2021
Rab27a-Dependent Paracrine Communication Controls Dendritic Spine Formation and Sensory Responses in the Barrel Cortex
Zhang L, Zhang X, Hsieh LS, Lin TV, Bordey A. Rab27a-Dependent Paracrine Communication Controls Dendritic Spine Formation and Sensory Responses in the Barrel Cortex. Cells 2021, 10: 622. PMID: 33799820, PMCID: PMC8000154, DOI: 10.3390/cells10030622.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedDendritic SpinesExcitatory Postsynaptic PotentialsExtracellular VesiclesFemaleGene Expression Regulation, DevelopmentalGestational AgeMiceParacrine CommunicationPregnancyPyramidal CellsRab27 GTP-Binding ProteinsSensory Receptor CellsSomatosensory CortexSynaptic TransmissionVibrissaeConceptsSmall extracellular vesiclesL4 neuronsParacrine communicationExcitatory synaptic transmissionRelease of sEVsDendritic spine formationCell-autonomous effectsRisk of autismL2/3 neuronsPyramidal neuronsLayer 2/3Somatosensory cortexBarrel cortexCortical neuronsSynaptic transmissionWhisker stimulationSomatosensory informationJuvenile miceSynaptic connectivitySynaptic integrationSpine formationBrain developmentNeuronsSensory stimulationSpine development
2016
Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP
Bartley CM, O’Keefe R, Blice-Baum A, Mihailescu MR, Gong X, Miyares L, Karaca E, Bordey A. Mammalian FMRP S499 Is Phosphorylated by CK2 and Promotes Secondary Phosphorylation of FMRP. ENeuro 2016, 3: eneuro.0092-16.2016. PMID: 27957526, PMCID: PMC5116651, DOI: 10.1523/eneuro.0092-16.2016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCasein Kinase IICells, CulturedCerebral CortexFragile X Mental Retardation ProteinHEK293 CellsHumansMass SpectrometryMiceNaphthyridinesPhenazinesPhosphorylationProtein BiosynthesisProtein Kinase InhibitorsReceptors, Metabotropic GlutamateRecombinant ProteinsTime FactorsConceptsTranslational repressionNearby residuesProtein phosphatase 2ACasein kinase IIMental retardation proteinFMRP lossPhosphatase 2AFragile X syndromePP2A pathwaySecondary phosphorylationProtein translationKinase IIGene transcriptsFMRPBrain transcriptsFunction mutationsPhosphorylationS499X syndromeTranscriptsRepressionResiduesRecent evidenceCK2Kinase
2014
BDNF Promotes Axon Branching of Retinal Ganglion Cells via miRNA-132 and p250GAP
Marler KJ, Suetterlin P, Dopplapudi A, Rubikaite A, Adnan J, Maiorano NA, Lowe AS, Thompson ID, Pathania M, Bordey A, Fulga T, Van Vactor DL, Hindges R, Drescher U. BDNF Promotes Axon Branching of Retinal Ganglion Cells via miRNA-132 and p250GAP. Journal Of Neuroscience 2014, 34: 969-979. PMID: 24431455, PMCID: PMC3891972, DOI: 10.1523/jneurosci.1910-13.2014.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorRetinal culturesMiRNA-132Neurotrophin brain-derived neurotrophic factorRetinal ganglion cell axonsAxon branchingGanglion cell axonsRetinal ganglion cellsMouse superior colliculusBDNF actionBDNF treatmentNeurotrophic factorRGC axonsTopographic targetingGanglion cellsCell axonsSuperior colliculusExpression of microRNAsTermination zonesP250GAPProtein levelsVertebrate visual systemAxonsUpregulationMap formation
2013
mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo
Hartman NW, Lin TV, Zhang L, Paquelet GE, Feliciano DM, Bordey A. mTORC1 Targets the Translational Repressor 4E-BP2, but Not S6 Kinase 1/2, to Regulate Neural Stem Cell Self-Renewal In Vivo. Cell Reports 2013, 5: 433-444. PMID: 24139800, DOI: 10.1016/j.celrep.2013.09.017.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCell Cycle ProteinsCell DifferentiationCells, CulturedEukaryotic Initiation FactorsMechanistic Target of Rapamycin Complex 1MiceMonomeric GTP-Binding ProteinsMultiprotein ComplexesNeural Stem CellsNeuropeptidesPhosphoproteinsPhosphorylationRas Homolog Enriched in Brain ProteinRibosomal Protein S6 Kinases, 90-kDaRNA InterferenceRNA, Small InterferingSirolimusTOR Serine-Threonine KinasesConceptsCap-dependent translationNeural stem cellsNSC differentiationStem Cell Self-RenewalTranslational repressor 4E-BP1P70 S6 kinase 1Neural Stem Cell Self-RenewalCell Self-RenewalRapamycin complex 1Neonatal neural stem cellsS6 kinase 1Downstream regulatory mechanismsLineage expansionSelf-RenewalRegulatory mechanismsKinase 1Kinase 1/2Constitutive activationMammalian targetCell growthStem cellsBrain sizeDifferentiationKnockdownNeuron production
2012
NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated Neural Progenitor Proliferation and Delays Dendrite Development
Young SZ, Taylor MM, Wu S, Ikeda-Matsuo Y, Kubera C, Bordey A. NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated Neural Progenitor Proliferation and Delays Dendrite Development. Journal Of Neuroscience 2012, 32: 13630-13638. PMID: 23015452, PMCID: PMC3478384, DOI: 10.1523/jneurosci.2864-12.2012.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsAnimals, NewbornCalciumCell CountCell DifferentiationCell ProliferationCells, CulturedCerebral VentriclesDendritesEgtazic AcidElectroporationFemaleGABA ModulatorsGABA-A Receptor AgonistsGreen Fluorescent ProteinsIn Vitro TechniquesKi-67 AntigenLuminescent ProteinsMaleMiceMuscimolNeural Stem CellsNeuronsOlfactory BulbPatch-Clamp TechniquesPentobarbitalReceptors, GABA-ARNA, Small InterferingSodium-Potassium-Chloride SymportersSolute Carrier Family 12, Member 2SOXB1 Transcription FactorsTransfectionConceptsNPC proliferationDecreased neuronal densityTotal dendritic lengthNeonatal subventricular zoneNeural stem cell proliferationNeural progenitor cell developmentNeural progenitor proliferationShort hairpin RNADendritic complexityDendritic lengthNeuronal densityNewborn neuronsDendritic arborizationNeuron densityDendritic developmentSubventricular zoneNeuron productionCalcium responseSynaptic integrationNKCC1 knockdownPentobarbital effectsAllosteric agonistDendritic treeProgenitor cell developmentCotransporter NKCC1
2010
MicroRNA miR‐137 Regulates Neuronal Maturation by Targeting Ubiquitin Ligase Mind Bomb‐1
Smrt RD, Szulwach KE, Pfeiffer RL, Li X, Guo W, Pathania M, Teng Z, Luo Y, Peng J, Bordey A, Jin P, Zhao X. MicroRNA miR‐137 Regulates Neuronal Maturation by Targeting Ubiquitin Ligase Mind Bomb‐1. Stem Cells 2010, 28: 1060-1070. PMID: 20506192, PMCID: PMC3140955, DOI: 10.1002/stem.431.Peer-Reviewed Original ResearchConceptsNeuronal maturationMiR-137Dendritic morphogenesisSpine developmentNovel miRNA-mediated mechanismCultured primary neuronsMiR-137 overexpressionConserved target sitesMiR-137 targetsBrain-enriched microRNAYoung neuronsMicroRNA miR-137Phenotypic maturationPrimary neuronsAxonal growthMiRNA-mediated mechanismMIB1Mind bomb-1NeuronsMessenger RNAMaturationOpposite effectOverexpressionMicroRNAsNeurodevelopment
2009
GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation
Dave KA, Bordey A. GABA increases Ca2+ in cerebellar granule cell precursors via depolarization: Implications for proliferation. IUBMB Life 2009, 61: 496-503. PMID: 19391160, PMCID: PMC2675662, DOI: 10.1002/iub.185.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidRole of GABAVertebrate central nervous systemComplement of receptorsCerebellar granule cell precursorsCell precursorsGranule cell precursorsDevelopmental processesMetabotropic glutamate receptorsCentral nervous systemCell proliferationAdult mammalsNovel targetAmino acid glutamatePharmacological regulationCurrent knowledgeGlutamate receptorsNeural cancersNervous systemCerebellar tumorsCalcium increaseReceptorsProliferationMammalsNovel data
2008
Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone
Platel J, Heintz T, Young S, Gordon V, Bordey A. Tonic activation of GLUK5 kainate receptors decreases neuroblast migration in whole‐mounts of the subventricular zone. The Journal Of Physiology 2008, 586: 3783-3793. PMID: 18565997, PMCID: PMC2538932, DOI: 10.1113/jphysiol.2008.155879.Peer-Reviewed Original ResearchConceptsKainate receptorsSVZ neuroblastsLateral ventricleSubventricular zoneNeuroblast migrationAcute slicesMetabotropic glutamate receptor subtype 5AMPA/kainate receptorsMGluR5 antagonist MPEPPermeable kainate receptorsReceptor antagonist bicucullineActivation of mGluR5Postnatal day 20Postnatal subventricular zoneDCX promoterAntagonist MPEPWhole-mount preparationsAntagonist bicucullineMGluR5 activationCell aspiratesReceptor antagonistMouse slicesTonic activationSubtype 5Immunopositive cells
1999
Differential Inhibition of Glial K+ Currents by 4-AP
Bordey A, Sontheimer H. Differential Inhibition of Glial K+ Currents by 4-AP. Journal Of Neurophysiology 1999, 82: 3476-3487. PMID: 10601476, DOI: 10.1152/jn.1999.82.6.3476.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAction PotentialsAnimalsAstrocytesCells, CulturedDelayed Rectifier Potassium ChannelsElectrophysiologyLarge-Conductance Calcium-Activated Potassium ChannelsNeurogliaPatch-Clamp TechniquesPotassium Channel BlockersPotassium ChannelsPotassium Channels, Calcium-ActivatedPotassium Channels, Inwardly RectifyingPotassium Channels, Voltage-GatedRatsRats, Sprague-DawleySpinal CordTetraethylammoniumConceptsAcute spinal cord slicesSpinal cord slicesConcentration-dependent blockPotassium channel typesType currentsDose-dependent inhibitorNeuronal action potentialsCord slicesSpinal cordChannel blockersEpileptiform dischargesBrain slicesAction potentialsHomeostatic functionsA-currentAstrocytesDifferential inhibitionRepolarizationMembrane potentialChannel typesSlicesBlockersGlialCord
1998
Passive Glial Cells, Fact or Artifact?
Bordey A, Sontheimer H. Passive Glial Cells, Fact or Artifact? The Journal Of Membrane Biology 1998, 166: 213-222. PMID: 9843595, DOI: 10.1007/s002329900463.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsVoltage-activated currentsPassive astrocytesVoltage-dependent outwardCell accessAcute tissue slicesBath Ca2Glial cellsRat hippocampusSlice recordingsCultured astrocytesAstrocytesPipette solutionEffect of Ca2Tissue slicesMembrane capacitanceSuch cellsSubpopulationsCellsTransient maskingConcentration of Ca2Ca2SuperfusionHippocampusDose