2023
Targeted and selective knockout of the TLQP-21 neuropeptide unmasks its unique role in energy homeostasis
Sahu B, Razzoli M, McGonigle S, Pallais J, Nguyen M, Sadahiro M, Jiang C, Lin W, Kelley K, Rodriguez P, Mansk R, Cero C, Caviola G, Palanza P, Rao L, Beetch M, Alejandro E, Sham Y, Frontini A, Salton S, Bartolomucci A. Targeted and selective knockout of the TLQP-21 neuropeptide unmasks its unique role in energy homeostasis. Molecular Metabolism 2023, 76: 101781. PMID: 37482186, PMCID: PMC10400922, DOI: 10.1016/j.molmet.2023.101781.Peer-Reviewed Original ResearchConceptsGenetic engineering approachesUnique metabolic phenotypeMass spectrometry identificationPrecursor geneGel digestionGenetic lossTLQP-21Cleavage siteC-terminal arginineGenesMutant sequencesSelective knockoutEssential roleBiological constraintsMetabolic phenotypeMouse linesEnergy homeostasisComposite phenotypeMutant miceValuable resourceVGF gene
2020
Multiscale causal networks identify VGF as a key regulator of Alzheimer’s disease
Beckmann ND, Lin WJ, Wang M, Cohain AT, Charney AW, Wang P, Ma W, Wang YC, Jiang C, Audrain M, Comella PH, Fakira AK, Hariharan SP, Belbin GM, Girdhar K, Levey AI, Seyfried NT, Dammer EB, Duong D, Lah JJ, Haure-Mirande JV, Shackleton B, Fanutza T, Blitzer R, Kenny E, Zhu J, Haroutunian V, Katsel P, Gandy S, Tu Z, Ehrlich ME, Zhang B, Salton SR, Schadt EE. Multiscale causal networks identify VGF as a key regulator of Alzheimer’s disease. Nature Communications 2020, 11: 3942. PMID: 32770063, PMCID: PMC7414858, DOI: 10.1038/s41467-020-17405-z.Peer-Reviewed Original ResearchMeSH KeywordsAgedAged, 80 and overAlzheimer DiseaseAmyloid beta-PeptidesAnimalsBrainDatasets as TopicDisease Models, AnimalFemaleGene Expression ProfilingGene Regulatory NetworksGenome-Wide Association StudyHumansMaleMiceMice, TransgenicNerve Growth FactorsProtein Interaction MappingProtein Interaction MapsProteomics