Isabelle Leo
Postdoctoral FellowAbout
Research
Publications
2025
Comparative evaluation of Olink Explore 3072 and mass spectrometry with peptide fractionation for plasma proteomics
Sissala N, Babačić H, Leo I, Cao X, Forshed J, Eriksson L, Lehtiö J, Fredolini C, Åberg M, Pernemalm M. Comparative evaluation of Olink Explore 3072 and mass spectrometry with peptide fractionation for plasma proteomics. Communications Chemistry 2025, 8: 327. PMID: 41188494, PMCID: PMC12586489, DOI: 10.1038/s42004-025-01753-2.Peer-Reviewed Original ResearchUsing TCR-CAR dual signaling for precise cancer targeting
Shahbazy M, Leo I, Faridi P, Caron E. Using TCR-CAR dual signaling for precise cancer targeting. Trends In Immunology 2025, 46: 435-437. PMID: 40368698, DOI: 10.1016/j.it.2025.04.007.Commentaries, Editorials and LettersMBPeace treaty: How myelin self-peptides broker CNS tolerance.
Leo I, Caron E. MBPeace treaty: How myelin self-peptides broker CNS tolerance. Science Immunology 2025, 10: eadx0208. PMID: 40053606, DOI: 10.1126/sciimmunol.adx0208.Commentaries, Editorials and LettersFunctional proteoform group deconvolution reveals a broader spectrum of ibrutinib off-targets
Leo I, Kunold E, Audrey A, Tampere M, Eirich J, Lehtiö J, Jafari R. Functional proteoform group deconvolution reveals a broader spectrum of ibrutinib off-targets. Nature Communications 2025, 16: 1948. PMID: 40000607, PMCID: PMC11862126, DOI: 10.1038/s41467-024-54654-8.Peer-Reviewed Original ResearchConceptsthermal proteome profilingproteome-wide profilingGolgi traffickingendosomal traffickingprotein variationcellular processestarget landscapeproteomic profilingdrug targetsfunctional impactoff-targetsprecision medicinetraffickingbiological effectsGolgiex vivo responseglycosylationproteoformstarget
2024
Molecular Profiling Defines Three Subtypes of Synovial Sarcoma
Chen Y, Su Y, Cao X, Siavelis I, Leo I, Zeng J, Tsagkozis P, Hesla A, Papakonstantinou A, Liu X, Huang W, Zhao B, Haglund C, Ehnman M, Johansson H, Lin Y, Lehtiö J, Zhang Y, Larsson O, Li X, de Flon F. Molecular Profiling Defines Three Subtypes of Synovial Sarcoma. Advanced Science 2024, 11: 2404510. PMID: 39257029, PMCID: PMC11892499, DOI: 10.1002/advs.202404510.Peer-Reviewed Original ResearchConceptssynovial sarcomapoor prognosisresponse to neoadjuvant therapyhigh-risk tumorsproportion of patientscheckpoint inhibitionneoadjuvant therapyrisk tumorsbiphasic tumorhistological subtypeschromosomal abnormalitiesimmune suppressionepithelial differentiationsingle-cell RNA sequencingsubtype IIIimmune detectionrisk factorssubtype IITargeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia
Bwanika H, Leo I, Struyf N, Talanti A, Aswad L, Konnur A, Björklund A, Heyman M, Rassidakis G, Erkers T, Seashore-Ludlow B, Jafari R, Pokrovskaja Tamm K. Targeting autophagy as a therapeutic strategy in pediatric acute lymphoblastic leukemia. Scientific Reports 2024, 14: 4000. PMID: 38369625, PMCID: PMC10874937, DOI: 10.1038/s41598-024-54400-6.Peer-Reviewed Original ResearchConceptsacute lymphoblastic leukemiacell linespediatric acute lymphoblastic leukemiaanalysis of proteomesprimary samplesRNA-seqgenetic rearrangementsALL cell linespathway enrichmentanti-cancer therapylymphoblastic leukemiainhibition of autophagygenetic subgroupslysosomal inhibitorstherapeutic strategiestreatment of pediatric acute lymphoblastic leukemiaautophagystress conditionsviability assayautophagy inhibitorleukemic cellscellstumor cells
2023
Deep thermal profiling for detection of functional proteoform groups
Kurzawa N, Leo I, Stahl M, Kunold E, Becher I, Audrey A, Mermelekas G, Huber W, Mateus A, Savitski M, Jafari R. Deep thermal profiling for detection of functional proteoform groups. Nature Chemical Biology 2023, 19: 962-971. PMID: 36941476, PMCID: PMC10374440, DOI: 10.1038/s41589-023-01284-8.Peer-Reviewed Original ResearchConceptsfunctional annotationthermal proteome profilingacute lymphoblastic leukemia cell linelymphoblastic leukemia cell lineleukemia cell linesfunctional proteomicscellular physiologypost-translationallyco-aggregationproteomic profilingfunctional rolecell linescytogenetic aberrationsproteoformsdisease biologydrug sensitivitygenomeproteomicsgenesprotein
2022
Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines
Leo I, Aswad L, Stahl M, Kunold E, Post F, Erkers T, Struyf N, Mermelekas G, Joshi R, Gracia-Villacampa E, Östling P, Kallioniemi O, Tamm K, Siavelis I, Lehtiö J, Vesterlund M, Jafari R. Integrative multi-omics and drug response profiling of childhood acute lymphoblastic leukemia cell lines. Nature Communications 2022, 13: 1691. PMID: 35354797, PMCID: PMC8967900, DOI: 10.1038/s41467-022-29224-5.Peer-Reviewed Original ResearchConceptsacute lymphoblastic leukemiadrug response profilesintegrative multi-omicscomprehensive multi-omics analysisacute lymphoblastic leukemia casescell lineshigh-risk subtypesacute lymphoblastic leukemia cell linemulti-omics analysislymphoblastic leukemia cell linedrug sensitivity profilesresponse to therapysubsets of patientsALL cell linesleukemia cell linesomics resourcestranscriptome profilingMulti-Omicslymphoblastic leukemiadrug responsepoor responsenegative selectionmolecular phenotypesoncology drugsLiver stromal cells restrict macrophage maturation and stromal IL-6 limits the differentiation of cirrhosis-linked macrophages
Buonomo E, Mei S, Guinn S, Leo I, Peluso M, Nolan M, Schildberg F, Zhao L, Lian C, Xu S, Misdraji J, Kharchenko P, Sharpe A. Liver stromal cells restrict macrophage maturation and stromal IL-6 limits the differentiation of cirrhosis-linked macrophages. Journal Of Hepatology 2022, 76: 1127-1137. PMID: 35074474, DOI: 10.1016/j.jhep.2021.12.036.Peer-Reviewed Original ResearchConceptsliver stromal cellssingle-cell RNA sequencing analysisstromal cellsliver diseaseIL-6IL-6 productioncoculture systemmyeloid cellsmacrophage maturationRNA sequencing analysisliver tissuemacrophage differentiationhuman liver tissueAbstractText Label="Background &context of cirrhosisIL-6 levelspathogenesis of liver diseaseflow cytometric analysishealthy liver tissue