2022
Glutathione reactivity with aliphatic polyisocyanates
Wisnewski AV, Liu J. Glutathione reactivity with aliphatic polyisocyanates. PLOS ONE 2022, 17: e0271471. PMID: 35839242, PMCID: PMC9286259, DOI: 10.1371/journal.pone.0271471.Peer-Reviewed Original ResearchConceptsHexamethylene diisocyanateAliphatic polyisocyanatesReaction productsMS/MSTandem MS/MSReversed-phase LC-MSPositive ion modeMajor reaction productsLiquid chromatography-coupled mass spectrometryCoupled-mass spectrometryPolymeric formulationsIon modeGlutathione reactivityFragmentation patternsGSH reactivityS linkageO groupLC-MSPolyisocyanatesIonsIsocyanate productsDiisocyanateImportant productsReactivityMS
2017
Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy
Wisnewski AV, Kanyo J, Asher J, Goodrich JA, Barnett G, Patrylak L, Liu J, Redlich CA, Nassar AF. Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy. Xenobiotica 2017, 48: 488-497. PMID: 28489470, PMCID: PMC5863241, DOI: 10.1080/00498254.2017.1329569.Peer-Reviewed Original ResearchConceptsCollision-induced dissociation (CID) fragmentation patternsReaction productsLower airwaysChemical reactivityLow molecular weight fractionWeight fractionHigh molecular weight fractionAliphatic diisocyanateMolecular weight fractionFragmentation patternsHexamethylenediisocyanateLC-MSHDI vaporAirways of rabbitsBronchoalveolar lavage fluidRabbit bronchoalveolar lavage fluidMoleculesDiisocyanate vaporsMs. 4Albumin. 5Occupational asthmaReactivityBAL fluidAsthma pathogenesisLavage fluid
2012
Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin
Wisnewski AV, Mhike M, Hettick JM, Liu J, Siegel PD. Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin. Toxicology In Vitro 2012, 27: 662-671. PMID: 23178851, PMCID: PMC3587365, DOI: 10.1016/j.tiv.2012.11.013.Peer-Reviewed Original ResearchConceptsHDI vaporCommon occupational allergensRespiratory tract exposureHuman albuminLevels of GSHReaction productsGSH concentrationVapor exposureIntra-molecular cyclizationClinical responseOccupational allergensAsthma pathogenesisPolyclonal rabbit serumAirway fluidSerologic assaysLower GSH concentrationsVivo reactivityRabbit serumPotential mechanismsGlutathione reactivityMass spectrometryExposureReactivityGSH solutionKey step