Madison Strine, PhD
Postdoctoral AssociateAbout
Research
Publications
Featured Publications
Intestinal tuft cell immune privilege enables norovirus persistence
Strine M, Fagerberg E, Darcy P, Barrón G, Filler R, Alfajaro M, D'Angelo-Gavrish N, Wang F, Graziano V, Menasché B, Damo M, Wang Y, Howitt M, Lee S, Joshi N, Mucida D, Wilen C. Intestinal tuft cell immune privilege enables norovirus persistence. Science Immunology 2024, 9: eadi7038. PMID: 38517952, PMCID: PMC11555782, DOI: 10.1126/sciimmunol.adi7038.Peer-Reviewed Original ResearchConceptsCD8+ T cellsIntestinal tuft cellsT cellsTufted CellsViral persistenceSite of viral persistenceChemosensory epithelial cellsNormal antigen presentationImmune-privileged nicheIntestinal stem cellsMemory phenotypeImmune privilegeImmune escapeReporter miceAntigen presentationChronic infectionCytotoxic capacityEpithelial cellsNorovirus infectionStem cellsCell interactionsInfectionCell survivalEnteric microbesCellsDYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner
Strine M, Cai W, Wei J, Alfajaro M, Filler R, Biering S, Sarnik S, Chow R, Patil A, Cervantes K, Collings C, DeWeirdt P, Hanna R, Schofield K, Hulme C, Konermann S, Doench J, Hsu P, Kadoch C, Yan Q, Wilen C. DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. PLOS Biology 2023, 21: e3002097. PMID: 37310920, PMCID: PMC10263356, DOI: 10.1371/journal.pbio.3002097.Peer-Reviewed Original ResearchConceptsGenome-wide CRISPR/Cas9 screenCRISPR/Cas9 screenPathogenic human coronavirusesKinase-independent mannerRegulated kinase 1AProviral host factorNovel drug targetsMultiple cell typesDNA accessibilityHost factorsKinase functionHuman coronavirusesHost genesDistal enhancerNovel regulatorCas9 screenKinase 1AGene expressionNeuronal developmentDYRK1ADrug targetsDiverse coronavirusesProviral activityCell typesSevere acute respiratory syndrome coronavirus 2Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity
Strine M, Alfajaro M, Graziano V, Song J, Hsieh L, Hill R, Guo J, VanDussen K, Orchard R, Baldridge M, Lee S, Wilen C. Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity. Cell Reports 2022, 41: 111593. PMID: 36351394, PMCID: PMC9662704, DOI: 10.1016/j.celrep.2022.111593.Peer-Reviewed Original ResearchTuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces
Strine MS, Wilen CB. Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces. PLOS Pathogens 2022, 18: e1010318. PMID: 35271673, PMCID: PMC8912186, DOI: 10.1371/journal.ppat.1010318.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsInterkingdom interactionsTuft cellsCell biologyImmune responseMicrobial activationMicrobial sensingCell abundanceMucosal barrier surfacesAntiviral adaptive immune responsesType 2 immune responsesCell heterogeneityExquisite specificityMucosal barrier integrityAdaptive immune responsesMurine norovirusHuman healthKey orchestratorsMicrobial infectionsPathogenic bacteriaBroad intraFlavivirus replicationKey mediatorContext of coinfectionTissue repairImmune evasion
2025
Compartmentalized persistence of unconventional intestine-resident CD8⍺⍺ IEL throughout life 3477
Ruscher R, Miles K, Smith M, Strine M, Guter J, Kupz A, Hamilton S, Konnikova L, Jameson S. Compartmentalized persistence of unconventional intestine-resident CD8⍺⍺ IEL throughout life 3477. The Journal Of Immunology 2025, 214 DOI: 10.1093/jimmun/vkaf283.1277.Peer-Reviewed Original ResearchIntestinal intraepithelial lymphocytesSurface markersHuman gut tissueHuman immune responseParabiosis studiesT cellsIntraepithelial lymphocytesMouse modelImmune responseVaccination strategiesMouse coloniesSPF miceAged animalsGut homeostasisMiceOlder ageEarly lifeSmall intestineFate mappingHuman intestineSingle-cell analysisHuman fetal small intestinal T cells induce epithelial growth and secretory lineage differentiation 2280
Strine M, Llivichuzhca-Loja D, Wang W, Gu W, St Denis K, Gonzalez Santiago E, Zeve D, Breault D, Tseng G, Konnikova L. Human fetal small intestinal T cells induce epithelial growth and secretory lineage differentiation 2280. The Journal Of Immunology 2025, 214 DOI: 10.1093/jimmun/vkaf283.215.Peer-Reviewed Original ResearchMucosal T cellsT cellsSecond-trimesterEpithelial growthSecond-trimester of pregnancyFetal T cellsFetal immune systemAdult T cellsTrimester of pregnancyFetal small intestineSmall intestineHomeostatic functionsIntestinal T cellsCo-CultureEpithelial cell fateOrganoid growthFetal rejectionNecrotizing enterocolitisCo-culture systemAdult donorsPostnatal lifeSingle-cell RNA sequencingDonor matchingImmune systemIntestinal pathologyRNA N-glycosylation enables immune evasion and homeostatic efferocytosis
Graziano V, Porat J, Ah Kioon M, Mejdrová I, Matz A, Lebedenko C, Chai P, Pluvinage J, Ricci-Azevedo R, Harrison A, Wright S, Wang X, Strine M, Wang P, Wilson M, Vanaja S, Zhou B, Barrat F, Carell T, Flynn R, Rathinam V. RNA N-glycosylation enables immune evasion and homeostatic efferocytosis. Nature 2025, 645: 784-792. PMID: 40770106, DOI: 10.1038/s41586-025-09310-6.Peer-Reviewed Original ResearchConceptsNon-inflammatory clearanceN-glycansSmall RNAsInnate immune sensingApoptotic cellsEndogenous small RNAsFunctional significanceProduction of type I interferonsDe-N-glycosylationInnate immune activationEndosomal networkInnate immune responseClearance of dead cellsN-glycosylationRNA sensorsType I interferonToll-like receptorsRNAGlycoRNACell surfaceGenetic deletionI interferonDead cellsAcp3UAutoinflammatory responsesBugged before birth?: How maternal microbes reprogram offspring immunity
Strine M, Konnikova L. Bugged before birth?: How maternal microbes reprogram offspring immunity. Cell Host & Microbe 2025, 33: 1036-1038. PMID: 40639328, DOI: 10.1016/j.chom.2025.06.003.Peer-Reviewed Original ResearchExploring mucosal immune development and function in utero
Strine M, Vahkal B, St Denis K, Konnikova L. Exploring mucosal immune development and function in utero. Trends In Immunology 2025, 46: 502-504. PMID: 40518358, PMCID: PMC12255524, DOI: 10.1016/j.it.2025.05.004.Peer-Reviewed Original ResearchNorovirus co-opts NINJ1 for selective protein secretion
Song J, Zhang L, Moon S, Fang A, Wang G, Gheshm N, Loeb S, Cao P, Wallace J, Alfajaro M, Strine M, Beatty W, Jamieson A, Orchard R, Robinson B, Nice T, Wilen C, Orvedahl A, Reese T, Lee S. Norovirus co-opts NINJ1 for selective protein secretion. Science Advances 2025, 11: eadu7985. PMID: 40020060, PMCID: PMC11870086, DOI: 10.1126/sciadv.adu7985.Peer-Reviewed Original ResearchConceptsPlasma membrane ruptureDamage-associated molecular patternsNS1 secretionNinjurin-1Programmed Cell DeathAmino acid residuesViral replication sitesViral protein NS1CRISPR screensIntracellular viral proteinsMutagenesis studiesMembrane ruptureProtein NS1Unconventional pathwayCaspase-3Protein secretionViral proteinsReplication sitesCell deathMolecular patternsGenetic ablationNS1Pharmaceutical inhibitionDAMP releaseProtein