1997
The Regulation of Glycogen Synthase by Protein Phosphatase 1 in 3T3-L1 Adipocytes EVIDENCE FOR A POTENTIAL ROLE FOR DARPP-32 IN INSULIN ACTION*
Brady M, Nairn A, Saltiel A. The Regulation of Glycogen Synthase by Protein Phosphatase 1 in 3T3-L1 Adipocytes EVIDENCE FOR A POTENTIAL ROLE FOR DARPP-32 IN INSULIN ACTION*. Journal Of Biological Chemistry 1997, 272: 29698-29703. PMID: 9368038, DOI: 10.1074/jbc.272.47.29698.Peer-Reviewed Original ResearchConceptsProtein phosphatase 1PP1 activityGlycogen synthasePhosphatase 1DARPP-32Glycogen synthesisPrimary rat adipocytesPP1 proteinProtein DARPP-32Glycogen synthase activityKinetic lagSynthase activityGlycogen accumulationDifferentiationRat adipocytesAdipocytesFibroblast extractsAdipocyte cellsTotal glycogen synthase activitySynthaseInsulin actionPotential roleSpecific activityParticulate fractionInsulin pretreatment
1994
Role of elongation factor 2 in regulating peptide-chain elongation in the heart
Vary T, Nairn A, Lynch C. Role of elongation factor 2 in regulating peptide-chain elongation in the heart. American Journal Of Physiology 1994, 266: e628-e634. PMID: 7513958, DOI: 10.1152/ajpendo.1994.266.4.e628.Peer-Reviewed Original ResearchConceptsDiabetic ratsEF-2 contentFactor 2Protein synthesisInsulin therapyIncrease of RNADecreased translational efficiencyElongation factor 2RatsDiabetesCardiac muscleImpaired rateDecreased rateProgressive decreaseHeartInhibitionMolecular mechanismsRNA contentPeptide chain elongationH durationTherapyInsulinDecrease
1989
Insulin rapidly induces the biosynthesis of elongation factor 2
Levenson R, Nairn A, Blackshear P. Insulin rapidly induces the biosynthesis of elongation factor 2. Journal Of Biological Chemistry 1989, 264: 11904-11911. PMID: 2663845, DOI: 10.1016/s0021-9258(18)80152-1.Peer-Reviewed Original ResearchConceptsElongation factor 2EF-2Overall protein synthesisProtein synthesisProtein translation apparatusEukaryotic elongation factor 2Two-dimensional gel electrophoresisHuman insulin receptorNIH 3T3 cellsFactor 2Translation apparatusMature speciesMRNA translationActinomycin D. ThusRNA transcription inhibitorSuch proteinsIndividual proteinsInsulin inductionPreferential expressionPrecursor formInsulin receptorSerum deprivationProteinGel autoradiographsGel electrophoresis