2022
Characterization of hearing-impairment in Generalized Arterial Calcification of Infancy (GACI)
Theng EH, Brewer CC, Oheim R, Zalewski CK, King KA, Delsmann MM, Rolvien T, Gafni RI, Braddock DT, Jeffrey Kim H, Ferreira CR. Characterization of hearing-impairment in Generalized Arterial Calcification of Infancy (GACI). Orphanet Journal Of Rare Diseases 2022, 17: 273. PMID: 35854274, PMCID: PMC9295326, DOI: 10.1186/s13023-022-02410-w.Peer-Reviewed Original ResearchConceptsOssicular chain dysfunctionGeneralized Arterial CalcificationOtitis mediaArterial calcificationPrevalence of HLRecurrent otitis mediaTerms of patientTemporal bone imagingEtiology of HLBasic science laboratoryAuricular calcificationConductive HLHL etiologyOtologic featuresDegree of HLCohort studyOtologic evaluationRadiologic featuresAudiological assessmentAuditory dysfunctionTube placementRecurrent episodesBilateral HLCranial CTLikely multifactorial
2019
Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold
Braddock D, Stabach P, Zimmerman K, Kavanagh D, Sauei C, Yarema K. Protein Engineering and Glycan Optimization Improves Pharmicokinetics of an Enzyme Biologic 10‐fold. The FASEB Journal 2019, 33: 801.1-801.1. DOI: 10.1096/fasebj.2019.33.1_supplement.801.1.Peer-Reviewed Original ResearchFusion proteinProtein engineeringCHO cell linesCellular recyclingBioprocessing methodsRecombinant fusion proteinConsensus sequenceExtracellular domainSialic acidTertiary structureN-acetylmannosamineCHO cellsHuman alphaProteinCell linesFASEB JournalFc domainCell culture mediumCulture mediumGeneralized Arterial CalcificationENPP1
2018
ENPP1 Enzyme Replacement Prevents the Osteomalacia and Paradoxical Mineralization in the Enpp1asj/asj mouse model of Autosomal Recessive Hypophosphatemic Rickets Type‐2.
Braddock D, Kavanagh D, Li X, Carpenter T, Levine M, Horowitz M. ENPP1 Enzyme Replacement Prevents the Osteomalacia and Paradoxical Mineralization in the Enpp1asj/asj mouse model of Autosomal Recessive Hypophosphatemic Rickets Type‐2. The FASEB Journal 2018, 32: 816.13-816.13. DOI: 10.1096/fasebj.2018.32.1_supplement.816.13.Peer-Reviewed Original ResearchAutosomal recessive hypophosphatemic rickets type 2High-phosphate dietThinner growth platesBone mineral densityTrabecular bone volumePhosphate dietGrowth plate thicknessHistologic changesMineral densityOld miceProximal tibiaTissue calcificationBone volumeType 2Growth plateSoft tissue calcificationExperimental cohortEnzyme replacement therapyGeneralized Arterial CalcificationTrabecular boneFull-text articlesOsteopenic bone phenotypePost-yield deflectionHistologic hallmarkVascular calcification