Differential regulation of glucocorticoid receptor transcriptional activation via AF‐1‐associated proteins
Hittelman A, Burakov D, Iñiguez‐Lluhí J, Freedman L, Garabedian M. Differential regulation of glucocorticoid receptor transcriptional activation via AF‐1‐associated proteins. The EMBO Journal 1999, 18: 5380-5388. PMID: 10508170, PMCID: PMC1171607, DOI: 10.1093/emboj/18.19.5380.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineDNA-Binding ProteinsEndosomal Sorting Complexes Required for TransportGene Expression RegulationGlucocorticoidsHeLa CellsHumansNuclear ProteinsProtein BindingReceptors, GlucocorticoidTrans-ActivatorsTranscription FactorsTranscriptional ActivationTwo-Hybrid System TechniquesConceptsTranscriptional enhancementAF-1Regulatory factorsGlucocorticoid receptor transcriptional activationTumor susceptibility gene 101Two-hybrid approachTranscriptional regulatory factorsSusceptibility gene 101Activation functions AF-1AF-2Negative regulatory factorHormone-activated glucocorticoid receptorHormone-dependent mannerModified yeastTranscriptional activationMammalian cellsDRIP complexTarget genesProtein 150Differential regulationGlucocorticoid receptorGR transactivationTransactivationYeastGR ligandsDistinct Glucocorticoid Receptor Transcriptional Regulatory Surfaces Mediate the Cytotoxic and Cytostatic Effects of Glucocorticoids
Rogatsky I, Hittelman A, Pearce D, Garabedian M. Distinct Glucocorticoid Receptor Transcriptional Regulatory Surfaces Mediate the Cytotoxic and Cytostatic Effects of Glucocorticoids. Molecular And Cellular Biology 1999, 19: 5036-5049. PMID: 10373553, PMCID: PMC84339, DOI: 10.1128/mcb.19.7.5036.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsApoptosisCell CycleCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase Inhibitor p27CyclinsCytotoxicity, ImmunologicDimerizationGlucocorticoidsHumansMicrotubule-Associated ProteinsMifepristoneMutagenesisProto-Oncogene Proteins c-bcl-2Receptors, GlucocorticoidTranscriptional ActivationTumor Cells, CulturedTumor Suppressor ProteinsConceptsTranscriptional repression functionRepression of Bcl2Saos2 cellsTranscriptional regulatory mechanismsAF-1Transcriptional activation functionTransactivation-deficient mutantAF-2Cyclin-dependent kinase inhibitorRepression functionTranscriptional activatorGlucocorticoid receptorRegulatory surfaceTarget genesCell contextReceptor dimerizationRegulatory mechanismsCytostatic actionHuman osteosarcoma cellsVariety of cellsGR mutantsCytostatic effectMolecular mechanismsCytotoxic effectsU20S cells