Vincent Graziano
Postdoctoral AssociateAbout
Research
Publications
Featured Publications
RNA N-glycosylation enables immune evasion and homeostatic efferocytosis
Graziano V, Porat J, Ah Kioon M, Mejdrová I, Matz A, Lebedenko C, Chai P, Pluvinage J, Ricci-Azevedo R, Harrison A, Wright S, Wang X, Strine M, Wang P, Wilson M, Vanaja S, Zhou B, Barrat F, Carell T, Flynn R, Rathinam V. RNA N-glycosylation enables immune evasion and homeostatic efferocytosis. Nature 2025, 645: 784-792. PMID: 40770106, PMCID: PMC12845878, DOI: 10.1038/s41586-025-09310-6.Peer-Reviewed Original ResearchConceptsnon-inflammatory clearanceN-glycanssmall RNAsinnate immune sensingapoptotic cellsendogenous small RNAsfunctional significanceproduction of type I interferonsde-N-glycosylationinnate immune activationendosomal networkinnate immune responseclearance of dead cellsN-glycosylationRNA sensorstype I interferonToll-like receptorsRNAglycoRNAcell surfacegenetic deletionI interferondead cellsacp3Uautoinflammatory responses
2024
Intestinal tuft cell immune privilege enables norovirus persistence
Strine M, Fagerberg E, Darcy P, Barrón G, Filler R, Alfajaro M, D'Angelo-Gavrish N, Wang F, Graziano V, Menasché B, Damo M, Wang Y, Howitt M, Lee S, Joshi N, Mucida D, Wilen C. Intestinal tuft cell immune privilege enables norovirus persistence. Science Immunology 2024, 9: eadi7038-eadi7038. PMID: 38517952, PMCID: PMC11555782, DOI: 10.1126/sciimmunol.adi7038.Peer-Reviewed Original ResearchConceptsCD8<sup>+</sup> T cellsintestinal tuft cellsT cellsTufted Cellsviral persistencesite of viral persistencechemosensory epithelial cellsnormal antigen presentationimmune-privileged nicheintestinal stem cellsmemory phenotypeimmune privilegeimmune escapereporter miceantigen presentationchronic infectioncytotoxic capacityepithelial cellsnorovirus infectionstem cellscell interactionsinfectioncell survivalenteric microbescells
2023
The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression
Wei J, Alfajaro M, Cai W, Graziano V, Strine M, Filler R, Biering S, Sarnik S, Patel S, Menasche B, Compton S, Konermann S, Hsu P, Orchard R, Yan Q, Wilen C. The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression. PLOS Pathogens 2023, 19: e1011351. PMID: 37410700, PMCID: PMC10325096, DOI: 10.1371/journal.ppat.1011351.Peer-Reviewed Original ResearchConceptsmouse hepatitis virusreceptor expressiontherapeutic targetMERS-CoVmajor SARS-CoV-2 variantsprimary human airwaySARS-CoV-2 variantsnovel therapeutic targetviral receptor expressionSARS-CoV-2histone methyltransferase KMT2Dintestinal epithelial cellsCoronavirus Susceptibilitydiverse coronaviruseshistone demethylase KDM6ADPP4 expressioncoronavirus receptorshost determinantshepatitis virushuman airwaysSARS-CoVsmall molecule inhibitionviral entrypotential drug targetsviral receptors
2022
Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity
Strine M, Alfajaro M, Graziano V, Song J, Hsieh L, Hill R, Guo J, VanDussen K, Orchard R, Baldridge M, Lee S, Wilen C. Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity. Cell Reports 2022, 41: 111593. PMID: 36351394, PMCID: PMC9662704, DOI: 10.1016/j.celrep.2022.111593.Peer-Reviewed Original Research
2021
UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling
Ketkar H, Harrison A, Graziano V, Geng T, Yang L, Vella A, Wang P. UBX Domain Protein 6 Positively Regulates JAK-STAT1/2 Signaling. The Journal Of Immunology 2021, 206: 2682-2691. PMID: 34021047, PMCID: PMC8164993, DOI: 10.4049/jimmunol.1901337.Peer-Reviewed Original ResearchConceptstyrosine kinase 2domain-containing proteinsexpression of hundredscomplex cellular regulationRNA viral replicationJAK/STATcellular regulationtype I/III IFNskinase 2JAK/type I/III IFNprotein 6IFN expressiongenesviral infectionsignalingexpressionviral replicationexpression of IFNcellstype IproteinSTAToverexpressiondeletionNorovirus evolution in immunodeficient mice reveals potentiated pathogenicity via a single nucleotide change in the viral capsid
Walker FC, Hassan E, Peterson ST, Rodgers R, Schriefer LA, Thompson CE, Li Y, Kalugotla G, Blum-Johnston C, Lawrence D, McCune BT, Graziano VR, Lushniak L, Lee S, Roth AN, Karst SM, Nice TJ, Miner JJ, Wilen CB, Baldridge MT. Norovirus evolution in immunodeficient mice reveals potentiated pathogenicity via a single nucleotide change in the viral capsid. PLOS Pathogens 2021, 17: e1009402. PMID: 33705489, PMCID: PMC7987144, DOI: 10.1371/journal.ppat.1009402.Peer-Reviewed Original ResearchConceptsnucleotide changessingle nucleotide changeviral capsidamino acid changesevolutionary potentialIFN-competent hostsintestinal myeloid cellsselective pressuresingle nucleotidekey controllernorovirus evolutionacid changeslethal pathogenvirus growthenhanced virulencemice revealsIFN responsehigh replicationenhanced recruitmentmyeloid cellsextraintestinal disseminationintestinal replicationreplicationpathogenicitycapsidCD300lf Conditional Knockout Mouse Reveals Strain-Specific Cellular Tropism of Murine Norovirus
Graziano VR, Alfajaro MM, Schmitz CO, Filler RB, Strine MS, Wei J, Hsieh LL, Baldridge MT, Nice TJ, Lee S, Orchard RC, Wilen CB. CD300lf Conditional Knockout Mouse Reveals Strain-Specific Cellular Tropism of Murine Norovirus. Journal Of Virology 2021, 95: 10.1128/jvi.01652-20. PMID: 33177207, PMCID: PMC7925115, DOI: 10.1128/jvi.01652-20.Peer-Reviewed Original ResearchConceptsconditional knockout miceintestinal epithelial cellscell tropismknockout micetuft cellsdendritic cellsmyelomonocytic cellsB cellscellular tropismmurine norovirusepithelial cellsviral RNA levelsinnate immune responsecause of gastroenteritisMNoV infectioncell typesviral loadgastrointestinal infectionsreceptor expressionimmunocompetent humansimmune responsecell type-specific rolesmouse modelintestinal tissueMNoV
2020
Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection
Wei J, Alfajaro MM, DeWeirdt PC, Hanna RE, Lu-Culligan WJ, Cai WL, Strine MS, Zhang SM, Graziano VR, Schmitz CO, Chen JS, Mankowski MC, Filler RB, Ravindra NG, Gasque V, de Miguel FJ, Patil A, Chen H, Oguntuyo KY, Abriola L, Surovtseva YV, Orchard RC, Lee B, Lindenbach BD, Politi K, van Dijk D, Kadoch C, Simon MD, Yan Q, Doench JG, Wilen CB. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell 2020, 184: 76-91.e13. PMID: 33147444, PMCID: PMC7574718, DOI: 10.1016/j.cell.2020.10.028.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin-Converting Enzyme 2AnimalsCell LineChlorocebus aethiopsClustered Regularly Interspaced Short Palindromic RepeatsCoronavirusCoronavirus InfectionsCOVID-19Gene Knockout TechniquesGene Regulatory NetworksGenome-Wide Association StudyHEK293 CellsHMGB1 ProteinHost-Pathogen InteractionsHumansSARS-CoV-2Vero CellsVirus InternalizationConceptsSARS-CoV-2 infectionSARS-CoV-2vesicular stomatitis virusgenome-wide CRISPR screenSWI/SNF chromatinSARS-CoV-2 host factorsacute respiratory syndrome coronavirus 2 infectionsevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectiontherapeutic targethost factorscoronavirus disease 2019 (COVID-19) pathogenesissyndrome coronavirus 2 infectionCRISPR screenshost genesgene productsMiddle East respiratory syndrome CoVcoronavirus 2 infectiongenetic hitshuman cellsSARS-CoV-2 spikenovel therapeutic targetpotential therapeutic targetVero E6 cellsSARS-CoV-1small molecule antagonists