2012
Emergence of bimodal cell population responses from the interplay between analog single-cell signaling and protein expression noise
Birtwistle MR, Rauch J, Kiyatkin A, Aksamitiene E, Dobrzyński M, Hoek JB, Kolch W, Ogunnaike BA, Kholodenko BN. Emergence of bimodal cell population responses from the interplay between analog single-cell signaling and protein expression noise. BMC Systems Biology 2012, 6: 109. PMID: 22920937, PMCID: PMC3484110, DOI: 10.1186/1752-0509-6-109.Peer-Reviewed Original ResearchConceptsProtein expression noiseSingle-cell signalingExtracellular signal-regulated kinaseExpression noiseCell variabilityEpidermal growth factor stimulationProtein abundance variationCell fate decisionsPopulation responsesGrowth factor stimulationSingle cellsSignal-regulated kinaseCell population levelProtein expressionSingle-cell levelERK pathway activationFate decisionsPopulation levelFactor stimulationCell signalingCell population responseERK responseBiological outcomesPathway activationCell level
2010
Dynamic cross‐talk between PI3‐kinase/Akt and Ras/ERK pathways in EGF receptor signaling that can affect drug sensitivity in tumor cells
Aksamitiene E, Kiyatkin A, Hoek J. Dynamic cross‐talk between PI3‐kinase/Akt and Ras/ERK pathways in EGF receptor signaling that can affect drug sensitivity in tumor cells. The FASEB Journal 2010, 24: 715.2-715.2. DOI: 10.1096/fasebj.24.1_supplement.715.2.Peer-Reviewed Original ResearchPI3KRas/ERK pathwayPI3-kinase/AktPI3K activityEGF dose-dependent mannerNegative feedback loopAdaptor proteinMitogenic signalingU0126 inhibitorIndependent parallel pathwaysERK activityEGF receptorERK pathwayCell survivalK activityReciprocal crosstalkERK responseRecruitment mechanismsPhosphorylated ERKImmunoblot analysisEGF dosesERKAkt expressionSubcellular fractionsCancer cells