2019
Integrin α5β1 regulates PP2A complex assembly through PDE4D in atherosclerosis
Yun S, Hu R, Schwaemmle ME, Scherer AN, Zhuang Z, Koleske AJ, Pallas DC, Schwartz MA. Integrin α5β1 regulates PP2A complex assembly through PDE4D in atherosclerosis. Journal Of Clinical Investigation 2019, 129: 4863-4874. PMID: 31408443, PMCID: PMC6819111, DOI: 10.1172/jci127692.Peer-Reviewed Original ResearchConceptsPP2A regulatory subunit B55αTranscription factor YAPActive PDEComplex assemblyAdapter rolePDE4D5B55αIntegrin α5EC phenotypeCell functionInflammatory signalingAthero-prone regionsActivationComplexesPP2AInflammatory activationWidespread consequencesDephosphorylationProteomicsVascular remodelingPlaque sizeAtherosclerotic plaque sizeSignalingYAPRegulates
2012
Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin–Arg–lysozyme complex
Liu W, MacGrath SM, Koleske AJ, Boggon TJ. Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin–Arg–lysozyme complex. Acta Crystallographica Section F: Structural Biology Communications 2012, 68: 154-158. PMID: 22297987, PMCID: PMC3274391, DOI: 10.1107/s1744309111056132.Peer-Reviewed Original ResearchConceptsCrystal structure determinationNonreceptor tyrosine kinaseArg nonreceptor tyrosine kinaseHeterotrimeric complexSH3 domainMacromolecular crystallographersCrystallography approachStructure determinationTyrosine kinaseCocrystal structureMolecular replacementTrace amountsLysozyme complexStructure solutionProteinCortactinComplexesCrystallizationCrystallographersCocrystalsKinaseMotifAutomatic model buildingSequenceArg
1995
The RNA polymerase II holoenzyme and its implications for gene regulation
Koleske A, Young R. The RNA polymerase II holoenzyme and its implications for gene regulation. Trends In Biochemical Sciences 1995, 20: 113-116. PMID: 7709429, DOI: 10.1016/s0968-0004(00)88977-x.Peer-Reviewed Original ResearchConceptsRNA polymerase II holoenzymeTranscription initiation apparatusGeneral transcription factorsRNA polymerase holoenzymeGene regulationPol IIPolymerase holoenzymeTranscriptional regulatorsTranscription factorsB proteinGene expressionLarge complexesSRB componentsHoloenzymeRegulationRecent evidenceComplexesSuppressorRegulatorProteinExpression
1994
An RNA polymerase II holoenzyme responsive to activators
Koleske A, Young R. An RNA polymerase II holoenzyme responsive to activators. Nature 1994, 368: 466-469. PMID: 8133894, DOI: 10.1038/368466a0.Peer-Reviewed Original ResearchConceptsRNA polymerase IIGeneral transcription factorsRNA polymerase II holoenzymePolymerase IITranscription factorsPromoter DNAMulti-component complexesGAL4-VP16Initiation factorsRegulatory proteinsHoloenzymeProteinDNAOrdered fashionCerevisiaeTranscriptionVivoPromoterActivatorAssemblyEquimolar amountsComplexes
1993
A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
Thompson C, Koleske A, Chao D, Young R. A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 1993, 73: 1361-1375. PMID: 8324825, DOI: 10.1016/0092-8674(93)90362-t.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBinding SitesDNA Mutational AnalysisDNA-Binding ProteinsFungal ProteinsGene Expression RegulationMediator ComplexMolecular Sequence DataMultienzyme ComplexesRecombinant ProteinsRNA Polymerase IISaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTATA-Box Binding ProteinTranscription FactorsTranscription, GeneticConceptsTATA-binding proteinRNA polymerase II carboxy-terminal domainCarboxy-terminal domainMultisubunit complexLarge multisubunit complexFunctional preinitiation complexRNA polymerase IIEfficient transcription initiationTranscription initiation complexSRB proteinsCTD proteinsExtragenic suppressorsCTD functionPolymerase IIPreinitiation complexTranscription initiationInitiation complexComplex bindsTruncation mutationsSRB2Srb5ProteinBiochemical evidenceComplexesSRB4