2017
Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy
Wisnewski AV, Kanyo J, Asher J, Goodrich JA, Barnett G, Patrylak L, Liu J, Redlich CA, Nassar AF. Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy. Xenobiotica 2017, 48: 488-497. PMID: 28489470, PMCID: PMC5863241, DOI: 10.1080/00498254.2017.1329569.Peer-Reviewed Original ResearchConceptsCollision-induced dissociation (CID) fragmentation patternsReaction productsLower airwaysChemical reactivityLow molecular weight fractionWeight fractionHigh molecular weight fractionAliphatic diisocyanateMolecular weight fractionFragmentation patternsHexamethylenediisocyanateLC-MSHDI vaporAirways of rabbitsBronchoalveolar lavage fluidRabbit bronchoalveolar lavage fluidMoleculesDiisocyanate vaporsMs. 4Albumin. 5Occupational asthmaReactivityBAL fluidAsthma pathogenesisLavage fluid
2015
Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ
Wisnewski AV, Liu J. Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ. Journal Of Immunological Methods 2015, 429: 60-65. PMID: 26690039, PMCID: PMC4753098, DOI: 10.1016/j.jim.2015.12.008.Peer-Reviewed Original ResearchConceptsKeyhole limpet hemocyaninFreund's adjuvantDiisocyanate-induced asthmaComplete Freund's adjuvantIncomplete Freund's adjuvantLung tissue sectionsFormalin-fixed paraffinOccupational asthmaLower airwaysBooster injectionOccupational allergensAirway cellsAlveolar macrophagesLimpet hemocyaninAdjuvantHealth effectsTissue distributionTissue samplesTissue sectionsAsthmaDot blot analysisFurther studiesImmunochemical studiesBlot analysisEpithelial regions
2001
Development of immunoassays for biomonitoring of hexamethylene diisocyanate exposure.
Lemus R, Lukinskeine L, Bier ME, Wisnewski AV, Redlich CA, Karol MH. Development of immunoassays for biomonitoring of hexamethylene diisocyanate exposure. Environmental Health Perspectives 2001, 109: 1103-1108. PMID: 11712993, PMCID: PMC1240469, DOI: 10.1289/ehp.011091103.Peer-Reviewed Original ResearchConceptsHexamethylene diisocyanateHuman serum albuminLaser desorption timeMatrix-assisted laser desorption timeFlight mass spectrometryWorkplace air monitoringMass spectrometryDesorption timeDiisocyanateAdductsDevelopment of immunoassaysHSA antigenSerum albuminMicrotiter platesReactionAir monitoringHexamethylene diisocyanate exposurePpbAbsence of reactionSensitive bioassayNumerous samplesSpectrometryDiphenylPolyurethaneCoatings