. Glutathione deficiency-elicited reprogramming of hepatic metabolism protects against alcohol-induced steatosis. Free Radical Biology And Medicine 2019, 143: 127-139. PMID: 31351176, PMCID: PMC6848780, DOI: 10.1016/j.freeradbiomed.2019.07.025.Peer-Reviewed Original ResearchMeSH KeywordsAcetyl Coenzyme AAlcohol DrinkingAMP-Activated Protein KinasesAnimalsEthanolFatty AcidsFatty LiverGlucuronic AcidGlutamate-Cysteine LigaseGlutamatesGlutathioneHomeostasisLipogenesisLiverMaleMiceMice, Inbred C57BLMice, KnockoutOligonucleotide Array Sequence AnalysisOxidation-ReductionOxidative StressPentose Phosphate PathwayProtective AgentsTranscription, GeneticConceptsGlutamate-cysteine ligase modifier subunit geneProtein kinase pathwayAcetyl-CoA fluxMultiple cellular pathwaysAlcohol-induced steatosisCellular stressNucleotide biosynthesisLiver microarray analysisGlobal profilingSubunit geneCellular pathwaysMetabolic reprogrammingKinase pathwayMicroarray analysisMolecular mechanismsGSH poolCellular responsesMetabolic pathwaysLower GSHMolecular pathwaysMetabolic homeostasisAmino acidsDepletion of glutathioneCritical pathogenic eventGlucuronate pathway
2016
Catalase deletion promotes prediabetic phenotype in mice
Heit C, Marshall S, Singh S, Yu X, Charkoftaki G, Zhao H, Orlicky DJ, Fritz KS, Thompson DC, Vasiliou V. Catalase deletion promotes prediabetic phenotype in mice. Free Radical Biology And Medicine 2016, 103: 48-56. PMID: 27939935, PMCID: PMC5513671, DOI: 10.1016/j.freeradbiomed.2016.12.011.Peer-Reviewed Original ResearchConceptsCatalase-knockout (KO) micePancreatic morphological changesImpaired glucose toleranceDevelopment of diabetesSerum insulin levelsPre-diabetic phenotypePre-diabetic statusRNA-seq analysisSignal transduction moleculesMuscle lipid depositionSignal transduction mechanismsGlucose toleranceMetabolic syndromeInsulin levelsNormal chowHepatic triglyceridesObese phenotypeLiver morphologyHigh riskPrediabetic phenotypesKnockout miceLipid depositionBody weightTransduction moleculesMetabolic regulation
2010
Aldehyde Dehydrogenase 1B1: Molecular Cloning and Characterization of a Novel Mitochondrial Acetaldehyde-Metabolizing Enzyme
Stagos D, Chen Y, Brocker C, Donald E, Jackson BC, Orlicky DJ, Thompson DC, Vasiliou V. Aldehyde Dehydrogenase 1B1: Molecular Cloning and Characterization of a Novel Mitochondrial Acetaldehyde-Metabolizing Enzyme. Drug Metabolism And Disposition 2010, 38: 1679-1687. PMID: 20616185, PMCID: PMC2957164, DOI: 10.1124/dmd.110.034678.Peer-Reviewed Original ResearchMeSH KeywordsAcetaldehydeAldehyde DehydrogenaseAldehyde Dehydrogenase 1 FamilyAldehyde Dehydrogenase, MitochondrialAmino Acid SequenceAnimalsBaculoviridaeBlotting, WesternCell LineCloning, MolecularEthanolGenetic VectorsHumansImmunohistochemistryInsectaMaleMiceMice, Inbred C57BLMice, KnockoutMitochondriaMolecular Sequence DataNADOrgan SpecificityOxidation-ReductionPlasmidsRecombinant ProteinsReverse Transcriptase Polymerase Chain ReactionSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
2002
Genetic ablation of inducible nitric oxide synthase decreases mouse lung tumorigenesis.
Kisley L, Barrett B, Bauer A, Dwyer-Nield L, Barthel B, Meyer A, Thompson D, Malkinson A. Genetic ablation of inducible nitric oxide synthase decreases mouse lung tumorigenesis. Cancer Research 2002, 62: 6850-6. PMID: 12460898.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsButylated HydroxytolueneCarcinogensEndothelial Growth FactorsImmunohistochemistryIntercellular Signaling Peptides and ProteinsLungLung NeoplasmsLymphokinesMacrophages, AlveolarMaleMiceMice, KnockoutNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IIUrethaneVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsConceptsLung tumor multiplicityLung tumorigenesisLung tumorsMouse lung tumorigenesisINOS deficiencyINOS (-/-) miceNitric oxideLung inflammationTumor multiplicityLung cancer chemopreventive agentNitric oxide synthase contentInducible nitric oxide synthaseInducible nitric oxide synthase (iNOS) contentMurine lung tumorigenesisVascular endothelial growth factor (VEGF) expressionChronic lung inflammationRole of iNOSLung cancer patientsWhole lung extractsCOX-2 contentAlveolar macrophage infiltrationNitric oxide synthaseWild-type miceBronchiolar Clara cellsMouse lung tumorsCelecoxib reduces pulmonary inflammation but not lung tumorigenesis in mice
Kisley L, Barrett B, Dwyer-Nield L, Bauer A, Thompson D, Malkinson A. Celecoxib reduces pulmonary inflammation but not lung tumorigenesis in mice. Carcinogenesis 2002, 23: 1653-1660. PMID: 12376474, DOI: 10.1093/carcin/23.10.1653.Peer-Reviewed Original ResearchConceptsNon-steroidal anti-inflammatory drugsLung tumor formationPulmonary inflammationLung tumorigenesisLung tumorsMacrophage infiltrationPGE2 productionTumor formationMice fed control dietCOX-2-specific inhibitorsTwo-stage carcinogenesis protocolCelecoxib-treated miceLung cancer developmentAnti-inflammatory drugsProduction of eicosanoidsFed control dietCyclooxygenase-enzyme expressionDifferent organ systemsChronic administrationPGE2 levelsVascular leakageClinical trialsCarcinogenesis protocolMurine modelHigh risk
1998
Differential susceptibility of tracheal contraction to nonadrenergic noncholinergic relaxation.
Thompson D, Altiere R. Differential susceptibility of tracheal contraction to nonadrenergic noncholinergic relaxation. Journal Of Pharmacology And Experimental Therapeutics 1998, 284: 19-24. PMID: 9435155.Peer-Reviewed Original ResearchConceptsAirway smooth muscle contractionSmooth muscle contractionInduction of toneMuscle contractionNerve stimulationNitric oxide-dependent componentAirway smooth muscle toneNitric oxide synthase inhibitorOvalbumin-sensitized guinea pigsNerve-induced relaxationNonadrenergic noncholinergic relaxationElectrical field stimulationOxide synthase inhibitorSmooth muscle toneLevel of toneNoncholinergic relaxationTracheal contractionRelaxant responsesContractile actionAirway tissueNerve activationContractile agonistsMuscle toneField stimulationSynthase inhibitor
1997
Absence of tachykinin involvement in leukotriene D4 and antigen-induced contraction of guinea pig isolated bronchus.
Cibulsky S, Thompson D. Absence of tachykinin involvement in leukotriene D4 and antigen-induced contraction of guinea pig isolated bronchus. Journal Of Pharmacology And Experimental Therapeutics 1997, 283: 1102-9. PMID: 9399982.Peer-Reviewed Original ResearchConceptsD4-induced contractionAntigen-induced contractionGuinea-pig bronchiLeukotriene D4Endogenous tachykininsPig bronchiNeutral endopeptidaseLeukotriene D4-induced contractionMast cell-derived mediatorsAirway smooth muscle cellsConcentration of tachykininOvalbumin-induced contractionGuinea pig airwaysCell-derived mediatorsPresence of tetrodotoxinNeurokinin receptor antagonistsAngiotensin-converting enzymeSmooth muscle cellsTachykinin involvementTachykininergic responsesContractile actionPig airwaysReceptor antagonistTetrodotoxin treatmentTachykinins
1994
Characterization of acetylcholinesterase in rabbit intrapulmonary arteries
Altiere R, Travis D, Thompson D. Characterization of acetylcholinesterase in rabbit intrapulmonary arteries. American Journal Of Physiology 1994, 267: l745-l752. PMID: 7810679, DOI: 10.1152/ajplung.1994.267.6.l745.Peer-Reviewed Original ResearchConceptsAction of acetylcholinePulmonary blood vesselsCholinesterase activityLobar arteryIntrapulmonary arteriesBlood vesselsRabbit intrapulmonary arteriesACh-induced contractionConcentration-dependent inhibitionPulmonary arteryIntrapulmonary veinsPulmonary vasculatureTrachealis muscleMicroM neostigminePeripheral tissuesArteryEnzyme activityAcetylcholineCholinesterase inhibitionArterial enzymeSelective inhibitorMin/NeostigmineAortaCholinesterase enzymesPharmacological characterization of muscarinic receptors mediating acetylcholine-induced contraction and relaxation in rabbit intrapulmonary arteries.
Altiere R, Travis D, Roberts J, Thompson D. Pharmacological characterization of muscarinic receptors mediating acetylcholine-induced contraction and relaxation in rabbit intrapulmonary arteries. Journal Of Pharmacology And Experimental Therapeutics 1994, 270: 269-76. PMID: 8035325.Peer-Reviewed Original ResearchConceptsRelaxant responsesPA2 valuesContractile responsePulmonary arteryMuscarinic receptorsM1-selective antagonist pirenzepineEndothelium-dependent contractionsRabbit intrapulmonary arteriesEndothelium-dependent relaxationIsolated pulmonary arteriesM3-selective antagonistRabbit pulmonary arteryAcetylcholine-induced contractionsMuscarinic receptor subtypesN-methylpiperidine methiodideM2-selective antagonistSubtype-selective antagonistsNitric oxide synthaseInhibitor of cyclooxygenaseElevated toneAntagonist pirenzepineIntrapulmonary arteriesOxide synthaseReceptor subtypesPharmacological characterization
1992
Atypical presynaptic alpha-adrenoceptor modulation of neurally-mediated cholinergic responses in guinea-pig tracheal smooth muscle
Thompson D, Diamond L, Altiere R. Atypical presynaptic alpha-adrenoceptor modulation of neurally-mediated cholinergic responses in guinea-pig tracheal smooth muscle. Pulmonary Pharmacology & Therapeutics 1992, 5: 251-255. PMID: 1477481, DOI: 10.1016/0952-0600(92)90067-q.Peer-Reviewed Original ResearchConceptsGuinea pig tracheaGuinea pig tracheal smooth muscleInhibitory actionAlpha-adrenoceptor modulationCholinergic excitatory nervesCholinergic excitatory responsesIntermittent nerve stimulationAlpha-adrenoceptor agonistsPresynaptic inhibitory actionPresynaptic inhibitory effectAction of norepinephrineUse of norepinephrineNorepinephrine-induced inhibitionTracheal smooth musclePretreatment of tissuesConcentration-dependent inhibitionConcentration-dependent mannerCholinergic contractionsPresynaptic alphaCholinergic nervesExcitatory nervesNerve stimulationCholinergic responsesExcitatory responsesSmooth muscleModulation of cholinergic responses by Nω-nitro-l-arginine in rabbit intrapulmonary arteries
Altiere R, Thompson D. Modulation of cholinergic responses by Nω-nitro-l-arginine in rabbit intrapulmonary arteries. Pulmonary Pharmacology & Therapeutics 1992, 5: 149-151. PMID: 1611234, DOI: 10.1016/0952-0600(92)90034-e.Peer-Reviewed Original ResearchOmega-conotoxin-sensitive calcium channels modulate autonomic neurotransmission in guinea pig airways.
Altiere R, Diamond L, Thompson D. Omega-conotoxin-sensitive calcium channels modulate autonomic neurotransmission in guinea pig airways. Journal Of Pharmacology And Experimental Therapeutics 1992, 260: 98-103. PMID: 1309883.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsAtropineAutonomic Nervous SystemBronchiCalcium ChannelsCapsaicinCholineElectric StimulationEpinephrineGuanethidineGuinea PigsIndomethacinMaleMuscle ContractionNeurons, AfferentNeurons, EfferentOmega-Conotoxin GVIAPeptides, CyclicPropranololSynaptic TransmissionTachykininsTracheaConceptsSensitive calcium channelsCalcium channelsCholinergic excitatoryNeuronal N-type voltage-sensitive calcium channelsN-type voltage-sensitive calcium channelsNonadrenergic noncholinergic (NANC) inhibitory nervesNonadrenergic noncholinergic inhibitory responsesVoltage-sensitive calcium channelsNoncholinergic inhibitory nervesNoncholinergic inhibitory responsesGuinea pig airwaysGuinea-pig bronchiElectrical field stimulationGuinea pig tracheaMarine snail Conus geographusConcentration-response relationshipAirway nervesAutonomic neurotransmissionTachykininergic responsesExogenous acetylcholineInhibitory nervesContractile responseConotoxin GVIAPig airwaysExcitatory responses
1990
Enzymatic Modulation of Vasoactive Intestinal Peptide and Nonadrenergic Noncholinergic Inhibitory Responses in Guinea Pig Tracheae
Thompson D, Diamond L, Altiere R. Enzymatic Modulation of Vasoactive Intestinal Peptide and Nonadrenergic Noncholinergic Inhibitory Responses in Guinea Pig Tracheae. American Journal Of Respiratory And Critical Care Medicine 1990, 142: 1119-1123. PMID: 2240834, DOI: 10.1164/ajrccm/142.5.1119.Peer-Reviewed Original ResearchConceptsVasoactive intestinal peptideNANC inhibitory responsesGuinea pig tracheaConcentration-response curvesInhibitory responsesIntestinal peptideInhibitory systemPig tracheaNonadrenergic noncholinergic inhibitory responsesVIP concentration-response curveExogenous vasoactive intestinal peptideRelaxation responseExogenous VIP administrationNANC inhibitory neurotransmitterNANC nerve stimulationNANC relaxation responsesNoncholinergic inhibitory responsesElectrical field stimulationSoybean trypsin inhibitorAdrenergic inhibitoryTachykinin depletionCholinergic excitatoryVIP administrationAutonomic nervesNerve stimulationNonadrenergic bronchodilation induced by high concentrations of sulfur dioxide
Thompson D, Szarek J, Altiere R, Diamond L. Nonadrenergic bronchodilation induced by high concentrations of sulfur dioxide. Journal Of Applied Physiology 1990, 69: 1786-1791. PMID: 2272972, DOI: 10.1152/jappl.1990.69.5.1786.Peer-Reviewed Original ResearchConceptsBronchodilator responseNonadrenergic noncholinergicGanglionic blocking agent hexamethoniumAirway smooth muscle toneConcentration-dependent bronchodilationNANC inhibitory transmitterDynamic lung complianceCyclooxygenase inhibitor indomethacinSmooth muscle toneAgent hexamethoniumBronchoconstrictive stimuliNonadrenergic bronchodilationBronchomotor toneIntravenous atropineAutonomic reflexesInhibitory transmitterLung complianceLung resistanceContinuous infusionFeline airwaysMuscle toneCromolyn sodiumInhibitor indomethacinInhibitory systemBronchodilationPresynaptic alpha adrenoceptor modulation of neurally mediated cholinergic excitatory and nonadrenergic noncholinergic inhibitory responses in guinea pig trachea.
Thompson D, Diamond L, Altiere R. Presynaptic alpha adrenoceptor modulation of neurally mediated cholinergic excitatory and nonadrenergic noncholinergic inhibitory responses in guinea pig trachea. Journal Of Pharmacology And Experimental Therapeutics 1990, 254: 306-11. PMID: 2164097.Peer-Reviewed Original ResearchConceptsGuinea pig tracheaNANC inhibitory nervesAlpha-2 adrenoceptor agonistPig tracheaInhibitory nervesAdrenoceptor agonistsExcitatory nervesNerve stimulationAdrenoceptor antagonistInhibitory responsesAlpha-1 adrenoceptor agonistNonadrenergic noncholinergic inhibitory responsesPresynaptic alpha-2 adrenoceptorsAlpha-2 adrenoceptor antagonistAlpha-1 adrenoceptor antagonistAlpha-adrenoceptor modulationCholinergic excitatory nervesNANC inhibitory responsesNoncholinergic excitatory nervesNoncholinergic inhibitory responsesAction of clonidineAlpha-2 adrenoceptorsSympathetic nerve stimulationInhibitory nerve stimulationVasoactive intestinal peptideNICOTINIC AGONIST MODULATION OF FELINE BRONCHOMOTOR TONE
Thompson D, Altiere R, Diamond L. NICOTINIC AGONIST MODULATION OF FELINE BRONCHOMOTOR TONE. Clinical And Experimental Pharmacology And Physiology 1990, 17: 83-97. PMID: 2328544, DOI: 10.1111/j.1440-1681.1990.tb01291.x.Peer-Reviewed Original Research
1988
The effect of epithelium removal on non-adrenergic, non-cholinergic inhibitory responses in the isolated central airways of the cat and guinea pig
Thompson D, Wells J, Altiere R, Diamond L. The effect of epithelium removal on non-adrenergic, non-cholinergic inhibitory responses in the isolated central airways of the cat and guinea pig. European Journal Of Pharmacology 1988, 145: 231-237. PMID: 3350044, DOI: 10.1016/0014-2999(88)90237-3.Peer-Reviewed Original ResearchConceptsElectrical field stimulationEpithelium removalField stimulationHilar bronchusNon-cholinergic inhibitory responsesTissue sensitivityRelaxation responseNANC relaxation responsesGuinea pig tracheaBronchial sensitivityAirway preparationsContractile responseContractile actionContractile agonistsCentral airwaysPig tracheaAirway epitheliumInhibitory responsesPharmacological agonistsFeline tracheaGuinea pigsBronchusStimulationAgonistsTrachea