2001
Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato.
Castro M, Nicholson W, Kramer V, Childs J. Persistent infection in Neotoma fuscipes (Muridae: Sigmodontinae) with Ehrlichia phagocytophila sensu lato. American Journal Of Tropical Medicine And Hygiene 2001, 65: 261-7. PMID: 11693866, DOI: 10.4269/ajtmh.2001.65.261.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, BacterialArachnid VectorsBase SequenceBorrelia burgdorferiCaliforniaDisease ReservoirsDNA, BacterialEhrlichiaEhrlichiosisFemaleFluorescent Antibody Technique, IndirectHeat-Shock ProteinsHumansIxodesMaleMicePolymerase Chain ReactionRatsRodent DiseasesSeasonsSeroepidemiologic StudiesSigmodontinaeZoonosesConceptsPolymerase chain reaction testingGroESL heat shock operonPersistent infectionReaction testingRatsE. phagocytophilaLyme diseaseBorrelia burgdorferiMiceSeropositive animalsDusky-footed wood ratsPCRWood ratsSeroreversionSensu latoSeroconversionSeropositiveBiopsySerologySeroreactivityInfectionDiseaseBloodAdult Ixodes pacificus CooleySerum
1999
Dusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California
Nicholson W, Castro M, Kramer V, Sumner J, Childs J. Dusky-Footed Wood Rats (Neotoma fuscipes) as Reservoirs of Granulocytic Ehrlichiae (Rickettsiales: Ehrlichieae) in Northern California. Journal Of Clinical Microbiology 1999, 37: 3323-3327. PMID: 10488199, PMCID: PMC85556, DOI: 10.1128/jcm.37.10.3323-3327.1999.Peer-Reviewed Original ResearchConceptsBlood samplesBlood specimensDusky-footed wood ratsConcurrent high prevalenceSeronegative blood samplesHuman granulocytic ehrlichiosisGroESL heat shock operonGranulocytic ehrlichiaePCR-positive animalsPCR positivityHigh prevalencePCR-positive ticksSeropositive specimensRat blood samplesRatsGranulocytic ehrlichiosisEhrlichial DNAPacificus adultsEhrlichia equiEhrlichial agentsMicePCR assaysAdultsWood ratsRodentsMolecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation
Sumner J, Childs J, Paddock C. Molecular Cloning and Characterization of the Ehrlichia chaffeensis Variable-Length PCR Target: an Antigen-Expressing Gene That Exhibits Interstrain Variation. Journal Of Clinical Microbiology 1999, 37: 1447-1453. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensis
1995
Ehrlichiosis in a Golf-Oriented Retirement Community
Standaert S, Dawson J, Schaffner W, Childs J, Biggie K, Singleton J, Gerhardt R, Knight M, Hutcheson R. Ehrlichiosis in a Golf-Oriented Retirement Community. New England Journal Of Medicine 1995, 333: 420-425. PMID: 7616991, DOI: 10.1056/nejm199508173330704.Peer-Reviewed Original ResearchConceptsE. chaffeensis infectionCase-control studySerologic testingCommunity AMedical history reviewLone star tickRisk of infectionCommunity BAnnual incidenceSerologic evidencePolymerase chain reactionTick biteRetirement communityInfectionEhrlichiosisEndemic diseaseChain reactionEhrlichia chaffeensisHigh rateSurvey participantsPercentTicksInsect repellentOutbreakRecreational activities
1994
Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis
Dawson J, Stallknecht D, Howerth E, Warner C, Biggie K, Davidson W, Lockhart J, Nettles V, Olson J, Childs J. Susceptibility of white-tailed deer (Odocoileus virginianus) to infection with Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Journal Of Clinical Microbiology 1994, 32: 2725-2728. PMID: 7852563, PMCID: PMC264150, DOI: 10.1128/jcm.32.11.2725-2728.1994.Peer-Reviewed Original ResearchConceptsHuman ehrlichiosisE. canisE. chaffeensisEtiologic agentAbsence of seroconversionEhrlichia chaffeensisMacrophage-type cellsE. chaffeensis infectionLymph nodesPeripheral bloodClinical diseaseDay 10InfectionEhrlichia canisEhrlichiosisAntibodiesWhite-tailed deerChaffeensisNegative controlCanisReservoir hostsCompetent reservoirsWeeksTick vectorSeroconversionSerologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States
Childs J, Ksiazek T, Spiropoulou C, Krebs J, Morzunov S, Maupin G, Gage K, Rollin P, Sarisky J, Enscore R, Frey J, Peters C, Nichol S. Serologic and Genetic Identification of Peromyscus maniculatus as the Primary Rodent Reservoir for a New Hantavirus in the Southwestern United States. The Journal Of Infectious Diseases 1994, 169: 1271-1280. PMID: 8195603, DOI: 10.1093/infdis/169.6.1271.Peer-Reviewed Original Research
1993
Genetic Identification of a Hantavirus Associated with an Outbreak of Acute Respiratory Illness
Nichol S, Spiropoulou C, Morzunov S, Rollin P, Ksiazek T, Feldmann H, Sanchez A, Childs J, Zaki S, Peters C. Genetic Identification of a Hantavirus Associated with an Outbreak of Acute Respiratory Illness. Science 1993, 262: 914-917. PMID: 8235615, DOI: 10.1126/science.8235615.Peer-Reviewed Original Research
1992
Comparison of polymerase chain reaction and culture for detection of Borrelia burgdorferi in naturally infected Peromyscus leucopus and experimentally infected C.B-17 scid/scid mice
Hofmeister E, Markham R, Childs J, Arthur R. Comparison of polymerase chain reaction and culture for detection of Borrelia burgdorferi in naturally infected Peromyscus leucopus and experimentally infected C.B-17 scid/scid mice. Journal Of Clinical Microbiology 1992, 30: 2625-2631. PMID: 1400962, PMCID: PMC270489, DOI: 10.1128/jcm.30.10.2625-2631.1992.Peer-Reviewed Original Research