2001
Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato.
Castro M, Nicholson W, Kramer V, Childs J. Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato. American Journal Of Tropical Medicine And Hygiene 2001, 65: 261-7. PMID: 11693866, DOI: 10.4269/ajtmh.2001.65.261.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialArachnid VectorsBase SequenceBorrelia burgdorferiCaliforniaDisease ReservoirsDNA, BacterialEhrlichiaEhrlichiosisFemaleFluorescent Antibody Technique, IndirectHeat-Shock ProteinsHumansIxodesMaleMicePolymerase Chain ReactionRatsRodent DiseasesSeasonsSeroepidemiologic StudiesSigmodontinaeZoonosesConceptsPolymerase chain reaction testingGroESL heat shock operonPersistent infectionReaction testingRatsE. phagocytophilaLyme diseaseBorrelia burgdorferiMiceSeropositive animalsDusky-footed wood ratsPCRWood ratsSeroreversionSensu latoSeroconversionSeropositiveBiopsySerologySeroreactivityInfectionDiseaseBloodAdult Ixodes pacificus CooleySerumInfections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus
Paddock C, Folk S, Shore G, Machado L, Huycke M, Slater L, Liddell A, Buller R, Storch G, Monson T, Rimland D, Sumner J, Singleton J, Bloch K, Tang Y, Standaert S, Childs J. Infections with Ehrlichia chaffeensis and Ehrlichia ewingii in Persons Coinfected with Human Immunodeficiency Virus. Clinical Infectious Diseases 2001, 33: 1586-1594. PMID: 11568857, DOI: 10.1086/323981.Peer-Reviewed Original ResearchMeSH KeywordsAdultEhrlichiaEhrlichia chaffeensisEhrlichiosisFemaleHIV InfectionsHIV-1HumansMaleMiddle AgedUnited StatesConceptsHuman immunodeficiency virusImmunodeficiency virusE. ewingiiE. chaffeensisCases of ehrlichiosisDiagnosis of ehrlichiosisT-lymphocyte countsCells/microL.Ehrlichia ewingiiEhrlichia chaffeensisLife-threatening illnessLymphocyte countClinical courseHIV infectionMedical managementPatient cohortSevere manifestationsPatientsLaboratory evaluationEhrlichiosisChaffeensisInfectionEwingiiMultiple factorsVirusClinical and Serological Follow-Up of Patients with Human Granulocytic Ehrlichiosis in Slovenia
Lotrič-Furlan S, Avsic-Zupanc T, Petrovec M, Nicholson W, Sumner J, Childs J, Strle F. Clinical and Serological Follow-Up of Patients with Human Granulocytic Ehrlichiosis in Slovenia. MSphere 2001, 8: 899-903. PMID: 11527800, PMCID: PMC96168, DOI: 10.1128/cdli.8.5.899-903.2001.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisAntibody titersTick biteLong-term clinical consequencesGranulocytic ehrlichiosisIFA antibody titersOnset of feverSerological Follow-UpHigh antibody titersMost time pointsSeronegative patientsSeropositive patientsAdult patientsFebrile illnessPatients 6Initial presentationClinical outcomesAntibody responseClinical evaluationFollow-upReciprocal titersClinical consequencesSerum collectionFirst presentationPatientsProspective Assessment of the Etiology of Acute Febrile Illness after a Tick Bite in Slovenia
Lotrič-Furlan S, Petrovec M, Avsic-Zupanc T, Nicholson W, Sumner J, Childs J, Strle F. Prospective Assessment of the Etiology of Acute Febrile Illness after a Tick Bite in Slovenia. Clinical Infectious Diseases 2001, 33: 503-510. PMID: 11462187, DOI: 10.1086/322586.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAnimalsBites and StingsBorrelia burgdorferi GroupCohort StudiesEhrlichiaEhrlichia chaffeensisEhrlichiosisEncephalitis Viruses, Tick-BorneEncephalitis, Tick-BorneFemaleFeverHumansLyme DiseaseMaleMiddle AgedProspective StudiesSloveniaTick-Borne DiseasesTicksConceptsFebrile illnessTick biteEhrlichia phagocytophila infectionAcute febrile illnessTickborne diseasesEvidence of infectionBorrelia burgdorferi sensu latoMeningeal involvementProspective studyClinical criteriaBurgdorferi sensu latoTickborne encephalitisProspective assessmentPatientsLaboratory evidenceImportant causeBacterial infectionsIllnessLyme borreliosisInfectionDiseaseResidents of SloveniaEtiologyBiteEncephalitisFort Chaffee Revisited: The Epidemiology of Tick-Borne Rickettsial and Ehrlichial Diseases at a Natural Focus
McCall C, Curns A, Rotz L, Singleton J, Treadwell T, Comer J, Nicholson W, Olson J, Childs J. Fort Chaffee Revisited: The Epidemiology of Tick-Borne Rickettsial and Ehrlichial Diseases at a Natural Focus. Vector-Borne And Zoonotic Diseases 2001, 1: 119-127. PMID: 12653142, DOI: 10.1089/153036601316977723.Peer-Reviewed Original ResearchConceptsTick-borne pathogensSerum samplesRetrospective cohort studyDetectable antibody titersPrimary risk factorRisk of transmissionFort ChaffeeFever group rickettsiaeCompatible illnessReactive EIAsTick-borne diseaseCohort studyDoxycycline useAntibody titersRisk factorsTick biteEhrlichial diseasesGroup rickettsiaeDiseaseMore pathogensProbable diseaseSeropositivityIllnessEhrlichia speciesInfection
2000
DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON
Comer J, Nicholson W, Paddock C, Sumner J, Childs J. DETECTION OF ANTIBODIES REACTIVE WITH EHRLICHIA CHAFFEENSIS IN THE RACCOON. Journal Of Wildlife Diseases 2000, 36: 705-712. PMID: 11085432, DOI: 10.7589/0090-3558-36.4.705.Peer-Reviewed Original ResearchSurvival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs
McKechnie D, Slater K, Childs J, Massung R, Paddock C. Survival of Ehrlichia chaffeensis in refrigerated, ADSOL‐treated RBCs. Transfusion 2000, 40: 1041-1047. PMID: 10988303, DOI: 10.1046/j.1537-2995.2000.40091041.x.Peer-Reviewed Original ResearchConceptsE. chaffeensisNucleated cellsTransfusion-acquired infectionEhrlichia chaffeensisMonocytic cell line THP-1Cell line THP-1Infected cell linesCytocentrifuged slidesTransfusion transmissionPacked RBC unitsPCR positivityE. chaffeensis DNASupernatant fractionCell culturesWeekly examinationsRBC unitsStudy designRBC productsTHP-1DH82 cellsPCR resultsCell linesChaffeensisInfectionSuccessful reisolationPrimary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics
Standaert S, Yu T, Scott M, Childs J, Paddock C, Nicholson W, Singleton J, Blaser M. Primary Isolation of Ehrlichia chaffeensis from Patients with Febrile Illnesses: Clinical and Molecular Characteristics. The Journal Of Infectious Diseases 2000, 181: 1082-1088. PMID: 10720534, DOI: 10.1086/315346.Peer-Reviewed Original ResearchConceptsPolymerase chain reactionE. chaffeensisCerebrospinal fluid specimensCultures of bloodHigh diagnostic yieldEhrlichia chaffeensisPrimary isolationFebrile illnessDiagnostic titerBlood isolatesDiagnostic yieldTick exposureSame patientCerebrospinal fluidPatientsFluid specimensDiagnostic testsFrequent isolationBloodChain reactionChaffeensisInfectionMolecular characteristicsMolecular analysisIsolates
1999
The Human Ehrlichioses in the United States - Volume 5, Number 5—October 1999 - Emerging Infectious Diseases journal - CDC
McQuiston J, Paddock C, Holman R, Childs J. The Human Ehrlichioses in the United States - Volume 5, Number 5—October 1999 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 1999, 5: 635-642. PMID: 10511519, PMCID: PMC2627718, DOI: 10.3201/eid0505.990504.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisHuman monocytic ehrlichiosisInfectious Diseases journal - CDCState health departmentsPublic health importanceSerologic testingNational surveillanceHealth departmentsHuman ehrlichiosesHealth importanceDisease controlGranulocytic ehrlichiosisMonocytic ehrlichiosisEhrlichiosisUpper midwestern statesAnnual numberBetter assessmentCasesPreventionOutcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance
Childs J, Sumner J, Nicholson W, Massung R, Standaert S, Paddock C. Outcome of Diagnostic Tests Using Samples from Patients with Culture-Proven Human Monocytic Ehrlichiosis: Implications for Surveillance. Journal Of Clinical Microbiology 1999, 37: 2997-3000. PMID: 10449489, PMCID: PMC85432, DOI: 10.1128/jcm.37.9.2997-3000.1999.Peer-Reviewed Original ResearchConceptsHuman monocytic ehrlichiosisDiagnosis of HMEConvalescent-phase serum samplesMonocytic ehrlichiosisDetection of morulaeInitial clinical presentationCulture-proven casesE. chaffeensis antigenWhole blood specimensCompatible illnessClinical presentationInitial presentationSerologic responseCase definitionHME patientsBlood specimensImmunoglobulin MPatientsBlood smearsIndirect immunofluorescenceEhrlichial DNADiagnostic testsSerum samplesProbable diseaseEhrlichia chaffeensisSerologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center
Comer J, Nicholson W, Olson J, Childs J. Serologic Testing for Human Granulocytic Ehrlichiosis at a National Referral Center. Journal Of Clinical Microbiology 1999, 37: 558-564. PMID: 9986812, PMCID: PMC84468, DOI: 10.1128/jcm.37.3.558-564.1999.Peer-Reviewed Original ResearchConceptsHGE agentSerum samplesNational referral centerE. chaffeensis antigenSpecific etiologic agentState health departmentsE. chaffeensisHuman granulocytic ehrlichiosis (HGE) agentHuman granulocytic ehrlichiosisHuman monocytic ehrlichiosisReferral centerSerologic testingEhrlichial antigenAverage ageHealth departmentsHuman ehrlichiosisPatientsEtiologic agentProbable casesHigh titersIll individualsGranulocytic ehrlichiosisDisease controlIndirect immunofluorescenceMonocytic ehrlichiosisDiagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum
Comer J, Nicholson W, Sumner J, Olson J, Childs J. Diagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum. Journal Of Clinical Microbiology 1999, 37: 31-34. PMID: 9854059, PMCID: PMC84159, DOI: 10.1128/jcm.37.1.31-34.1999.Peer-Reviewed Original ResearchConceptsDetermination of etiologyAcute-phase seraHuman granulocytic ehrlichiosisSerologic testingHuman ehrlichiosisAcute-phase serum samplesEarly detectionWhole blood specimensPCR assaysBlood specimensPCR testingEtiologic agentGranulocytic ehrlichiosisEtiologyHGE agentSimilar titersSerum samplesExposure historyTitersEhrlichia chaffeensisEhrlichiosisFourfold differenceE. chaffeensisSerumAntibodiesIdentity of Ehrlichial DNA Sequences Derived from Ixodes ricinus Ticks with Those Obtained from Patients with Human Granulocytic Ehrlichiosis in Slovenia
Petrovec M, Sumner J, Nicholson W, Childs J, Strle F, Barlič J, Lotrič-Furlan S, AvšičŽupanc T. Identity of Ehrlichial DNA Sequences Derived from Ixodes ricinus Ticks with Those Obtained from Patients with Human Granulocytic Ehrlichiosis in Slovenia. Journal Of Clinical Microbiology 1999, 37: 209-210. PMID: 9854093, PMCID: PMC84210, DOI: 10.1128/jcm.37.1.209-210.1999.Peer-Reviewed Original Research
1998
Human ehrlichiosis in central Europe.
Lotric-Furlan S, Petrovec M, Avsic-Zupanc T, Nicholson W, Sumner J, Childs J, Strle F. Human ehrlichiosis in central Europe. Wiener Klinische Wochenschrift 1998, 110: 894-7. PMID: 10048172.Peer-Reviewed Original ResearchMeSH KeywordsAcute DiseaseAdultAgedEhrlichia chaffeensisEhrlichiosisFemaleHumansMaleMiddle AgedSloveniaTick-Borne DiseasesConceptsFebrile illnessTick biteHGE agentPresence of infectionUniversity Medical CenterBlood smear examinationHuman granulocytic ehrlichiosis (HGE) agentEhrlichiosis casesAcute human diseaseClinical characteristicsAntibiotic therapyDisease coursePhysician awarenessProspective studyLaboratory findingsDifferential diagnosisMedical CenterSmear examinationHuman diseasesHuman ehrlichiosisNumber of casesPatientsHuman infectionsInfectious diseasesDiseaseHuman Granulocytic Ehrlichiosis in Europe: Clinical and Laboratory Findings for Four Patients from Slovenia
Lotrič-Furlan S, Petrovec M, Zupanc T, Nicholson W, Sumner J, Childs J, Strle F. Human Granulocytic Ehrlichiosis in Europe: Clinical and Laboratory Findings for Four Patients from Slovenia. Clinical Infectious Diseases 1998, 27: 424-428. PMID: 9770134, DOI: 10.1086/514683.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisHGE agentFebrile illnessLaboratory findingsTick biteAcute human granulocytic ehrlichiosisGranulocytic ehrlichiosisTick-borne diseaseAcute infectionAntibiotic therapyDisease coursePolymerase chain reaction analysisClinical featuresDetectable antibodiesDifferential diagnosisFirst visitChain reaction analysisPatientsHigh titersIllnessEhrlichial organismsEhrlichiosisAntibodiesBiteUnited StatesSerologic Evidence of Infection with Ehrlichia spp. in Wild Rodents (Muridae: Sigmodontinae) in the United States
Nicholson W, Muir S, Sumner J, Childs J. Serologic Evidence of Infection with Ehrlichia spp. in Wild Rodents (Muridae: Sigmodontinae) in the United States. Journal Of Clinical Microbiology 1998, 36: 695-700. PMID: 9508298, PMCID: PMC104611, DOI: 10.1128/jcm.36.3.695-700.1998.Peer-Reviewed Original ResearchRetrospective Serosurvey for Human Granulocytic Ehrlichiosis Agent in Urban White-footed Mice from Maryland
Bunnell J, Stephen D, Childs J, Glass G. Retrospective Serosurvey for Human Granulocytic Ehrlichiosis Agent in Urban White-footed Mice from Maryland. Journal Of Wildlife Diseases 1998, 34: 179-181. PMID: 9476244, DOI: 10.7589/0090-3558-34.1.179.Peer-Reviewed Original Research
1997
Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis
Paddock C, Sumner J, Shore G, Bartley D, Elie R, McQuade J, Martin C, Goldsmith C, Childs J. Isolation and characterization of Ehrlichia chaffeensis strains from patients with fatal ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 2496-2502. PMID: 9316896, PMCID: PMC229999, DOI: 10.1128/jcm.35.10.2496-2502.1997.Peer-Reviewed Original ResearchConceptsE. chaffeensisDH82 cellsEhrlichia chaffeensisArkansas isolateGroESL heat shock operonFatal ehrlichiosisMechanisms of diseasePulmonary insufficiencyClinical presentationMarked thrombocytopeniaSevere diseaseEhrlichial infectionCharacteristic manifestationsPatientsDays postinoculationMolecular evaluationChaffeensisPrimary isolationRRNA gene sequencesDiseaseIsolatesSpecific associationNew isolatesGene sequencesProtein geneAn indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis
Nicholson W, Comer J, Sumner J, Gingrich-Baker C, Coughlin R, Magnarelli L, Olson J, Childs J. An indirect immunofluorescence assay using a cell culture-derived antigen for detection of antibodies to the agent of human granulocytic ehrlichiosis. Journal Of Clinical Microbiology 1997, 35: 1510-1516. PMID: 9163471, PMCID: PMC229776, DOI: 10.1128/jcm.35.6.1510-1516.1997.Peer-Reviewed Original ResearchConceptsHuman granulocytic ehrlichiosisCell culture-derived antigenHuman antibodiesGranulocytic ehrlichiosisIndirect immunofluorescenceHGE agentAnimal-derived antigensDetection of antibodiesTick-derived isolatesPromyelocytic leukemia cell lineAntigen presentationHuman promyelocytic leukemia cell lineHealthy individualsLeukemia cell linesHGE patientsSera reactiveEhrlichia phagocytophilaHigh titersAntigenImmunofluorescence methodE. equiEhrlichia equiRoutine testingAntibodiesSpecific antibodies
1995
Ehrlichiosis in a Golf-Oriented Retirement Community
Standaert S, Dawson J, Schaffner W, Childs J, Biggie K, Singleton J, Gerhardt R, Knight M, Hutcheson R. Ehrlichiosis in a Golf-Oriented Retirement Community. New England Journal Of Medicine 1995, 333: 420-425. PMID: 7616991, DOI: 10.1056/nejm199508173330704.Peer-Reviewed Original ResearchConceptsE. chaffeensis infectionCase-control studySerologic testingCommunity AMedical history reviewLone star tickRisk of infectionCommunity BAnnual incidenceSerologic evidencePolymerase chain reactionTick biteRetirement communityInfectionEhrlichiosisEndemic diseaseChain reactionEhrlichia chaffeensisHigh rateSurvey participantsPercentTicksInsect repellentOutbreakRecreational activities