Quantification of Neural Ethanol and Acetaldehyde Using Headspace GC‐MS
Heit C, Eriksson P, Thompson DC, Charkoftaki G, Fritz KS, Vasiliou V. Quantification of Neural Ethanol and Acetaldehyde Using Headspace GC‐MS. Alcohol Clinical And Experimental Research 2016, 40: 1825-1831. PMID: 27501276, PMCID: PMC5008984, DOI: 10.1111/acer.13156.Peer-Reviewed Original ResearchConceptsAlcohol drinking behaviorEtOH metabolitesMurine brain tissueACh levelsAlcohol consumptionACh concentrationBrain tissueLiver tissueAChAlcohol addictionACh formationBrainDrinking behaviorLow micromolar rangeAlcohol researchTissue analysisTissueNanomolar rangeChromatography-mass spectrometry methodQuantitative assayMass spectrometry methodActive agentsMicromolar rangeSensitive gas chromatography-mass spectrometry methodHeme oxygenase 1 protects ethanol-administered liver tissue in Aldh2 knockout mice
Matsumoto A, Thompson D, Chen Y, Vasiliou V, Kawamoto T, Ichiba M. Heme oxygenase 1 protects ethanol-administered liver tissue in Aldh2 knockout mice. Alcohol 2016, 52: 49-54. PMID: 27139237, DOI: 10.1016/j.alcohol.2016.02.004.Peer-Reviewed Original ResearchConceptsAldh2 knockout miceStress-related proteinsOxidative stress-related proteinsAlanine transaminaseAnti-oxidative proteinsKnockout miceHealthy individualsHepatic tumor necrosis factor alphaLiver tissueProtective factorsTumor necrosis factor alphaSerum alanine transaminaseRecent epidemiological studiesNecrosis factor alphaWild-type miceHeme oxygenase-1Cytochrome P450 2E1ALDH2 proteinProteinAldehyde dehydrogenase 2 geneHepatic malondialdehydeMechanistic explanationInflammatory cytokinesEthanol administrationMechanistic hypotheses