2008
A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses
Kamir D, Zierow S, Leng L, Cho Y, Diaz Y, Griffith J, McDonald C, Merk M, Mitchell RA, Trent J, Chen Y, Kwong YK, Xiong H, Vermeire J, Cappello M, McMahon-Pratt D, Walker J, Bernhagen J, Lolis E, Bucala R. A Leishmania Ortholog of Macrophage Migration Inhibitory Factor Modulates Host Macrophage Responses. The Journal Of Immunology 2008, 180: 8250-8261. PMID: 18523291, PMCID: PMC2668862, DOI: 10.4049/jimmunol.180.12.8250.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntigens, Differentiation, B-LymphocyteApoptosis Regulatory ProteinsCell LineCells, CulturedCrystallography, X-RayHistocompatibility Antigens Class IIHumansIntramolecular OxidoreductasesLeishmania majorMacrophage Migration-Inhibitory FactorsMacrophages, PeritonealMiceMice, Inbred BALB CMice, Inbred C3HMice, KnockoutMolecular Sequence DataRecombinant ProteinsStructural Homology, ProteinConceptsMacrophage migration inhibitory factorCD74-dependent mannerMigration inhibitory factorImmune defense mechanismsHuman macrophage migration inhibitory factorSmall molecule antagonistsActivation-induced apoptosisHost macrophage responseMIF receptorMIF proteinImmune destructionObligate intracellular parasitesMAP kinase activationERK1/2 MAP kinase activationInhibitory factorMacrophage responseLeishmania majorIntracellular parasitesHigh-resolution X-ray crystal structuresSpecies-specific inhibitionMacrophagesSignificant structural homologyKinase activationDefense mechanismsMammalian counterparts
1992
Cutaneous Leishmaniasis: Review of 59 Cases Seen at the National Institutes of Health
Melby P, Kreutzer R, McMahon-Pratt D, Gam A, Neva F. Cutaneous Leishmaniasis: Review of 59 Cases Seen at the National Institutes of Health. Clinical Infectious Diseases 1992, 15: 924-937. PMID: 1457663, DOI: 10.1093/clind/15.6.924.Peer-Reviewed Original ResearchConceptsCutaneous leishmaniasisEndemic areasCutaneous diseaseGroup of patientsDiffuse cutaneous leishmaniasisNational InstituteDifferent endemic areasChronic relapsingFrequent international travelOptimal therapyClinical spectrumMultiple lesionsAmerican patientsLeishmaniasisPatientsLeishmania majorDiseaseLesionsEarly ageInternational travelU.S. residentsHealthRelapsingMajorityTherapyLoss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex
McMahon-Pratt D, Traub-Cseko Y, Lohman K, Rogers D, Beverley S. Loss of the GP46/M-2 surface membrane glycoprotein gene family in the Leishmania braziliensis complex. Molecular And Biochemical Parasitology 1992, 50: 151-160. PMID: 1542309, DOI: 10.1016/0166-6851(92)90252-f.Peer-Reviewed Original ResearchConceptsGP46/MGene familyGlycoprotein gene familyMolecular karyotype analysisBraziliensis complexLeishmania braziliensis complexSpeciation eventsTrypanosomatid speciesPhylogenetic analysisCrithidia fasciculataLeishmania tarentolaeDevelopmental cycleWestern blot analysisLeishmania mexicana complexMembrane glycoproteinsKaryotype analysisBlot analysisL. braziliensis complexProtein expressionPolyclonal antiserumMexicana complexSpeciesL. panamensisLeishmania majorL. braziliensis
1990
Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.
LeBowitz J, Coburn C, McMahon-Pratt D, Beverley S. Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 9736-9740. PMID: 2124701, PMCID: PMC55248, DOI: 10.1073/pnas.87.24.9736.Peer-Reviewed Original ResearchConceptsExpression vectorCis-acting elementsTrypanosomatid protozoan parasitesRNA editingSurface antigen geneMolecular paradigmProcessing eventsParasite proteinsGenetic analysisLeishmania expression vectorExpression sitesPathogenic speciesNormal transcriptMembrane glycoproteinsEscherichia coliGenesImmunoblot analysisProtozoan parasiteProteinAntigen geneColony assayLeishmania majorFamily membersL. amazonensisKilobases