The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
Görlach M, Burd C, Dreyfuss G. The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. Journal Of Biological Chemistry 1994, 269: 23074-23078. PMID: 8083209, DOI: 10.1016/s0021-9258(17)31621-6.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBinding SitesElectrophoresis, Polyacrylamide GelGene AmplificationHeterogeneous-Nuclear Ribonucleoprotein Group CHeterogeneous-Nuclear RibonucleoproteinsMolecular Sequence DataRibonucleoproteinsRNA, Heterogeneous NuclearRNA-Binding ProteinsSequence Homology, Amino AcidConceptsRNA-binding domainRNA-binding specificityHnRNP C1Selection/amplificationC proteinMinimal RNA-binding domainHeterogeneous nuclear ribonucleoprotein C proteinsHnRNP C proteinsRandom sequence RNARNA-binding propertiesDeterminant of RNAFilter binding assaysRNP motifGeneral RNASpecificity residesC1-binding siteSequence RNAMotif familyRNA ligandsU residuesContiguous stretchesRNAAmino acidsProteinVariable regionsThe mRNA Poly(A)-Binding Protein: Localization, Abundance, and RNA-Binding Specificity
Görlach M, Burd C, Dreyfuss G. The mRNA Poly(A)-Binding Protein: Localization, Abundance, and RNA-Binding Specificity. Experimental Cell Research 1994, 211: 400-407. PMID: 7908267, DOI: 10.1006/excr.1994.1104.Peer-Reviewed Original ResearchConceptsHuman PABPRNA binding specificityQuantitative immunoblotting experimentsMost eukaryotic mRNAsRNA-binding propertiesTranslation of mRNAsConfocal immunofluorescence microscopySelection/amplificationEukaryotic mRNAsOligonucleotide poolRNA sequencesRich sequencesCellular localizationBinding proteinHeLa cellsImmunofluorescence microscopyImmunoblotting experimentsLow turnover rateLow affinityPABPProteinAbundanceMRNAIntracellular concentrationTurnover rate