2014
A Conservative Amino Acid Mutation in the Master Regulator FleQ Renders Pseudomonas aeruginosa Aflagellate
Jain R, Kazmierczak BI. A Conservative Amino Acid Mutation in the Master Regulator FleQ Renders Pseudomonas aeruginosa Aflagellate. PLOS ONE 2014, 9: e97439. PMID: 24827992, PMCID: PMC4020848, DOI: 10.1371/journal.pone.0097439.Peer-Reviewed Original ResearchConceptsMurine pulmonary infectionIL-1 signalPresence of mutationsPulmonary infectionAcute infectionBacterial clearanceHost responseBacterial infectionsClinical strainsInfectionSystem expressionAmino acid changesAmino acid mutationsSingle amino acid changeStrain PA103ClearanceAmino acid substitutionsPseudomonas aeruginosa pathogenesisPathogen recognitionAcid changesPA103Negative correlationMotilityAcid mutationsConservative amino acid substitutionsCheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection
Czechowska K, McKeithen-Mead S, Al Moussawi K, Kazmierczak BI. Cheating by type 3 secretion system-negative Pseudomonas aeruginosa during pulmonary infection. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 7801-7806. PMID: 24821799, PMCID: PMC4040582, DOI: 10.1073/pnas.1400782111.Peer-Reviewed Original ResearchConceptsT3SS-inducing conditionsOpportunistic pathogen Pseudomonas aeruginosaType 3 secretion systemPathogen Pseudomonas aeruginosaWT P. aeruginosaPositive bacteriaFitness advantageDefective mutantsRelative fitnessSecretion systemBacterial virulenceSelective advantageMyD88 knockout miceNeutrophil-depleted animalsVitro growth rateMutantsInnate immune cellsPseudomonas aeruginosaP. aeruginosa infectionCompetition experimentsAcute pneumonia modelBacteriaFitnessPulmonary infectionImmunocompetent hosts
2012
Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations
Jain R, Behrens AJ, Kaever V, Kazmierczak BI. Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations. Journal Of Bacteriology 2012, 194: 4285-4294. PMID: 22685276, PMCID: PMC3416225, DOI: 10.1128/jb.00803-12.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Secretion SystemsCaulobacter crescentusCyclic GMPFimbriae, BacterialGene DeletionHydrolysisPseudomonas aeruginosaSuppression, GeneticConceptsType IV piliPilus assemblyDiguanylate cyclasesSurface organellesGGDEF/EAL domain proteinsType IV pilus assemblyPolar type IV piliEAL domain proteinsAssembly of piliGMP concentrationSuppressor screenCaulobacter crescentusDiguanylate cyclaseDomain proteinsSuppressor mutationsAdditional regulatorsSuppressor strainsRobust biofilmsDifferent environmental conditionsAssembly defectsNegative regulatorMicrocolony formationSurface piliFimXP. aeruginosa