2017
Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes
Dankwa S, Chaand M, Kanjee U, Jiang RHY, Nobre LV, Goldberg JM, Bei AK, Moechtar MA, Grüring C, Ahouidi AD, Ndiaye D, Dieye TN, Mboup S, Weekes MP, Duraisingh MT. Genetic Evidence for Erythrocyte Receptor Glycophorin B Expression Levels Defining a Dominant Plasmodium falciparum Invasion Pathway into Human Erythrocytes. Infection And Immunity 2017, 85: 10.1128/iai.00074-17. PMID: 28760933, PMCID: PMC5607420, DOI: 10.1128/iai.00074-17.Peer-Reviewed Original ResearchConceptsInvasion pathwaysGlycophorin CGlycophorin BExpression levelsShort hairpin RNA transductionLigand-receptor interactionsErythroid progenitor cellsKnockout linesGenetic evidenceBioinformatics analysisKnockdown cellsProteomic profilingTranscript levelsMultiple proteinsHuman erythrocytesExtensive variationParasite invasionProgenitor cellsInvasion ligandsB expression levelsLaboratory strainsGPC receptorsCulture-adapted strainsPathwayReceptor usage
2013
Inhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway
Badiane AS, Bei AK, Ahouidi AD, Patel SD, Salinas N, Ndiaye D, Sarr O, Ndir O, Tolia NH, Mboup S, Duraisingh MT. Inhibitory Humoral Responses to the Plasmodium falciparum Vaccine Candidate EBA-175 Are Independent of the Erythrocyte Invasion Pathway. MSphere 2013, 20: 1238-1245. PMID: 23761656, PMCID: PMC3754519, DOI: 10.1128/cvi.00135-13.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayEBA-175Human antibodiesAffinity-purified human antibodiesBlood-stage vaccine candidateParasite strainsSialic acid-dependent mannerParasite invasionPresence of antibodiesErythrocyte-binding domainMultiple ligand-receptor interactionsEx vivo assaysPooled patient plasmaSenegalese parasitesAlternative invasion pathwaysHumoral responseVaccine candidatesPatient plasmaSenegalese patientsLigand-receptor interactionsClinical isolatesInvasion inhibitionVaccine designEx vivoFunctional inhibition
2007
Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal
Jennings CV, Ahouidi AD, Zilversmit M, Bei AK, Rayner J, Sarr O, Ndir O, Wirth DF, Mboup S, Duraisingh MT. Molecular Analysis of Erythrocyte Invasion in Plasmodium falciparum Isolates from Senegal. Infection And Immunity 2007, 75: 3531-3538. PMID: 17470537, PMCID: PMC1932937, DOI: 10.1128/iai.00122-07.Peer-Reviewed Original ResearchConceptsInvasion pathwaysSequence polymorphismsMolecular analysisDistinct invasion pathwaysC-terminal regionMalaria parasitesSpecific sequence variantsGene amplificationHuman malaria parasiteLigand-receptor interactionsExpression polymorphismsRepeat regionProtein expression levelsSequence deletionsHomolog familyMultiple ligand-receptor interactionsLarge sequence deletionsSequence variantsSignificant sequence polymorphismErythrocyte invasionErythrocyte receptorP. falciparum parasitesExpression levelsPfRh1Low transmission areas